查询词典 tetrazolium

Methods To irradiate the human epiderm keratinocytes with an identical dose of UVA,which is primary cultured and is occupying exponential growth phase.The matter interface is cystosepiment,tin foil and ice.The non- irradiation group is the control group.To detect the cells degree of injury through the improved methyl thiazolyl tetrazolium assay.

选取原代培养的处于指数生长期的角质形成细胞，分别采用泡沫板、锡纸板、冰面作为载物界面，进行相同剂量的UVA照射，同时设未照射组为对照组，通过改良MTT法检测细胞损伤程度。

The results indicated that the water or 50% ethanol extracts from peonies petals could reduce light absorption of DPPH and OH initiated hydroxylation of salicylate, and inhibit O2(superscript -) mediated light chemical reduction of nitroblue tetrazolium effectively, and lipid peroxidation of polyunsaturated fatty from yolk lipoprotein, in which DPPH scavenging activities of 6 peonies water extracts were hinger than that of 200 μg/mL BHT, but lower than 80 μg/mL Vc. In 4 kinds of detecting systems of antioxidation, the peroxidation activity of deep colored flowers of peonies was higher than that of light colored flowers of peonies.

牡丹花瓣提取液能明显抑制卵黄组织匀浆的脂质过氧化作用，降低DPPH的吸光值、抑制O2介导的NBT光化学还原及OH作用下的水杨酸羟基化作用，其中牡丹花瓣提取液清除DPPH自由基的活性高于200μg/mL BHT，但低于80μg/mL Vc.6个品种的牡丹花瓣提取液，在4种测定系统中均具有一定程度的抗氧化能力，且水提取液的抗氧化活性略高于体积分数50%乙醇提取液，深色花瓣提取液对不同自由基的清除能力较强。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant, which were called the wild tue strain group and the mutant stain group,respectively. The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.The induction of apoptosis was determined by flow cytometry.

将幽门螺杆菌野生株与cagA基因缺失突变株与BGC823细胞共培养，分别在6，12，24和48h观察细胞形态学变化，采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性，流式细胞仪检测细胞凋亡情况。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant,which were called the wild tue strain group and the mutant stain group,respectively.The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.The induction of apoptosis was determined by flow cytometry.

将幽门螺杆菌野生株和cagA基因缺失突变株和BGC823细胞共培养，分别在6，12，24和48h观察细胞形态学变化，采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性，流式细胞仪检测细胞凋亡情况。

The present study demonstrates that caffeic acid phenethyl ester, a major component of honeybee propolis, enhanced ATRA-induced granulocytic differentiation in HL-60, a human promyelocytic cell line. The differentiation was assessed by Wright-Giemsa stain, nitroblue tetrazolium reduction, and membrane differentiation marker CD11b.

经由实验结果发现，利用化学染剂(Wright-Giemsa stain)观察型态， CAPE也会促进低剂量 ATRA (1nM)分化的效用，而此种分化经由 NBT reduction，和透过流式细胞仪分析分化标的膜蛋白 CD11b/CD14及细胞周期测试后，证实细胞周期停滞 G1 phase与分化现象的机制有关；再藉由免疫沉降法测试，更证实细胞周期停滞将影响 CDK2的活性。

Methods: hRPE were cultured in normal (95% oxygeon) and hypoxia (2%-5% oxygeon) condition with DEX of different concentrations. The influence of DEX on cells growth and VEGF expression was detected by 3-(4,5-dimethylthiazole-2yl)-2,5diphenyl tetrazolium bromid and reverse transcriptionassay.

体外正常（氧浓度95%）和缺氧（氧浓度2%~5%）下培养视网膜色素上皮细胞，分别加入不同浓度地塞米松，用四唑溴盐和RT-PCR的方法，分别检测药物对细胞生长的影响和细胞VEGF的表达。

Methods The ARPE-19 cells were put into normal and hypoxic chamber respectively.After 0hr、8hr、24hr、48hr,the proliferation of ARPE-19 cells was evaluated by[3-(4,5-dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromid, MTT]-test.

第一部分缺氧人视网膜色素上皮细胞株ARPE-19表达特性目的观察缺氧对人视网膜色素上皮株ARPE-19的细胞形态、增殖以及对其分泌的血管内皮生长因子和色素上皮衍生因子表达的影响。

Methods The ARPE-19 cells were transfected with Mir-155,and then put into hypoxic chamber for 0hr、8hr、24hr、48hr,the proliferation of ARPE-19 cells was evaluated by[3-(4,5-dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromid, MTT]-test.

Mir-155转染视网膜色素上皮细胞株ARPE-19细胞后，将其置缺氧环境0hr、8hr、24hr、48hr后，MTT比色法检测ARPE-19细胞的增殖，流式细胞仪检测细胞凋亡率。

Five hours after the noise exposure, the cochleae were harvested. mitochondria energetic function, succinate dehydrogenase activity, in the hair cells of cochleae was evaluated with a colorimetric assay using blue tetrazolium monosodium salt. the sdh-labeled organs of corti were double stained with propidium iodide, a dna intercalating fluorescent probe used to visualize the morphologic viability of hair cell nuclei.

采用琥珀酸脱氢酶（succinate dehydrogenase，sdh）染色法进行耳蜗基底膜细胞线粒体染色，细胞核dna荧光染料碘化丙啶（propidium iodide，pi）双重染色耳蜗基底膜，以未受噪声暴露动物为对照，显微镜下观察噪声暴露后耳蜗基底膜核固缩和核肿胀毛细胞琥珀酸脱氢酶染色的变化。

Yunnanensis. The pollens of dehisced anthors of flowering P. polyphylla plants was slightly painted on the slide glass, the activities of P. polyphylla pollens were determined resp. by iodine-potassium iodide dyeing method (I2-KI), triphenyl tetrazolium chloride staining method, aceto carmine dyeing method and fluorescein diacetate method with a little improvement.

方法］将滇重楼开花植株裂开花药的花粉轻轻涂抹载玻片上，分别用碘－碘化钾(I2-KI)染色法、氯化三苯四氮唑染色法、醋酸洋红染色法和略有改动的荧光素二醋酸酯法对滇重楼花粉活力进行侧定。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。