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GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株,经筛选后倒置荧光显微镜观察细胞形态及荧光状态,挑取荧光最强的克隆,命名为ES-GFP细胞,进一步扩增培养,观察细胞生长及荧光表达情况,行细胞分化全能性鉴定包括碱性磷酸酶(alkine Phosphtase,AKP)染色、体外胚胎体(embryonic body,EB)形成实验及裸鼠体内成瘤实验。2。

There were 36 males and 40 females,with the average age of 47.7 years.36 patients presented with sciatica and perineal numbness,11 patients had painless mass and the other 20 were identified due to other causes.Among 67 benign neurogenic tumors,54 were originated from S1-S3 nerves,3 from S4-S5 nerves and 11 from the presacral space without caudal involvement.Giant cell tumor was found in 60 patients,which included 24 males and 36 females,with an average age of 32 years.Upper sacrum(S1-S2) was involved firstly.There were 56 patients(average,37.7 years) diagnosed as other primary benign bone tumors,which included 24 cases of teratomas,epidermoid cyst or dermoid cyst.Other malignant tumors,including chondrosarcoma (17),Ewing′s sarcoma/PNET(14),multiple myeloma(12),lymphoma(6) and osteosarcoma(6) were found in 64 patients.

其中脊索瘤95例,男62例,女33例,平均年龄55.7岁,骶尾区疼痛是主要症状(82例),半数以上患者伴有坐骨神经痛,肿瘤多先累及低位骶骨(S3~S5);神经源性肿瘤76例,男36例,女40例,平均年龄47.7岁,神经纤维瘤43例,神经鞘瘤24例,恶性神经鞘瘤9例,主诉多为坐骨神经痛、会阴部麻木(36例)和无痛性包块(11例),20例患者为查体或其他原因检查时发现,67例骶骨良性神经源性肿瘤起源于S3以上神经者54例,起源于S3以下神经者3例,发生于骶前未累及骶管者10例;骨巨细胞瘤60例,男24例,女36例,平均年龄32岁,腰骶尾部不适(37例)、坐骨神经痛(21例)是主要的临床表现,肿瘤多先累及上位骶骨(S1~S2);骶骨其他原发良性肿瘤及瘤样病变56例,包括畸胎瘤、皮样囊肿、表皮样囊肿共24例;其他原发恶性肿瘤64例,包括软骨肉瘤17例,尤文肉瘤14例,多发性骨髓瘤12例,成骨肉瘤6例等。

Benign cystic teratomas are well-differentiated tumors composed of a variety of parenchymal cell types representative of more than one germ layer, usually all three. The most common locations are the gonads and sacrococcygeus.

成熟囊状畸胎瘤是一种良性且分化良好的肿瘤,由胚胎原始细胞分化而成,由多於一层胚层之组织而成;好发位置为性腺体、骶骨与尾骨部或纵隔腔,十分罕见於头颈部。

The sonographic features of typical seminoma, teratomas, epidermic cyst, interstitial cell tumor and malignant lymphoma were obvious.

超声检查可以对睾丸肿瘤作出初步的诊断和鉴别诊断,为进一步治疗方案的制定提供依据,是睾丸肿瘤的首选影像学检查方法。

Results:In the group,there were 17(26.2%)cases of embryonal carcinomas,13(20%) cases of teratomas,10(15.4%) cases of yolk sac tumors,4(4.6%) cases of choriocarcinomas and 22(33.8%) cases of m...

结果:65例NSGCT患者中,胚胎癌17例(26.2%),畸胎瘤13例(20%),卵黄囊瘤10例(15.4%),绒毛膜上皮癌3例(4.6%),混合性生殖细胞瘤22例(33.8%)。

The indistinguishable yolk sac tumors cell in teratomas can be found by AFP staining combined with SP1 staining.

AFP和SP1可检出畸胎瘤中不易察觉的卵黄囊癌成分。

Mature teratomas of benign tumors, and said, skin samples cyst is one of the most common ovarian tumor, ovarian tumors by 10%— 20%, germ cell tumors of 85%— 97%, 95% teratomas.

成熟畸胎瘤属良性肿瘤,又称皮样囊肿,是最常见的卵巢肿瘤,占卵巢肿瘤10%~20%,占生殖细胞肿瘤85%~97%,占畸胎瘤95%以上。

Objective To analyze the 16slice CT features of ovarian teratomas.Methods The CT findings of 35 cases pathologicallyproved ovarian teratomas were retrospectively analyzed.

回顾性分析经手术及病理证实的卵巢畸胎瘤35例,均行16层螺旋CT检查,并进行横断、冠状、矢状及多平面重建图像。

Objective To analyze the 16 slice CT features of ovarian teratomas.Methods The CT findings of 35 cases pathologically proved ovarian teratomas were retrospectively analyzed.

回顾性分析经手术及病理证实的卵巢畸胎瘤35例,均行16层螺旋CT检查,并进行横断、冠状、矢状及多平面重建图像。

Objective To evaluate the ovarian teratomas that misdiagnosis or missed diagnosis by sonography and discuss the ultrasound characteristics in the rare ovarian teratomas and reasons for misdiagnosis.

目的 分析超声误诊或漏诊的卵巢畸胎瘤,探讨少见组织类型畸胎瘤的超声表现及复杂盆腔条件下畸胎瘤的误诊、漏诊原因。

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