查询词典 tetrameric

In attempt to obtain a further insight into the contribution of charged residues to other allosteri c properties , in this study we designed and synthesized a site directed mutan t , b82 Lys-Asn , for examing influencs the tetrameric stability and enthalpit ic change in O2 binding of human adult hemoglobin.

本研究焦点放在血红蛋白中央空腔中，带有正电残基的其中一个位置，b82 Lysyl ，来研究其正电离子效应与人类血红蛋白氧合反应热及四合体安定性的相关。针对这个目标，我们利用电脑做胺基酸序列排列比对和分子结构的模拟，选择以Asparaginyl残基取代Lysyl残基。

During the last decade, many studies have hypothesized that the MADS-box proteins could form specific dimers, which would be further assembled into tetrameric complexes to have more functions. In order to prove whether BCFMADS and CCFMADS genes can interact with each together to form either homodimer or heterodimer, we used an in vitro approach to analyze protein expressions for both genes.

MADS-box基因中，基因并不会单独的表现，而是透过形成同质二元体、异质二元体或是蛋白质复合体来表现其基因的功能性，因此本研究为欲证实BCFMADS以及CCFMADS基因间可否形成同质二元体或是异质二元体，乃利用E。

By O2 equilibrium, gel permission chromatography, and autooxidation analyses, we have investigated that the pos itively charge of b82 Lysyl residue is indeed involved in the exhothermic O2 b inding characteristics and somewhat stabilize the tetrameric heme moiety of Hb A.

其纯度以可见光谱和血色素电泳鉴定，并以氧合平衡曲线的测定，胶质层析和氧化速率的分析检视重组血红蛋白之性能。实验结果显示β82Lysyl残基所带的正电性确实影响血红蛋白氧合时的放热反应与其四合体的安定性。

Beta-thalassemia major is a serious hereditary hemolytic anemia which does great harm to human being.It is a monogenic disease characterised by a reduced synthesis of beta-globin chains,which dues to the Doint mutation or deletion of beta-globin gene and its control region.It not only decreases the hemoglobin level,but breaks the balance of alpha/beta synthesis ratio.The major cellular pathogenetic mechanisms in beta thalassemia are based primarily on the deleterious effects produced by the accumulation of the excess alpha globin chain.These excess alpha-globin chains cannot form stable tetrameric structure and deposit in erythroid cells.They cause large ineffective erythropoiesis on their own,accelerate red cells destruction,and lead to hemolysis.

地中海贫血是一种对人类健康危害严重的遗传性溶血性贫血病，是因β-珠蛋白基因及其调控序列的点突变或缺失致使β-珠蛋白肽链合成减少或完全停止，这不仅使患者血红蛋白水平降低，而且使原来在数量上与之持平的α-珠蛋白肽链相对过剩，这些相对过剩的游离α-珠蛋白肽链并不能形成稳定的四聚体，它们沉积在红细胞中导致了大量无效红细胞生成和红细胞寿命缩短，引起了严重的溶血性贫血。

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究，我们提出了初步的NodD操纵子激活模型：第一，四聚体是NodD蛋白的功能单位，它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子；第二，小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体，在我们的模型中，NodD四聚体缩小其V形主体的弯折角，进而缩短其DNA结合功能域的间距；第三，小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用，NodD的构象改变由蛋白质经其双铆钉位点传递给DNA；第四，只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物；第五，不排除NodD与RNA聚合酶可能有直接的相互接触位点，不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物，但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

Using the cationic detergent benzyldimethyl -alkylammonium chloride instead of cytochrome C in spreading procedure, we have observed the binding site for EcoR Ⅰ on linear pBR322 DNA molecule in the presence of gluteraldehyde. The diameter of the bound EcoR Ⅰ molecules are 15nm which may be the tetrameric EcoR Ⅰ particles.

用低分子量阳离子去垢剂苯二甲基苄基氯化胺取代经典展层技术中的细胞色素C，汲取前人经验，建立了无蛋白展层技术，适用于蛋白质--DNA相互作用的电镜研究。

Objective: Mannose-binding lectin is a plasma protein belonging to the family of collectins, composed of a N-terminal segment, helical coiled-coil hinge region, collagen-like stalk domain., and globular type C lectin domain or carbohydrate recognition domain. Full MBL biological function requires assembly to at least the tetrameric level.

目的：甘露聚糖结合凝集素(Mannose-binding lectin， MBL)是属于C型凝集素家庭胶原凝集素的一种血清蛋白，包括N末端区（N-terminal segment）、螺旋铰链区（helical coiled-coil hinge region）、胶原样区（collagen-like region，CRL）及球形C型凝集素区或糖识别区（carbohydrate recognition domain ，CRD）等4部分结构区域，四个亚单位的MBL形成有功能的多聚体。

This happens in a so called reaction center inPhotosystemⅡ, where a tetrameric chlorophylls (P680) absorb the solar light andinitiate photoinduced electron transfer reaction to the acceptors, quinone A and B. Thephoto-oxidized P680 retrieves the electron from a Mn4Ca cluster via a tyrosine, thelatter components are also called oxygen evolving center. After 4 photo-processes, twomolecular water are oxidized to a molecular oxygen.

在光系统二的活性中心，叶绿素P_(680吸收太阳光，将电子传递给电子受体、醌A和醌B，同时光氧化的P_(680)~+从释氧中心中得回电子(通过Tyr_z氧化Mn4Ca簇)，经过四次光诱导电子转移，两分子水最终被氧化生成一分子氧气。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。