查询词典 targeting

In vivo, composition E markedly protected mice from lethal challenge by heat-killed E. coli and heat-killed S. aureus. Conclusions:(1) Targeting on PGN, CpG DNA and lipid A, we successfully established the platform to screen anti-inflammatory traditional Chinese medicine using biosensor technology which is highly effective, objective and feasible.

该组分经MTT法测定对小鼠RAW264.7细胞活力无影响(≤400μg/ml)，经CCK-8法测定也对小鼠RAW264.7细胞活力无影响(≤800μg/ml)；对PGN、CpG DNA及LPS刺激小鼠RAW264.7细胞分泌TNF-α有显著的抑制作用，并呈现良好的剂量-效应关系；对致死剂量的热灭活大肠杆菌和热灭活金黄色葡萄球菌攻击小鼠有显著的保护作用。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

International military officials have said they believed they were targeting a group of 120 insurgents.

国际部队官员表示，他们的袭击目标是120名武装分子。

They also found that "CRISPR interference," as this phenomenon is known, involves the targeting of the incoming plasmid or virus DNA directly.

他们也发现了CRISPR干扰作用，正如被认识的一样，该作用包括间接将进入的质粒或者病毒DNA作为目标。

Early embryonic lethality associated with conventional gene targeting of KIF 1A gene has hindered our progress in analyzing their functions in the adult and aging stages of life.

用传统的方法作成的KIF1A剔除鼠在生后早期死亡，使我们有关其成年及老年期的研究工作受阻，而条件性基因剔除巧妙地解决了这个问题。

In addition to these basic components, the conditional gene targeting construct had two loxp placed in the same orientation flanking the essential functional part of KIF1A gene-an exon which contains a ATP-binding consensus P-loop of the motor domain. We also carried a number of important considerations that have to be taken into account to generate a successful design for a KIF 1A conditional null allele.

除了上述基本特点之外，条件性基因剔除更主要的特点是，插入了3个方向相同的loxp位点，其中两个夹住了KIF1A的一段基本功能单位—一个包含了KIF1A运动区中和ATP结合的P-loop的外显子部位。

Romney has lanched several attacks in recent days, targeting both Huckabee and Senator John McCain of *.

Romney最近几天散步了很多攻击广告，主要针对Huckabee以及亚利桑那州议员John McCain。

Methods The hairpin sequences of siRNAs targeting VR1 gene of rat were designed, and two pairs of oligonucleotide sequence were synthesized. The annealed oligonucleotide fragments were cloned into linearized pRNAT-U6.2/Lenti expression vector and identified by PCR and DNA sequencing.

设计靶向大鼠VR1基因的发夹状siRNA，合成两对互补的寡核苷酸序列，退火后克隆到酶切的pRNAT-U6.2/Lenti siRNA载体，通过PCR和DNA测序鉴定重组质粒。

By using lentivirus vector system, effects of targeting genes and their mechanisms will be investigated in vitro.

使用慢病毒载体系统，观察目的基因在体外实验中的具体作用及其可能的作用机制

OBJECTIVE: To find the valid small interference RNA targeting human BI-1 gene by Lentivirus.

目的：为了利用慢病毒Lentivirus介导的RNAi技术寻找抑制人BI-1基因表达的siRNA。

Salt is good, but if the salt becomes flat and tasteless, with what do you season it?

14：33 盐本是好的，盐若失了味，可用什么叫它再咸呢？

He reiterated that the PLA is an army of the people under the leadership of the Communist Party of China.

他重申，人民解放军是在中国共产党领导下的人民军队。