the 1

No immunostaining was seen in RPE. Double staining that Kir2. 1 and glutamine synthetase, Kir2. 1 and glutamine synthetase colocalized well. Intense Kir7. 1 immunolabeling was present on the apical surface of all RPE cells and appeared to extend over the length of the apical processes. Na〓, K〓-ATPase expression varied among RPE cells, but in highly expressing cells, it co-localized with Kir7. 1. Immunoreactivity of Kir2. 1、Kir4. 1 and Kir7. 1 acomplished by ABC immunohistochemistry in monkey and human retinae got the nearly same results as bovine.

间接免疫荧光组织化学显示，Kir2.1蛋白主要分布在Müller细胞与神经元相接触的细胞膜区域；与Müller细胞的特异性标记蛋白质抗谷氨酸合成酶抗体双重标记显示其分布特性重叠，在RPE未检测Kir2.1蛋白的免疫活性存在；Kir4.1蛋白集中分布于Müller细胞的内侧突起，与GS蛋白双重标记显示其分布特性重叠，RPE未检测Kir4.1蛋白的免疫活性存在；Kir7.1主要分布于RPE游离面及其突起的全长，与特异性标记RPE突起的Ezrin蛋白完全重叠，Na〓-K〓ATP酶在不同部位的RPE表达不同，Na〓-K〓ATP高表达的RPE细胞与Kir7.1的分布特性重叠。

Experiments show that:when the concentration of acetone is decreased from 100% to 20%,the absorbance bands at 3538cm-1 and 3441cm-1 of CH3 is redshifted,the absorbance band at 1713cm-1 and 1647cm-1 of CO is redshifted about 19 cm-1 and 21cm-1 respectively.

发现随着丙酮水溶液浓度的减小，丙酮CH3键的对称(3538cm-1)和不对称伸缩振动(3441cm-1)吸收峰位置波数减小方向迁徙，CO伸缩振动吸收(1713cm-1)向波数减小方向移动，丙酮浓度为20％时，迁徒至1691cm-1，减小了约19cm-1；同时1647cm-1处的吸收则迁徙至1626cm-1。

Demolition 1.1 The demolition of lifting mechanism 1.1.1 demolition handle pin, handle, fork rod pin, fork rod, valve pin, valve stem and stem nut locking pin; 1.2 regulate the demolition of ring 1.2.1 unloaded ring and nozzle ring adjustment screw, recording the number of alveolar nozzle ring nozzle ring used to turn to the right (anti-clockwise direction) until the stent in contact with the valve flap, recording the number of rotating shafts of the methods.

拆卸 1.1 提升机构的拆卸 1.1.1 拆手柄销、手柄、叉杆销、叉杆、阀销、阀杆锁紧销及阀杆螺母； 1.2 调节环的拆卸 1.2.1 卸下调节环和喷咀环的固定螺丝，记录喷咀环的齿槽数采用将喷咀环向右转（反时针方向直至与阀瓣支架相接触，记录所旋转的槽数的方法来实现。

During the course of the initial 1.5ML layer-by-layer growth, the first 0.5ML silver atoms fill in the missingrow sites of the Pt (110)-(1×2) surface and form a pseudo Pt (110)-(1×1)-0.5ML Pt0.5ML Ag surface structure, then the subsequent 0.5ML and the last 0.5ML silver atoms locate on the silver sites and the platinum sites of the pseudo (1×1) surface, respectively. At 600K, the formation of AgPt surface alloys follows the layer-bylayer growth of Ag thin film up to 2.25ML.

Ag原子在表面的定位非常有序，当0≤θ〓≤0.5ML，Ag原子填充Pt(110)-(1×2)表面的&missing-row&位并形成Pt(110)-(1×1)-0.5ML Pt-0.5ML Ag表面结构；当0.5ML≤θ〓≤1.0ML，Ag原子生长在Pt(110)-(1×1)表面的Ag位；当1.0ML≤θ〓≤1.5ML，Ag原子生长在Pt(110)-(1×1)表面的Pt位。600KAg薄膜在Pt(110)-(1×2)表面层层生长至2.25ML，然后形成表面合金。

Results Of the ADRs in 75 cases, the allergicreaction was the commonest one, 33 (44%), 19 reactions of nauseas and vomiting (25.3%), 13 phlebitis (17.3%), 4 dyspneas (5.3%), 3 diarrheas (4%), 1 dysuria(1.3%),1 dropsy (1.3%), 1 hematuria (1.3%).

结果75例老年人氟喹诺酮类药物所致不良反应中，以过敏反应最为常见，占33例(44%)，恶心呕吐19例(25.3%)，静脉炎13例(17.3%)，呼吸困难4例(5.3%)，腹泻3例(4%)，排尿困难1例(1.3%)，水肿1例(1.3%)，血尿1例(1.3%)。

The main physiological changes of yard-long bean stored at different temperature and the effect of plastic films, 6-BA and 1-MCP treatments on postharvest senescence physiology and edible quality changes were investigated. The results were as follows:1. Rate of chilling syndrome of yard-long bean appearing after 3d storage at 3 + 2 °C was 3% after 5d storage.

以'之豇-28'豇豆为试材，研究了贮藏温度3±2℃、8±2℃、12±2℃和常温(ck，26～37℃、薄膜包装(厚度为0.03mm的LDPE薄膜、0.02mm的专用保鲜膜、0.02mm的专用保鲜膜+直径10mm孔10个、ck)、6-BA处理10mg·Kg~(-1、20mg·Kg~(-1)、30mg·Kg~(-1)、0mg·Kg~(-1)、1-MCP处理0.5μL·L~(-1、1μL·L~(-1)、2μL·L~(-1)、0μL·L~(-1)对豇豆采后生理变化、贮藏品质及保鲜效果的影响，为豇豆贮藏保鲜技术的研究与应用提供理论依据和技术指导。

The method comprises: adding 4-(3,4-dichlorophenyl)-1-tetralone and solvent at a ratio of 1:(3-4) into a three-necked flask with a stirrer, heating under stirring until 4-(3,4-dichlorophenyl)-1-tetralone is completely dissolved, heating to 60-100 deg.C, refluxing for 0.5-2 h, cooling the filtrate to -5-10 deg.C, filtering, recrystallizing to obtain refined 4-(3,4-dichlorophenyl)-1-tetralone, and drying at 40-80 deg.C to obtain dry 4-(3,4-dichlorophenyl)-1-tetralone.

该方法的步骤是：在带搅拌的三颈瓶中依次加入4-(3，4-二氯苯基)-1-萘满酮和溶剂，溶剂与4-(3，4-二氯苯基)-1-萘满酮的L∶W为3～4∶1，将上述4-(3，4-二氯苯基)-1-萘满酮及混合精制溶剂搅拌下升温，使4-(3，4-二氯苯基)-1-萘满酮全部溶解，升温至60～100℃，回流0.5-2h；滤液用冰水冷却至-5～10℃，过滤，所得物料再用上述方法进行一次重结晶得到精品4-(3，4-二氯苯基)-1-萘满酮，40～80℃干燥后即得到干品4-(3，4-二氯苯基)-1-萘满酮。

It was established that, the fish skin was stirred two times with 2.5% or 5% NaCl solution at a ratio of material to solvent of 1:10 at about 5℃ for 12h, about 5.9%, 6.7%, 9.1% soluble non-collagenous protein could been separated from skin of silver carp, bighead carp and grass carp, then by homogenizing with acid at a ratio of material to solvent of 1:30, about 78.9%, 84.1%, 82.0% of collagen from skin of silver carp, grass carp and bighead carp could be extracted by acetic acid or citric acid.

结果表明，鲢、鱅鱼皮经2.5％NaCl溶液(1：10)，草鱼鱼皮经5％NaCl溶液(1：10)，温度5℃左右，12h磁力搅拌处理二次，除去的可溶性蛋白分别占原总蛋白的5.9％、6.7％、9.1％。鱼皮再经加酸(1：30)溶胀、均质处理后，即可得到鱼皮胶原蛋白的粗提取液，鲢、草鱼鱼皮的酸提取回收率以醋酸为最高，分别为78.9％、84.1％，鱅鱼皮以柠檬酸为最高，约为82.0％。

The experimental results show that: DNA of normal gastric mucosa has stable phosphate backbone; Since the peak intensity at 1090 cm^(-1) is lower than that at 1050 cm^(-1), phosphate backbone in DNA of intestinal metaplasia becomes unstable, while the rest spectra peaks are similar to those in normal gastric mucosa; In terms of adenocarcinoma DNA, double peaks at 1090 cm^(-1) whose intensity are higher than 1050 cm^(-1), are observed, which indicates that DNA chain is broken and re-forms a double stabilized phosphate backbones. In addition, peaks at 950 cm^(-1), 1010 cm^(-1), 1100~1600 cm^(-1) also exhibit obvious difference compared with normal gastric mucosa, which shows that deoxyribose and bases are changed due to broken of DNA.

实验结果表明，胃粘膜正常组织DNA中有稳定的磷酸骨架；肠化组织基因组DNA拉曼特征峰在1090 cm^(-1)处谱峰强度低于1050 cm^(-1)处谱峰强度，表明其磷酸骨架变得不稳定，其它位置的谱峰与正常组织相似；腺癌组织基因组DNA拉曼特征峰在1090 cm^(-1)附近出现双峰，其相对于1050 cm^(-1)处谱峰强度更强，提示DNA有可能出现断链并再次形成了稳定的磷酸骨架，在950 cm^(-1)、1010 cm^(-1)、1100~1600 cm^(-1)波段的特征谱峰与正常组织DNA相比变化也较大，说明脱氧核糖和碱基可能由于DNA断链也随之改变。

The control group were injected peritoneal physiological saline injection.Three study groups were injected acrylamide solution 5 mg.kg-1.d-1,30 mg.kg-1.d-1,and 60 mg.kg-1.d-1,respectively.On the 5th day,brains of each group were taken out quickly,fixed by Zamboni fixation solution,made into conventional freezing moviemaking,stained by immunohistochemistry staining,and then observed by light microscope.

对照组小鼠腹腔注射生理盐水；实验组小鼠分别代以5 mg·kg-1·d-1、30 mg·kg-1·d-1、60 mg·kg-1·d-1丙烯酰胺水溶液腹腔注射。5 d时分别处死各组小鼠，取出大脑，Zamboni固定液固定，常规冰冻制片，免疫组织化学染色，光镜观察。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。