查询词典 tetrazolium

Whats more, the model was appraised by behavioral test and Triphenyl Tetrazolium Choloride Staining.

最后利用行为学测试、四氮唑染色对模型成功与否进行判定。

The area at risk and infarct area were determined using Trypan blue and triphenyl tetrazolium choride staining respectively.

再灌末取心肌组织，以四氮唑染色法测定心肌梗死面积(IS/AAR×100%)。

The infarct size was measured by the triphenyl tetrazolium chloride method.

心肌梗塞面积通过三苯基四唑氯化物染色法测定。

In this paper, three methods were used to detemined the pollen viability of maize , including iodine-iodide kalium method, triphenyl tetrazolium chloridemethod and germination in vitro.

本文采用碘-碘化钾法，氯化三苯基四氮唑法及离体萌发法对花粉活力进行了室内测定，并与大田测定结果做了相关分析。

Methods The antimicrobial peptides of housefly larvae were induced largely by infection and injury, which were isolated and purified by trituration, centrifugalization and column chromatography. Then the antimicrobial peptides with anti-Toxoplasma activity were sieved by methyl thiazolyl tetrazolium colorimetric method and haemacytometry. The DNA contents of T.gondii tachyzoites were detected by flow cytometry.

通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，分离纯化并筛选出抗弓形虫作用的抗菌肽，采用流式细胞术分析其对弓形虫速殖子DNA含量的影响。

Four testing methods to detect the pollen viability of cucumber were being compared. The results indicated that I-KI staining method and acetic red dyeing were not suitable to judge the pollen viability of cucumber. Tetrazolium method can be used but the error is oversize. By and large, the Germination in vitro method was an efficient, stable and simple way to judge the pollen viability of cucumber. The component of culture medium is 10% sucrose+ 100mg/L H_3BO_3. If inoculated intraday, collect pollen of fresh abloom flower at 6:00 and if inoculated at the second day, collect before dark.

比较了4种常用的花粉活力测定方法——I-KI染色法、醋酸洋红染色法、TTC染色法和离体萌发法，认为I-KI染色法和醋酸洋红染色法不适于测定黄瓜花粉生活力，TTC染色法虽适用但误差较大，综合考虑各项因素，确定离体萌发法是最准确、简便、稳定的测定黄瓜花粉活力的方法。

Methods: Collected SKOV3 cells respectively in 24h、48h、72h under different concentrations of AS2O3. The inhibitive rate of cells was checked by Methyl thiazolyl tetrazolium assay to evaluate the effect of AS2O3. Telomerase activity of ovarian carcinoma cells was determined by the method of TRAP-PAGE argentation.

用不同浓度的AS2O3溶液作用卵巢癌细胞株SKOV3，于不同时间点收集细胞，MTT法检测细胞生长抑制率；TRAP-银染法观察端粒酶活性的变化。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Methods:The proliferation inhibition of cantharides injections on LoVo cells were detected by tetrazolium.Annexin V-FITC were applied to study cell apoptosis of LoVo cells.FCM were applied to detect the expressions of the Fas and Bcl-2 protein.Results:Cantharides iniections could inhibit the proliferation of LoVo cells and could couse cell apoptosis.

四甲基偶氮唑盐测定药物对LoVo细胞抑制作用与时间和剂量的关系，Annexin V-FITC方法对细胞凋亡进行检测，流式细胞术检测细胞Fas、Bcl-2蛋白表达水平结果：MTT实验表明复方斑蝥注射液可抑制LoVo细胞的生长并诱导发生凋亡。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。