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In this research, we extracted the genomic DNA from the Buxus sinica var. parvifolia leaves by using the modified CTAB approach and then used as the template DNA. Base on the invariable quantity of TaqDNA, we att empted to establish and optimize ITS-PCR amplification system for Buxus sinica var. parvifolia by employing L9 (3^4) orthogonal design with four factors, i.e. template DNA, Mg(superscript 2+), dNTPs and primer.

本研究利用改良CTAB法从珍珠黄杨叶片中提取基因组DNA作为模板，在TaqDNA聚合酶量不变的基础上，利用正交设计L9(3^4)对4个因素（模板DNA、Mg(上标2+)、dNTP和引物）在3个水平上对珍珠黄杨ITS-PCR反应体系进行优化。

I cut my cardstock template out and punched a 1/4" hole in the center tube. I glued the template to the two plates using rubber cement and my 1/4" bit for alignment.

i砍掉我的卡片模板，并挥拳打1 1 / 4 &孔在中心管，我胶合板模板，以上述两个板块的使用橡皮水泥和我的1 / 4 &位对齐。

A first embodiment comprises a first common primer sequence, a first template homology region, a cleavable site, a second common primer sequence and a second template homology region.

第一个实施方案包含第一通用引物序列、第一模板同源性区、可切割位点、第二通用引物序列和第二模板同源性区。

Template-confined growth is a very powerful method for obtaining aligned 1D nanomaterials with adjustable diameter, length and morphology. Porous anodic aluminum oxide templates with different channel structures have been synthesized in our lab, with which the aligned CNT arrays, Y-branching and novel dendriform CNTs have been obtained. Based on the structural characterization of the products, a space-limited and self-catalytic growth mechanism has been proposed accordingly. Roughly speaking, the catalyst particles at the bottom of template channels initiate the nucleation of carbon species, which acts as the seed for subsequent CNT growth.

本文采用近年发展起来的模板控制生长法，以多孔氧化铝模板控制CVD生长的方法调控了碳纳米管有序阵列、γ-分叉及树枝状分叉结构，在对产物进行详尽结构表征和分析的基础上阐述了氧化铝模板控制生长碳纳米管的生长机制：孔道内催化剂仅在生长初始阶段催化碳物种的成核，起&种子&作用，随后模板孔道自身的催化及限域作用使碳纳米管紧贴着孔道内壁生长。

Therefore, only nondependent names are bound at the point of definition of a template, and dependent names are bound at the point of instantiation, when a template is actually used.

因此，只有nondependent名称的约束，对一个模板，依赖名称在模板时，实际使用实例，点约束的定义问题。&/页&

Rapid amplification of cDNA end:Retroverse transcription product of total RNA extracted from normal porcine tissue was used as the template,gene specific primers were designed and advantage 2 polymerase mix was used in PCR,of which using porcine genomic DNA as the template:forward primer was designed according to the acquired consensus region of human and pig FGL2 3′ sequences while reverse primer was designed from human FGL2 3′ end downstream sequence;TA cloning.

以猪正常小肠及心脏组织提取新鲜总RNA，反转录后作为模板，设计基因特异性引物，采用Advantage 2 聚合酶混合物进行PCR扩增；依据猪与人FGL2基因3′端已知同源序列设计PCR上游引物，以人FGL2基因3′末端序列设计下游引物，以猪基因组DNA为模板采用Advantage 2 聚合酶混合物进行PCR反应；PCR载体重组质粒DNA亚克隆扩增。

The available image is found by template matching in original bill image. After the elimination of noise by filter, the image substraction is used to detecting the existence of template in available image.

在原始票据图象上利用模板匹配先得到相应的有效图象，再运用差影技术对滤波去噪后的有效图象进行模板存在与否的检测。

The genomic DNA of Unio douglasiae, collected from Suya Lake, Henan Province, was used as template DNA to optimize the RAPD-PCR reaction conditions including the concentration of DNA template, random primer, dNTPs, Mg2+ and Taq DNA polymerase and the annealing temperature for thermal cycle.

以河南宿鸭湖圆顶珠蚌的基因组DNA为模板，对淡水蚌类RAPD反应体系中的模板DNA浓度、随机引物浓度、dNTPs浓度、Mg~(2+)浓度、Taq DNA聚合酶浓度和和热循环参数中的退火温度进行了优化。

The edge matching method determines the optimal displacement vector between two successive frames by matching of the target edge template and the current unoccluded edge,and the template is updated in real time.

2多子模板匹配的方法，文献[7]以匹配误差判定子模板是否被遮挡，然后用灰度相关匹配继续跟踪目标；文献[8]基于最大

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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了