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BACKGROUND: The carboxyl terminuses of the Hsc70-interacting protein associate with many proteins in neurodegenerative diseases, which can promote the degradation of these proteins. Therefore, it is of a great significance to study the function of CHIP.

背景：热休克蛋白70羧基末端相互作用蛋白与许多神经退行性疾病的相关蛋白质存在相互作用，并促进这些蛋白质的降解，因此对热休克蛋白70羧基末端相互作用蛋白功能的研究具有非常重要的意义。

But an increasing number of people have been complaining of overcrowded trains, stations, airports, buses and terminuses and tourist spots during the Golden Weeks.

但越来越多的人开始抱怨黄金周期间的火车，车站，机场，公交车和站台，以及景区内都过于拥挤。

Including streets, squares, cinemas and bus terminuses and found thatthe taxis.

结果发现通过给出租车发送袋子后人们随地吐痰的频率有所降低。

The NS5B protein's RdRp activity is distinct when use different genetic-coming 3'terminuses of negative RNA strand as templates.

NS5B蛋白对HCV 1a、1b和2a型负链3'末端RNA模板具有不同强弱的聚合酶活性。

API-B has been successfully expressed in both yeast and E. coli system, and the expression products have been purified to homogeneity. By the method of deletion mutagenesis, it was proved that the intact N- and C- terminuses of APIB were necessary for the inhibitory activity, consistent with the tertiary structures of the inhibitors from the STl superfamily.

成功地建立了API-B在酵母及大肠杆菌体系中的表达方法和纯化路线，并通过缺失突变的研究，证明API-B的N-端及C-端附近序列对于维持分子的高级结构和抑制活性非常重要，该结果与STI抑制剂超家族成员的结构特征相一致。

To address this issue, we investigated the RNA synthesis in vitro from RNA templates corresponding to the 3\' terminuses of HCV positive- and negative-strand RNA by a purified HCV NS5B.

本论文利用纯化的HCV NS5B蛋白以及HCV正链、负链3'末端RNA，在体外条件下研究NS5B利用两种模板进行RNA合成的情况，以探讨HCV基因组模板选择性的机制。

They accuse the government of trying to ram the bill through without considering cheaper alternatives, and say the blueprint places the two rail terminuses at inconvenient locations, displacing a rural village near Hong Kong's northern border.

他们指责说，政府在没有考虑成本更低的替代方案的情况下，就想匆匆通过这项法案。他们还表示，规划图把两个终点站都放在了不方便的位置，并要拆迁香港北部边界附近的一个村落。

OBJECTIVE : to analyse the replication activity of NS5B proteins of HCV genotype 1a and 1b when use 3'terminuses of negative RNA strand from HCV 1a, 1b and 2a as templates by performing RdRp assay in vitro and offer some useful data to optimize the model for the screening of anti-HCV drugs targeting at NS5B.

目的：通过体外的RNA依赖的RNA聚合反应，分别比较HCV 1a和1b型来源的NS5B蛋白以不同HCV基因型负链3'末端RNA为模板时的复制活性，为优化以NS5B为靶点的抗HCV药物筛选模型提供理论依据。

The DNA sequences corresponding to the 3'terminuses of HCV genotype 1a, 1b and 2a negative RNA strand were cloned to the transcription plasmid pGEM3Zf.

以1a型负链3'末端RNA为模板，NS5B1和NS5B2为聚合酶，进行体外的RNA合成反应，斑点杂交和RT-PCR检测产物，确定纯化的蛋白具有RdRp活性。

The two NS5B proteins showed the same RdRp activity changes to these three templates: they had the highest activity using 3'terminuses from HCV genotype 2a negative RNA strand as template and the lowest activity using 3'terminuses of HCV genotype 1b negative RNA strand as template.

两种NS5B蛋白对HCV 1a、1b和2a型负链3'末端RNA模板表现出相同的活性差异，两种蛋白均对2a型负链3'末端RNA模板表现出最高的聚合酶活性，对1b型负链3'末端RNA模板表现出最低的酶活性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。