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It is recommended that armatures are tested using an armatures are tested using an armature analyzer.

建议测试电枢使用电枢分析仪。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus,FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗,本研究通过定点突变方法和PCR扩增方法,获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D,将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接,分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D;对重组质粒进行序列测定、分析,并将重组质粒分别转染BHK-21细胞,通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达,用电子显微镜观察病毒空衣壳的组装;为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果,将重组质粒经肌肉注射方法接种豚鼠,并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫,第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株;随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛,三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus,FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲,本研究通过定点突变方法和PCR扩增方法,获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D,将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接,分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D;对重组质粒进行序列测定、分析,并将重组质粒分别转染BHK-21细胞,通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达,用电子显微镜观察病毒空衣壳的组装;为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果,将重组质粒经肌肉注射方法接种豚鼠,并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫,第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株:随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛,三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

In this paper, we tested moisture adsorption capability of desiccants that were made from corn hull in different humidity and different temperature;we tested reversible adsorption of these desiccants too. The change of appearance of sample grain was validated by SEM and the augment of surface area was validated by mensuration.

在本研究中,对用上述方法得到的玉米渣皮干燥剂的吸水性能进行了测试,测试了干燥剂在不同相对湿度下的吸水率,干燥剂吸水率对温度的稳定性以及干燥剂的逆吸附性质,并通过扫描电镜测试和比表面积的测定验证了在处理后产物颗粒外观的变化和比表面积的增大。

Method] With 3 kinds of representative local duckweed varieties selected from the each sample place in Yangzhou City and Kunshan City resp. as the tested materials and the anaerobic wastewater diluted at 5 times and 10 times from the cattle Farm as the tested sewage,10 groups of purification treatments matching with 5 different duckweeds on 2 concn. of sewage were set up to investigate the effect of different duckweed variety on the purification effect of N and P in sewage with different concn.

方法] 从扬州市和昆山市各采样点选取的当地3种有代表性的浮萍品种为供试材料,以经稀释5倍、10倍的牛场厌氧废水为供试污水,设置5种不同浮萍对2种不同浓度污水的10组净化处理,在实验室条件下考察了不同浮萍品种对不同浓度污水氮和磷的净化效果。

According to the calibration information, Research and development status of automotive bus network and automotive calibration system is studied and the research significance is generalized. First of all, the collectivity precept for calibration system is designed, the calibration platform is set up based on the XCP Protocol and the calibration object is CAN bus network simulation ECUs. Then how to create the calibration system based on Ethernet and Series communication is expatiated, the communication way from computer to ECUs is introduced and the calibration system is tested. Then how to create the calibration system based on CAN bus communication is expatiated, the design and the interface programme are introduced and the calibration system is tested. At last the platform is used to calibrate the CAN bus network simulation system to regulate the simulation system's topology and communication speed to test the communication protocol's feasibility.

论文首先设计了标定系统的总体方案,以CAN总线实时仿真系统中的节点标定为研究对象,采用XCP协议建立具有串口、以太网和CAN总线三种通讯接口的标定平台;然后,具体开发了基于以太网通讯和基于串口通讯的标定系统,完成了上、下位机部分的软件设计,确定了标定系统在两种通讯方式下的数据处理方法,并分别应用两种标定方式标定下位机仿真系统的参数,让下位机仿真系统在规定的通讯协议和拓扑结构下进行仿真,对所设计的标定系统进行了调试;接着,设计了基于CAN总线通讯的标定系统,完成了CCP协议(&XCP on CAN&)的接口程序设计,并通过混合动力汽车一种拓扑结构的通讯仿真对所设计的系统进行了调试;最后,应用开发的标定系统,对混合动力电动汽车网络通讯协议进行了仿真研究,并根据实验结果进行了分析。

BCBBs are designed for not only symmetric linear arrays but also asymmetric linear arrays, are arrays and circle arrays. The BCBBs designed are tested by LFM signals, and the results show that the BCBBs achieve good quality. The word length of the filter efficients are studied from the view of engineering implementation. It is shown that using 8 bits can satisfy the requirement. Multi-beam BCBBs with 2 degree beamwidth in two octave and 1/3 octave frequency band are designed and tested for a specified array.

设计好的宽带恒定束宽波束形成器用线性调频信号进行了检验,证明了其正确性;从工程实际的角度出发,研究了滤波器系数字长对系统性能的影响,研究表明采用8位的滤波器可以满足要求;结合实际课题,在深入分析阵形结构特征的基础上,分别设计了倍频程和1/3倍频程多波束宽带恒定束宽波束形成器,并通过线性调频信号进行了验证,为其工程实现打下了良好的基础。

The length distribution of sliver fiber under conditions of different feed plate gauge was tested by USTER AFIS and fiber length distribution in taker-in droppings and card strips was tested by cotton fiber length analyzer.

用USTER AFIS单纤维测试系统,对不同给棉板与刺辊隔距条件下的生条纤维长度分布进行检测,用罗拉式纤维长度测量仪对不同给棉板与刺辊隔距的刺辊落棉、盖板花中的纤维长度分布进行了检测。

Study on fiber length distribution of taker-in droppings and card strips;2. The length distribution of sliver fiber under conditions of different feed plate gauge was tested by USTER AFIS and fiber length distribution in taker-in droppings and card strips was tested by cotton fiber length analyzer.

用USTER AFIS单纤维测试系统,对不同给棉板与刺辊隔距条件下的生条纤维长度分布进行检测,用罗拉式纤维长度测量仪对不同给棉板与刺辊隔距的刺辊落棉、盖板花中的纤维长度分布进行了检测。

Cast iron flat feet can be tested with the right-angle-foot right-angle ruler can not be tested using only two parts of the verticality of the gauge, cast iron flat foot also applies to equipment installation and inspection.

铸铁平尺可以配合直角尺检验只用直角尺无法检验的两机件的垂直度的量具,铸铁平尺还适用于设备安装和检查。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?