查询词典 targeting

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Targeting these sticky proteins with drugs is difficult, however, as plasmodia contain many different varieties, which they use to evade the human immune system.

但是，以这些粘性蛋白质作为药物靶标非常困难，这是由于疟原虫用于侵入人类免疫系统的蛋白质种类很多。

To study the biologicaleffect of pulsed electromagnetic fieldson the proliferation of Immunomagneticseparated human precartilaginous stem cellsin vitro.To establish immortalizedhuman precartilaginous stem cellsstrain and provided stable cell resource to studythe molecular mechanism of gene targeting on differentiation of PSCs.①The cells from the aborted fetus\'s metaphysis were digested by collagenase,the PSCs were isolated by magnetic-activated cell sorting,then were subculturedand amplified.

探讨体外分离、培养人前软骨干细胞的可行性，分析其表型特点；研究脉冲电磁场对免疫磁性细胞分选的人前软骨干细胞增殖的生物学影响；构建猿肾病毒40大T抗原基因(SV40Tag)介导的永生化人前软骨干细胞株，为下一步基因打靶研究其分化分子机制提供稳定的细胞来源。

Conditional rBDNF transgenic vector was successfully constructed and strong overexpression of rBDNF and EGFP were identified in the cotransfected cell line, and establishment of this targeting vector is critical for pronuclear microinjection.

rBDNF条件性基因载体构建成功，在体外细胞系中，检测到rBDNF及EGFP强表达，为转基因显微注射打下基础。

Three-dimensional targeting device was used to introduce the puncturation. The bone cement which was 0.2-0.5 mL less than the calculated volume was injected into osteolysis site.

然后使用自行设计的三维穿刺导向器引导穿刺，按照手术前计算的容积略少于计算量0.2~0.5 mL注射骨水泥到骨盆溶骨破坏部位，充填满意后退出穿刺针。

The SPECT imaging of ~(99)Tc~m-GEBP11 can show the tumor mass clearly and ~(131)I-GEBP11 has anti-tumor effect with weak toxicant and secondary effect, which is the foundation for them to develop nuclide probe or radiotherapeutics drugs targeting to tumor vasculature.3. GEBP11 can inhibit angiogenesis, which comes true probably through its inhibition effects on the proliferation, invasion, migration and adherence of ECs.

1、GEBP11具有活体内胃癌血管靶向结合的能力，它与胃癌血管内皮细胞膜受体特异结合后可被内化。2、~(99)Tc~m-GEBP11 SPECT成像能较好地显示体内肿瘤灶，~(131)I-GEBP11具有明显的抗肿瘤效应及较弱的毒副作用，为开发肿瘤血管成像核素探针及受体放射治疗新药奠定了基础。3、GEBP11具有抑制胃癌血管生成的作用，这可能通过抑制ECs增殖、基质降解及迁移、粘附能力实现。

Objective: Using phage display randem peptide library to generate peptides as small molecular weight vectors for tumor-targeting therapy.

目的：探索用噬菌体随机肽库筛选可用于肿瘤导向治疗的新型小分子载体。

Time is beginning to run out, however, as Democratic leaders are targeting Monday for the introduction of the FAA reauthorization bill.

时间开始慢慢流逝，然而，民主党领导人最终决定周一正式听取FAA授权议案的介绍。

The position of string nock point of recurve can directly affect the archery s dynamic equilibrium and arrow s swing range , its balance and targeting rate.

箭巢位置的确定将直接影响反曲弓的动态平衡稳定性和箭杆离弦后箭杆尾部的摆动幅度，并最终影响箭杆的飞行稳定性和射箭命中率。

S have to redetermine their marketing strategy and tactics which generally contrary to what they have done at their home markets based on their knowledge of the targeting countries' culture context.

而化学主要研究分子的特征和相互作用。相对于物体学主要从整体研究物质普遍属性，广义而言，化学集中研究各种元素和化合物的特定属性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！