查询词典 targeting dots

Maney contends the magic formula is a combination of targeting your market correctly and staying true to your goal.

梅尼争辩神奇的公式是市场定位的正确和真实留在你的目标相结合。

Because animal miRNAs bind with imperfect complementarity, miRNAs are though to be capable of targeting numerous mRNAs; thus, misexpression of one miRNA can disrupt the expression of hundreds of proteins.

因为动物的microRNA结合并不完全互补，所以它能与多种多样的mRNA结合，因此，一个microRNA的错误表达就会扰乱成千上万蛋白质的表达。

Results among the 104 primary acute leukemia patients, the number of patients with t lymphocyte leukemia,b lymphocyte leukemia,acute myeloblastic leukemia,mixed phenotype acute leukemia and umclassified leukemia were 4(3.8％),38(36.5％),58(55.8％),2(1.9％),and 2(1.9％),respectively.conclusion detecting immune marker on leukemic cell by abc-ap two step immune method is very simple which can be finished with a light microscope,and it can provide a reliable basis for the diagnosis and classification of leukemia and even the targeting therapy for leukemia.

结果 104例初发急性白血病中，t淋巴细胞白血病4例(3.8％)，b淋巴细胞白血病38例(36.5％)，急性髓细胞白血病58例(55.8％)，急性混合性白血病2例(1.9％)，未行明确分类的2例(1.9％)。结论 abc-ap免疫二步法检测白血病细胞免疫标记方法简单，只须普通光学显微镜就能开展实验，可为白血病的诊断、分型、分类乃至白血病靶向治疗提供可靠依据。

Methods Targeting the most common mutation types at codon 526 and codon 531 of Rifampiein Resistance Determining Region of rpoB gene from Mycobacterium tuberculosis ,labeled probes （526CAC, 526TAC, 531TCG and 531TTG） were designed to detect 38 Rifampin-resistant clinical isolates with known RRDR sequence, 24 Rifampin-sensitive clinical isolates with wild type sequence of RRDR and 5 nontuberculous mycobacteria isolates, then a method using real-time PCR was established.

方法针对结核分枝杆菌rpoB基因利福平耐药决定区（Rifampicin Resistance Determining Region，RRDR）526密码子和531密码子常见的突变形式设计探针（526CAC，526TAC，531TCG和531TTG），应用已知rpoB基因RRDR区序列的38株利福平耐药临床分离株和24株利福平敏感临床分离株以及5株非结核分枝杆菌菌株建立荧光定量PCR检测基因突变的方法。

For example, much of our prevention and treatment programs are based heavily on education. These programs often assume that if we can make the group we are targeting with our programs understand how terrible the outcomes of abusing drugs can be, their orbitofrontal cortexes will be strongly motivated

例如。我们主要的预防和治疗计画是透过教育，这些计画通常认为如果我们以此计画教导特定团体来了解药物滥用的严重后果时，他们的眼额叶皮质就会受到强烈动机影响，协助前额叶皮质执行它的功能，抑制药物滥用行为并作出有益的选择。

However, techniques to generate cell type-specific lineage reporters, as well as reliable tools to disrupt, repair or overexpress genes by gene targeting, are inefficient at best and thus are not routinely used.

然而，产生细胞类型特异的报告体，以及实现基因的干扰、修复和过表达的可靠的基因打靶工具的效率都不是最好，因此并没有被常规使用。

Petr Cech reveals on Chelsea TV tonight that he is targeting a mid-January return to full training.

切赫在今晚的切尔西电视节目中透露他争取在1月中旬恢复完整训练。

The inflammatory effects, pharmacodynamical basis, and multi-targeting properties of Xuebijing, a traditional Chinese medicine prescription, were investigated on molecular level.

研究了血必净抗炎作用的药效物质基础，并在分子层面上对复方多靶点作用进行阐释。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Targeting these sticky proteins with drugs is difficult, however, as plasmodia contain many different varieties, which they use to evade the human immune system.

但是，以这些粘性蛋白质作为药物靶标非常困难，这是由于疟原虫用于侵入人类免疫系统的蛋白质种类很多。

Salt is good, but if the salt becomes flat and tasteless, with what do you season it?

14：33 盐本是好的，盐若失了味，可用什么叫它再咸呢？

He reiterated that the PLA is an army of the people under the leadership of the Communist Party of China.

他重申，人民解放军是在中国共产党领导下的人民军队。