查询词典 terminus

Recombinant hK5 with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification.

经肠激酶切割和纯化，获得了与天然蛋白N-末端相同的hK5重组蛋白。

After thermal induction, no specific recombinant protein band in SDS-PAGE was found, but G-CSF activity was detectable. Therefore, a new recombinant plasmid pBVHG2 expressing hG-CSF hybrid protein with additional 8 amino acids which could be cut off specifically with the help of mucosal enterokinase at the N terminus of hG-CSF was constructed.

含此质粒菌株虽然表达菌体裂解后可测得明显的生物学活性，但SDS-PAGE仍未见特异表达产物带；因此，再应用相同方法，在hG-CSF cDNA突变体5′端增加24核苷酸对的FLAG肽编码序列，构建了hG-CSF杂交蛋白（hG-CSF天然蛋白N末端增加8aa的FLAG肽，后者可由肠激肽酶切除）的表达质粒pBVHG2。

We can not find any known domains in BEX1, but we notice that the C-terminus of it is similar to p75NTR-associated cell death executer, which is also highly expressed in brain and X-link. It may be a signaling adaptor molecule involved in p75NTR-apoptosis induced by nerve growth factor.

已有文献报道，在小鼠植入前的胚胎发育过程中Bex1基因在转录水平表达量发生变化，在出生后睾丸的发育过程中表达也有差异，说明这个基因与小鼠的发育相关。

Next stop is Ferry Pier terminus. All passengers please alight at this stop.

下一站系屯门码头总站，所有乘客请在此站落车。

2 STR-PCR We analysed the results of STR with the silver dye in the beginning. The silver dye of STR analysis on a sporadic DMD family shows the band confirmation is subjective by eyeballing. It can not detect the small repeats and the size of polymorphic fragments. For limitation of the silver dye, we analysed the band size of STR with the SAGAGT Genotyping System. Primer are designed according to the nucleotide sequence of five STR markers (located in 5' terminus and intron 44, 45, 49, 50) of dystrophin gene. The 5' end of the forward primer are attached a M13 tailed primer which conjugated to the infrared flourescent dye.

根据dystrophin基因5个多态位点（44、45、49和50号内含子以及5'端启动子区的序列）设计引物，在这些STR的上游引物5'端加一可被红外线荧光物质IRDye700识别标记的M13fw的尾：CAC GACGTT GTA AAA CGA C 通过PCR扩增出重复序列本身，经高分辨率的变性聚丙烯酰胺凝胶电泳后，利用红外激光遗传分析系统及SAGA〓基因分型软件对凝胶上的扩增产物及内标的位置，进行扫描分析，确定产物片段的大小。

March 18, Lijiang Airlines Southwest Airlines received a standing forest ranger terminus of transfer machines after the station Lijiang Lijiang dispatching room immediately to the airport for civil aviation flight plan to declare an emergency and inform the K-32A units.

3月18日，丽江航空护林站在接到西南航空护林总站的调机计划后，丽江站调度室立即向丽江民航机场申报紧急飞行计划，并通报K-32A机组。

Today is a starting point foy my dream, although I don't knew where is the terminus.

今天对于我是个起点，虽然我不清楚终点在那里。

As to Lucy Stewart, she quietly confessed to her origin and of her own accord spoke of her childhood and of the days when her father, the wheel greaser at the Northern Railway Terminus, used to treat her to an apple puff on Sundays.

有硕大的桃子，有这么大的葡萄，还有这样的季节里罕见的东西……在水果中间，放了六张一千法郎的钞票……这是那个俄国人……当然啦，我都退还给他了。不过，那一篮水果，我心里倒有些舍不得！

Arrives at terminus truly time, all already were unnecessary, like, once, to fondly remember, the recollection ......Is elapses;This thinks is hates, hatefully from where?

真正到达尽头的时候，一切都已经多余，爱、曾经、怀念、回忆……都是逝去；本以为是恨，可恨来自何处？

American Soybean Association and the Heilongjiang Fisheries Technology Extension Station and the Chinese National Aquatic technical cooperation to promote the terminus of the herring pond demonstration test.

美国大豆协会与黑龙江省水产技术推广站和中国全国水产技术推广总站合作开展了池塘养殖青鱼的示范试验。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。