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The role of the solvents played in the reaction process was explored, which showed that acetylacetone was the reductant while ethylene glycol was performed as a ligand of Cu〓 as well as a"soft template" for the anisotropic growth of CuCl.

对溶剂在反应过程中所起的作用进行了深入研究，发现乙酰丙酮为还原剂，乙二醇即作配体又是CuCl晶体各向异性生长的&软模板&。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

A method is proposed that is based on template match and Radon transform of calculating the affined factors in digital watermarking against geometrical distortion.

针对目前数字水印抗几何攻击方法，提出一种基于模板匹配和Radon变换联合应用的几何变换参数估计方法。

By the use of RACE PCR technique with RNA extracted from Lycoris radiata young leaves as template and primers specifically designed according to conservative sequences of Amaryllidaceae lectins, the full length cDNA of Lycoris radiata lectin was cloned.

用石蒜科植物凝集素基因的保守序列为引物，利用RACE PCR技术从石蒜幼嫩叶片中克隆出石蒜凝集素的全长cDNA 。

The glutaraldehyde was coupled with amido group of chitosan, and template protein-bovine hemoglobin was immobilized by aldehyde group.

用壳聚糖包裹的硅胶为载体，利用壳聚糖表面的氨基与戊二醛结合，在硅胶表面引入醛基，固定模板蛋白。

In this paper, a series of 4,5-dithiacrown ether annulated 1,3-dithiole-2-thiones 8a~8d were prepared via condensing 4,5-bis-1,3-dithiole-2-thione (7) with 1,ω-dimercapto polyglycol derivatives in high dilution. The obvious template effect was found during the synthesis of 8c-8d.

在高度稀释条件下，经4，5-二溴甲基-1，3-二硫杂环戊烯-2-硫酮(7)和多缩乙二醇的1，ω-二巯基衍生物的缩合反应，方便地合成了4，5-位带二硫杂冠醚残基的2-硫代-1，3-二硫杂环戊烯的衍生物8a~8d，中间体硫杂冠醚8c~8d的合成中有明显的&模板效应&。

In this paper, a series of 4,5-dithiacrown ether annulated 1,3-dithiole-2-thiones 8a~8d were prepared via condensing 4,5-bis-1,3-dithiole-2-thione (7) with 1,ω-dimercapto polyglycol derivatives in high dilution. The obvious template effect was found during the synthesis of 8c-8d.

在高度稀释条件下，经4，5-二溴甲基-1，3-二硫杂环戊烯-2-硫酮(7)和多缩乙二醇的1，ω-二巰基衍生物的缩合反应，方便地合成了4，5-位带二硫杂冠醚残基的2-硫代-1，3-二硫杂环戊烯的衍生物8a~8d，中间体硫杂冠醚8c~8d的合成中有明显的&模板效应&。

The celluloid of the archangelic language has a function which also keeps the lesser creation in synchronization with the higher template, like the focusing device on the projector in our analogy.

大天使语言的赛璐珞具有同样使较少的创造物同步具有更高级生命模板的功能，同理就像投影机上的聚焦装置。

The celluloid of the archangelic language has a function which also keeps the lesser creation in synchronization with the higher template, like the focusing device on the projector in our analogy.

大天使语言的赛璐珞具有同样使较少的创造物同步具有更高级生命模板的功能，同理就像投影机上的聚焦装置。你知道它的英文吗？

Atactic polystyrene of larger molecular weight and narrower molecular weight distribution is prepared compared with the bulk polymer obtained outside the template.

以二氧化硅胶体晶为模板制备了无机/有机聚合物有序杂化体和聚合物孔材料，研究了聚合物在亚微尺度受限状态下的形成和性质。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。