查询词典 template

Methods: Unrepeated MRSA strains from clinical specimens were divided into two groups: C-MRSA and H-MRSA, We used Chelex 100 to get DNA template and amplified mecA gene by PCR. The DNA templates of MecA were used to RAPD(random amplified polymorphic DNA), then we got the RAPD-DNA fingerprint-maps, randomly sampled 20 strains from each group as study objects.

收集临床MRSA标本，将其分为社区获得性MRSA与医院获得性MRSA两组，用Chelex 100裂解法制备DNA模本，PCR扩增mecA基因，选择mecA基因的DNA模本，用RAPD技术扩增出各菌株的RAPD-DNA指纹图谱。

Hepatitis B virus is the smallest DNA virus which is a member of the viral family Hepadnavirida. It uses its DNA as template and the RNA polymerase II of the host cells to transcribe its viral transcripts. There are four major RNAs transcribed from HBV genome. All of the four major transcripts are unspliced, terminated at a common site, polyadenylated at the 3' terminal and capped at the 5' terminal.

B 型肝炎病毒属於肝病毒科，为目前已知最小的去氧核醣核酸病毒，利用其病毒的去氧核醣核酸以及宿主细胞内的核醣核酸聚合酶可转录出四种主要的核醣核酸产物，此四种主要的转录产物均未经剪接并终止於核心开放编阅序列的共同polyadenylation 讯号，且在其5'端均经过capping修饰。

Results: Both phosphorothioate-modified oligonuclotides and oligonucleotides complementary with telomerase UNA template inhibited telomerase activity. And application of the tow ODNs together increased the inhibition activity in upfold.

结果：硫代磷酸酯寡核甘酸和RNA模板反义ODNs都可以抑制端粒酶的活性，且可产生累积效应。

Glue the uprights to the board at the locations shown on the base template using hot glue.

胶的门柱董事会的位置上表现的基础上，采用模板热胶。

And the Ni nanorods were prepared by reduction of NiCl2 with hydrazine in the virgulate liquid crystals microemulsion as colloidal chemical template at 70℃.

并在70℃时，以棒状液晶结构的微乳液为反应模板，用水合肼为还原剂还原NiCl2，合成出棒状纳米Ni。

New option to allow you to specify a different "Module Wapper Template" on a per-module basis.

新的选件让你叙述一个不同的&模数 Wapper 型板&在一之上每一-模数基础。

Zymic was simply to provide free web page template for others to download, then added a free web space.

Zymic 原本纯粹提供免费的网页模板让别人下载，后来又加上了免费的网页空间。

The results showed that the optimum concentration of five important components i.e. Tag DNA polymerase, Mg2+, primer, template DNA and dNTPs in 25μL RAPD reaction system were 1.0u, 2.5mM, 20ng, 40ng and 0.2mM, respectively. The RAPD fingerprinting of five Japanese plum cultivars were established by using optimum reaction system with ten random primers.

结果表明：1、改良陈大明法是提取中国李叶片基因组DNA的适宜方法。2、以5′-GACCGCTTGT-3′为随机引物，建立了优化的中国李RAPD反应分析体系：25μL反应体系中，Taq酶、Mg~(2+)、引物、模板DNA和dNTPs 5种主要成分的适宜浓度或用量分别是：1.0u、2.5mmol·L~(-1)、20ng、40ng和0.2mmol·L~(-1)。

The ISSR fingerprinting of 18 Japanese plum cultivars and 12 plum species or variety were established by using optimum system with primer 5"-9T-3"and 5"-9C-3", respectively. The identification rate of plum cultivars and species or variety was 100%. The effects of five important components on reaction of SSR were studied with Prunus salicina cv. Meilili. The results showed that the optimum concentration of five important components i.e. Tag DNA polymerase, Mg +, single primer, template DNA and dNTPs in 25 μL reaction system of SSR were 1.5u, 2.0mM, 0.8 μ M, 30~40ng and 0.16~0.24mM, respectively. The dendrograms of these cultivars was contructed according the Jaccard coefficient of similarity.

利用该优化体系，以5′-_9T-3′为引物，构建了中国李18个品种的ISSR指纹图谱，该引物可将这些品种完全区别开来；以5′-_9C-3′为引物，构建了李属6类种质资源的ISSR指纹图谱，该引物区分率为100％。4、中国李品种适宜的SSR反应分析体系是：25μL SSR反应体系中，Taq DNA聚合酶、Mg~(2+)、每个引物、模板DNA和dNTPs等5种成分的适宜浓度分别是：1.5u、2.0mmol·L~(-1)、0.8μmol·L~(-1)、30～40ng和0.16～0.24mmol·L~(-1)。5、采用RAPD、ISSR和SSR等三种分子标记技术对中国李品种资源54份和2个欧洲李品种进行了遗传多样性分析，并以品种间的遗传相似系数构建了Jaccard聚类树状图。

In this study,Using extracted total DNA from duck plague virus vaccine strain infected Chicken embryo fibroblasts cells as template, the 1.0 kb TK gene nonessential for viral replication was amplified by polymerise chain reaction and cloned into T-easy vector to obtain pGTK.

本研究从感染鸡胚成纤维细胞的鸭瘟病毒疫苗毒株中提取了总DNA，并以此为模板，利用PCR技术扩增出病毒生长非必需的TK基因(约1.1kb)，将其克隆入pGEM-T easy载体获得载体pGTK。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。