查询词典 targeted

The number of differentially expressed genes was 71 at 24h, and 62 at 72h. The metabolisic gene class was the majority among all the differentially expressed genes, and the next was transportational、differentiation and adhesional gene class by turns.1.2. RT-PCR verification of the known HIF-1 targeted genes and proliferation related genesIn the 131 differentially expressed genes, we found 7 known HIF-1 targed genes (ldha、aldoa、eno1、gapdh、tpi1、pgk1、bnip3) and 5 genes (dnaja2、ccnb1、tgfβ2、ccng1、rgs7) which were related to proliferation and cell cycle.

对差异表达的基因进行基因功能分类发现：代谢类基因最多，其次是运输类基因、细胞分化及黏附类基因。1.2 RT-PCR验证HIF-1靶基因和增殖相关的基因由于我们的前期实验证明了低氧促NSC增殖是有HIF-1参与的，我们对131个差异表达基因中，7个已知的HIF-1靶基因(ldha、aldoa、eno1、gapdh、tpi1、pgk1、bnip3)和5个与细胞周期和增殖相关的基因(dnaja2、ccnb1、tgfβ2、ccng1、rgs7)做RT-PCR验证。

Since the AES submission is targeted at w = 32 and r = 20

由于AES公司提交的对象是瓦特= 32和r = 20

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Ongoing projects involve targeted gene disruption, high-throughput approaches for functional exploitation of Plasmodium and Anopheline genomes and identification of molecular components of the recombination machinery in malaria parasites.

正在进行的项目包括有针对性的基因破坏，高通量的方法开发功能疟原虫和按蚊基因组和分子识别元件的重组机制的疟疾寄生虫。

In my opinion, one, the original antemarital examination system of our country there are serious deviations, thus violating the right of citizens of the conclusion of the marriage, the new system was rejected antemarital examination antemarital examination of the important medical value and ignores the marriage party's right to know, two, the lack of a single antemarital examination content targeted the lower end of the level of detection.

我认为，第一，我国的原有婚检制度存在严重偏差，侵犯了公民的婚姻缔结权，新的婚检制度又否决了婚检的重要医学价值并且忽略了结婚当事人的知情权。第二，婚检内容单一缺乏针对性，检测技术水平较低端。

Part Ⅲ: Animal immunization and anticarious experiment of the glucan binding protein B Plasmid pcDNA3. 1 -gbpB DNA vaccine was delivered into Sprague-Dawley rats in two different routes: intranasal and targeted salivary gland immunization . The dynamic variety of specific antibodies in sera and saliva were checked by ELISA.

第三部分 DNA防龋疫苗免疫动物及抑龋实验研究大量制各重组质粒pcDNA3.1-gbpB，分鼻腔滴注组、颌下腺区皮下注射组免疫SD大鼠，并设对照组和空白组，采用ELISA法检测血清及唾液中抗体水平的变化情况。

At the same time, the New York State Department of Health aired a separate, simultaneous statewide television-based antitobacco media campaign that also targeted New York City, with advertisements featuring graphic images of the effects of smoking and highlighting the effects of secondhand smoke on children. From January through December 2006, New York City adult smokers were exposed to nearly 11,000 GRPs as a result of both campaigns combined, equating to the average viewer in NYC seeing an advertisement approximately 110 times during the year.

同一时期，纽约州健康局则同步向全州收视户播出另一项反菸媒体运动，纽约市亦作为目标区域，它是以吸菸所造成之健康影响的图像，并强调二手菸对儿童的影响为内容的广告；2006年一整年，在两项运动总合的影响力下，纽约市成人瘾君子所接触到的反菸讯息有将近11，000 GRPs，相当於纽约市一般收视观众在一整年当中看同一则广告约110次。

Dr. Arya Sharma says being obese doesn't necessarily doom people to poor health and that weight loss recommendations should be targeted at those most at risk because of medical problems.

圣夏尔马博士说肥胖不一定能使人们不健康，而且体重减轻建议应针对那些处境最危险的人类，因为医疗的问题。

Army spokesman Major General Athar Abbas says recent operations have targeted militant training camps and ammunition dumps, killing more than 100 Taliban fighters, including some top commanders.

军方发言人阿巴斯少将说，最近战役的目标指向激进分子训练营和堆放弹药的地方，他们击毙了100多名塔利班武装分子，其中包括一些高级指挥员。

When the Republic transitioned to the Galactic Empire, Autem found himself at odds with the new government, and was targeted by bounty hunters dispatched by Darth Vader.

共和国演变为银河帝国后，奥特姆发现自己与新政府政见不合。达斯·维德派赏金猎人追捕他。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐