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This dissertation brings forward and realizes the multilevel classifiable method which is based on characters coding. Above all, this method realizes the first-grade classification by extracting enough effective characters from characters and coding them, to the others which coundn t be recognized by the first-grade classification, the method will adopt the second-grade classification using template matching to recognize these characters.

本文提出并实现了基于特征编码的多级分类识别方法，通过给字符抽取足够多的有效的特征并给特征编码实现第一级分类，对于第一级分类后仍不能区分的字符，再进入第二级分类用模板匹配的方法最终达到区分的目的，这种方法的重点在第一级分类阶段。

To use Clicky, you need to sign-up to the service and insert two lines of HTML code to your blog or website template , as simple as that.

使用clicky ，您需要登录的后续服务，并插入两条线的HTML代码到您的博客或网站模板，就这麼简单。

To use Clicky, you need to sign-up to the service and insert two lines of HTML code to your blog or website template , as simple as that.

使用clicky ，您需要登录的后续服务，并插入两条线的HTML代码到您的博客或网站模板，就这么简单。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The Wave C++ preprocessor library is a Standards conformant implementation of the mandated C99/C++ preprocessor functionality packed behind a simple to use interface, which integrates well with the well known idioms of the Standard Template Library.

Wave C++预处理器库是一个符合标准的委托型C99/C++预处理器实现，其功能被包装在一个易于使用的接口之后，该接口与著名的标准模板库惯用法集成得很好。

The Wave C++ preprocessor library is a Standards conformant implementation of the mandated C99/C++ preprocessor functionality packed behind a simple to use interface, which integrates well with the well known idioms of the Standard Template Library.

Wave C++预处理器库是一个符合标准的委托型C99/C++预处理器实现，其功能被包装在一个易於使用的接口之后，该接口与著名的标准模板库惯用法集成得很好。

Many methods for making nanoscale materials were developed in the past few years, such as colloidal crystallization, monolayer deposition, multiplayer casting, molecular crosslinking, complementary interaction, patterned etch pits, template-mediated and DPN, most of these are complicated and costive.

在过去的几年中，合成纳米复合材料的方法已取得了极大的进展，如胶体结晶法、单层膜沉积法、多层膜浇铸法、分子交联法、互补反应法、图形刻蚀法、模板化学法、DPN法等，但是这些方法大多操作耗时、费力。

First, used the template examination law to pick up character point of specific character information got from the pretreatment of fingerprint picture; then, used using the crestline track technology to filter the false characteristic point; at last, used the improved partial vector pattern matching method to match fingerprint, and made a judge of whether it belonged to the same person.

首先是对指纹图像预处理得到的细节特征信息用模板检测法来提取特征点，然后用脊线跟踪技术滤除伪特征点，最后采用改进的局部向量点模式匹配法来对指纹进行匹配，判断是否同属一人。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。