查询词典 testis hormone

Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通过GenBank中的中华绒鳌蟹线粒体16S rDNA序列设计引物，利用PCR扩增方法，以beta-actin做内参，检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况，发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致，表明各模板DNA量基本一致；而在同一DNA浓度下，16S rDNA的扩增片段长度虽一致，但其产物量存在明显差异，精子16s rDNA产物量显著低于其他3种组织，其条带宽度和亮度很弱；16S rDNA扩增产物经测序分析及比对证明与已有序列完全吻合。

No DNA fragments were observed in electrophoresis in scrotal testis and cryptorchid testis on 3d.

而混合培养的生精细胞，在30min、1h可见明显的DNA ladder小片断，2h及12h则主要以坏死细胞造成的弥散小片断DNA为主。

Bcl-2 positive signals had no obviously changes in cryptorchid testis on 3d, but fluorescent signals of Bcl-2 protein decreased obviously in cryptorchid testis on 6-15d with the germ cell apoptosis increased.

随着隐睾诱导生精细胞凋亡增加，Bcl-2蛋白的表达明显下降，呈下调趋势；而Bax蛋白的表达明显增加，呈上调趋势，尤以精母细胞胞质更为显著。

To elucidate the relationship of GSE expression and gametogenesis, mRNA (0.5μg each) from mouse testis and ovary on different postnatal days were analyzed by RT-PCR with specific primers for GSE cDNA. The GSE mRNA in the testis of mouse was first detected at Day 14 postpartum, when cells at mid-pachytene are likely to appear.

为了阐明GSE表达与配子发生的关系，从0～49日龄小鼠睾丸和卵巢中提取mRNA（每样品0.5μg，将其与特定的GSE cDNA引物进行RT-PCR反应，分析的结果发现，GSE mRNA在出生后第14天的小鼠睾丸中首先被检测到，这一时间正是中粗线期精母细胞很可能出现的时期。

The results show:(1) In testis, PNA receptors sites were firstly observed in the cytoplasm and plasma membrane of primary spermatocyte, and greatly increased in the plasma membrane of second spermatocyte, then decreased and were accumulated in the tail of the round spermatid. The same reaction was observed in sperms of testis and spermatheca, which shows stronger reaction in head and weaker in tail. A universal dyeing was observed on the plasma membrane of sertoli cells.

结果表明：(1)在中华蚱蜢的精子发生中，PNA受体是由初级精母细胞合成，然后转移到膜上，在次级精母细胞，由于量的积累其质膜出现了强阳性标记；从早期精子细胞到成熟精子时期糖蛋白的分布发生了明显的修饰变化，这些变化与精子获得与卵子进行识别、粘附、结合等受精能力密切相关：受精囊内与精巢内的精子表面的标记是相同的，说明质膜糖蛋白的修饰作用在精巢内就已完成。

There are two ways: one is to use different size wooden model of the testis compared with the testis.

测量睾丸体积有两种方法：一种是用木制的不同体积的睾丸模型与睾丸比较。

To establish a model of piglet testis tissue cultured in vitro,the testis's tissues of 30-day-old piglets were used to observe in vitro spermatogenesis,and the differentiation of androgone was identified pre- and post-culture by transmission electron microscopy and HE.

以30日龄仔猪睾丸组织块为培养对象，应用飞利普TECNAI10电子显微镜对培养前后的生精细胞类别进行了观察，对培养后的组织块进行生精细胞分化程度鉴定，建立了仔猪睾丸组织生精细胞爬片体外培养模型。

The system was able to help androgone in testis tissue go through meiosis in vitro without addition of testos-terone by providing necessary conditions for differentiation of androgone.Key words: testis;tissue culture;in vitro spermatogenesis;transmission electron microscopy;HE staining

证实，在没有添加睾酮的情况下，此培养体系能够提供睾丸组织块生精细胞分化所需要的条件，使非精子细胞类生精细胞减数分裂为精子细胞。

Cancer-Testis antigens are specifically expressed in various types of human tumors but not in normal tissues except for testis. Among the CT antigens, NY-ESO-1 is the most immunogenically identified so far.

肿瘤免疫治疗，由于其具有抑制肿瘤细胞生长、杀灭残存肿瘤细胞和微转移灶的功效，因此辅助手术和化疗进行的肿瘤免疫治疗有可能成为肿瘤治疗中一个新的热点。

Despite evidence implicating prostaglandins as critical factor in male reproductive functions, little is known about expression and function of COX-2 in the male reproductive system. By using immunohistochemistry, RT-PCR and Western-blot, the content of this research included:(1) The expression and localization of COX-2 in mature rat and human testis were examined.(2) We investigated the changes of spermatogenesis and testosterone levels in testis after by using the COX-2 specific inhibitor-rofecoxib at 2, 4 and 8 weeks.(3) To explore the relationship between the intratesticular level of testosterone and expression of COX-2 by completely destroying the Leydig cells of mature male rats with injection of a single I.

本课题应用免疫组织化学、RT-PCR和Western-blot等方法检测以下内容：(1)在mRNA和蛋白水平检测正常成年男性及大鼠睾丸组织中COX-2的表达及定位情况；(2)实验组大鼠持续给予COX-2酶的特异性抑制剂Rofecoxib，分别于用药后2周、4周和8周检测大鼠的精子生成以及睾丸组织内睾酮浓度的变化；(3)另一组实验大鼠给予特异性Leydig细胞灭活剂EDS，在特定时间内抑制睾丸组织内合成的睾酮，于用药后2周和4周，采用免疫组织化学及RT-PCR方法观察大鼠睾丸组织内睾酮浓度变化对COX-2合成的影响。

Putt your way through 36 fun-filled holes of minigolf on 3D designed courses with elevated greens, bunkers, bridges and water hazards, among other crazy obstacles.

您的推杆方式，通过36个有趣的填孔迷你的三维设计的课程，以提升绿党，掩体，桥梁和水的危害，除其他疯狂的障碍。

Some participles can be used either as attributes or as predicatives.

有些分词既可当定语用，也可当表语用。