查询词典 tck

Based on data of teliospore germination of Tt2 on day 50 after incubated,the model of relationship between teliospore germination rates and temperature for Tt2 was constructed as Y =0.150 77exp-(0.029 49( X -4.957 6)2.These results of biological experiments will provide fundamental data for risk assessments of establishment of TCK in China.

根据分离菌Tt2不同温度下培养50 d时的萌发率，建立了萌发率与温度的模型为Y=0.150 8 exp[-0.02949( X -4.957 6)]2，此结果为TCK的风险评估提供了基础数据。

The models describing the rates of teliospore germination of TCK and soil relative moisture capacity were established based on the rates of teliospore germination from islolates Tt1 and Tt2 after 50 and 60 days' culture at different soil moistures. The results provided the fundamental data for risk assessment of TCK.

根据分离菌Tt1和Tt2在不同土壤湿度下培养50d和60d的冬孢子萌发率，建立了TCK冬孢子萌发率与土壤相对含水量的关系模型，此结果为TCK的风险评估提供了基础数据。

Result The detection limit of the two systems were 0.1fg, equal to 2.31×10^4 copies, which was 10^2-10^3 fold higher than conventional PCR. By the constructed detection systems, the TCK and Tilletia cariesTul could be distinguished. The teliospore and mycelium of TCK in the infected wheat plant tissue also could be identified accurately and rapidly.

建立的两套定量PCR检测体系的检测下限相当，可达到0.1fg，对应的拷贝数为2.31×10^4个，检测灵敏度比常规PCR高2~3个数量级，均可成功鉴别出TCK与小麦网腥黑穗病菌Tilletia caries (DC Tul， TCT，并可快速准确检测小麦矮腥黑穗菌冬孢子和检测罹病小麦植株体内的侵染菌丝体。

In this reseach, we designed specific primer pairs based on the species-specific DNA fragment of TCK (1322 bp) from telomere associate gene of TCK and successfully established the routine PCR and real-time PCR detection system for teliospore or mycelium of TCK, which will facilitate accurate detection and identification of TCK teliospore on seeds, soil, flower and feeds and infected mycelium inside of wheat tissue for development scientific strateges of desease risk assessment and management.

本研究以筛选到的1322bpTCK独有差异基因设计TCK特异性引物对，建立TCK常规和定量荧光PCR技术体系，并研制出快速检测试剂盒。其研究成果将有助于对种子、土壤、原粮加工副产品、饲料和废弃物上病菌冬孢子流向和存活力进行动态监测，为制定科学的病害风险管理策略和监控方案提供技术支撑。

The relationships between germination of the teliospores of Tilletia conroversa Kühn and soil moisture were studied and it was found that the teliospores of TCK could germinate when the soil mass moisture content reached between 1%-28%(relative moisture capacity 3.75%-100%). The suitable moisture for teliospore germination was between 10%-25% of mass moisture capacity or 17.85%-89.3% of relative moisture capacity. The optimal soil relative moisture capacity was between 65%-75%. There was no difference among most TCK isolates tested at the same suitable soil moisture.

通过土壤湿度对小麦矮腥黑穗病菌( Tilletiacontronversa Kühn，TCK)萌发率的影响试验研究表明，其冬孢子在土壤质量含水量为1%～28%(相对含水量3.57%～100%)范围内均可萌发，其适宜萌发的土壤质量含水量范围为10%～25%(相对含水量17.85%～89.3%)，最佳土壤相对含水量范围在65%～75%之间；不同分离物在相同土壤湿度培养下，多数分离物冬孢子的萌发率之间差异不显著。

The main results were as follows:①The genomic DNA extraction method from TCK/TCT teliospore was established, which include cell wall lysis of teliospore by alkali solution, destroying teliospore cell wall for releasing nucleic acid from cytoplasma membrain and elimination of the PCR inhibitors such as pigments or polyphenols component.

论文主要研究结果如下：①优化了碱裂解法破冬孢子外壁-CTAB破坏原生质体，释放核酸-微量DNA过滤柱去除色素、多酚等PCR抑制物质的TCK/TCT冬孢子提取方法，排除了冬孢子细胞壁难破DNA难以提取，三甲胺、色素等PCR抑制物质导致假阴性的难题，采用此方法提取的腥黑穗菌DNA完全适用于PCR扩增。

TCK Wire Rope Non-destructive Inspection System adopts the TCK unique technology of "weak magnetism",by means of quantitative inspection on loss rate of effective cross-sectional areal of actual bearing metal,arising from wire rope's internal or external breakages,abrasion,corrosion and fatigue and other defects by use of TCK wire rope defectoscope

TCK钢丝绳探伤仪采用本公司独创的&弱磁检测&技术，以国际公认的钢丝绳安全承载能力的校核原则为检测和判定依据，通过对在线钢丝绳内外部断丝、磨损、锈蚀、疲劳等各种损伤导致的实际承载金属有效截面积损失率的定量检测，正确评估被测钢丝绳的剩余承载能力和使用寿命，为用户提供符合标准和规范要求的安全使用与合理更新的科学依据，是有效预防钢丝绳断绳事故、合理降低钢丝绳用绳成本和科学提高钢丝绳运行效率的高科技保障。

Independent of strong magnetic material, the weight of TCK weak magnetic product is very light. Inspection unit of all TCK portable models are less than 2 kg.

TCK的弱磁检测技术不依赖强磁材料的强度，因而重量很轻，TCK便携式所有型号的探伤仪，检测体重量均小于2kg

To detection of Tilletia controversa Kühn sensitively and accurately, real-time PCR systems were developed. The species-specific primer pair CQUTCK4/CQUTCK5 and probe CQUP1 were designed based on a selected specific fragment (1322 bp) specific for TCK, and the SYBR Green I and TaqMan quantitative PCR detection systems were established with optimized reaction conditions.

建立荧光定量PCR体系以准确灵敏的鉴定小麦黑穗菌(Tilletia controversa Kühn， TCK)根据筛选的TCK独有差异基因片段(1322bp)设计特异性引物对CQUTCK4/CQUTCK5和TaqMan探针CQUP1，建立SYBR GreenⅠ荧光染料法和Taq Man水解探针法定量PCR检测体系，并对体系进行优化。

At present, TCK has developed a series of products including portable inspection system, wired remote-control system, and wireless remote-control system to meet different demands of the customers in mining, harbor, ropeway, petroleum, electric power, hoisting, elevator, cable stayed bridge, missilery base and other wire rope related area.

目前，公司已经系统开发完成TCK便携检测系统、TCK有线遥控遥测系统、TCK无线遥控遥测系统等各种适合不同用户要求的系列产品，并开始为矿山、港口、索道、石油、电力、起重机械、电梯、斜拉桥、导弹基地等重要钢丝绳应用领域提供服务。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。