查询词典 terminating chain

Using real-time reerse transcriptase polymerase chain reaction, transforming growth factor- messenger ribonucleic acid expression was quantitatiely measured.

通过实时逆转录聚合酶链反应，定量测定转化生长因子信使核糖核酸的表达。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Publicity, in Mr Weinberg's mind, was excellent as an enterprise—he was on the board of Knight Ridder, a large American newspaper chain, and helped orchestrate General Electric's purchase of NBC, a television network—but for his own business he thought it toxic.

公众的注意力，在温伯格的眼里，作为一项事业，当然很是精彩——他曾经是美国报业连锁巨头Knight Ridder的董事会成员，主导了通用电气收购美国国家广播公司的项目——但至于对于他个人而言，却是有害的。

For surfacing welding the wear out parts of agriculture and architecture mechanic, as grand bulldozer, roll-wheel of dynamic spade and ringy chain of car.

HRC≥50 用于农业、建筑机械等的磨损部分的堆焊，如大型推土机、动力铲的滚轮、汽车环链等。

They six areas are: Lea Valley; Epping Forest and River Roading; Thames Chase, Beam and Ingrebourne; London Riverside; Bexley River Cray and Southern Marshes; Green Chain Plus.

他们的6个领域是： lea的河谷； epping森林和河流巷道；泰晤士河大通，梁和ingrebourne ；伦敦河滨； bexley河克雷和南部沼泽；绿色连锁加。

Inhibition of PKCdelta by rottlerin or knockdown of TG2 protein by a TG2-specific siRNA resulted in a marked increase in autophagy shown by presence of autophagic vacuoles in the cytoplasm, formation of the acidic vesicular organelles, membrane association of microtubule-associated protein 1 light chain 3 (LC3) with autophagosomes, and a marked induction of LC3-II protein, important hallmarks of autophagy, and by electron microscopy.

抑制PKCdelta的rottlerin或击倒的TG2蛋白质的TG2特定的siRNA导致显着增加，表明自噬存在自泡在细胞质，形成酸性囊泡细胞，膜协会微管相关蛋白1轻链3（ LC3 ）与autophagosomes ，并显着诱导LC3 -Ⅱ蛋白的重要标志吞噬，并通过电子显微镜。

Dietary amino acid influences both fermentation and microbial community. Branch chain AAs are essential AA for the growth of ruminal mixed microbes.

底物氨基酸对瘤胃混合微生物群系及其发酵有一定的影响作用，支链氨基酸是瘤胃混合微生物生长所必需的氨基酸。

Guangzhou City Environmental Protection Bureau Chief Engineer Salicaceae told reporters that a high content of organic milk, the COD (chemical oxygen demand, detect water pollution indicators) amounted to 3,000 mg / l, while the untreated wastewater COD paper only 1000 mg / l; addition, as the issue of dumping milk containing melamine,"It's a question of the food chain as far as possible the post-diluted melamine content is low, almost not detected, but based on people's health is a highly responsible attitude In order to avoid the problem of milk contaminated fish the river."

广州市环保局总工程师杨柳告诉记者，牛奶中有机物含量很高，其COD(化学需氧量，检测水体污染程度的指标)达3000毫克/升，而未经处理的造纸废水的COD只有1000毫克/升；此外，由于倾倒的问题牛奶中含有三聚氰胺，&这有一个食物链的问题，尽量被稀释后的三聚氰胺含量很低，几乎检测不到，但本着对人民健康高度负责的态度，要避免问题牛奶污染河中的鱼类。&

Synthesis and characterization of tridentate chiral ligands, a series of Schiff-bases which are synthesized by salicylaldehyde, 2-pyridinecarboxaldehyde and amino alcohols derived from the amino acids without coordination group on side chain.

由侧链不带配位基团的天然氨基酸合成的氨基醇与水杨醛及2-吡啶醛缩合的Schiff-Base等三齿配体的合成与结构分析。

According to our present broadcasting situation,the thesis proposes using new technology to update and reform the present HFC. It also suggests broadcasting using self-advantage and arranging EPON in order to win the supermarket.

论文从我国广电的现状出发，结合实际，积极倡导采用新技术更新、改造我国现有的HFC网络；倡导广电现阶段利用自身优势，部署EPON网络来赢取市场。

A new general kinetic equation was derived in which the effect of the removal of condensation water was taken into account completely, and was applied to the polyesterification kinetic study of AA/HPHP and AA/NPG. It was found that the reaction order of self-catalyzed polyesterification was not constant. For these polyesterifications, the reaction order was 2.5 at a low temperature, while 3.0 at a high temperature.

考虑缩合水的排除对反应体系的影响，用体积浓度单位推导出一个新的聚酯反应总包动力学方程，并应用到AA/HPHP和AA/NPG两个聚酯化反应的动力学研究中，研究发现，自催化聚酯反应的反应级数并不是固定值，对于AA/HPHP和AA/NPG两个聚酯化反应而言，低温时反应级数为2.5，高温时反应级数为3.0。