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A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising subjecting said sample to an amplification reaction using a set of nucleotides, at least one of which is fluorescently labelled, contacting amplification product with a probe under conditions in which the probe will hybridise to said target sequence, said probe comprising a reactive molecule which is able to absorb fluorescence from or donate fluorescent energy to said fluorescent labelled nucleotide and monitoring fluorescence of said sample.

一种用于检测在某样品中是否存在目标核酸序列的方法,该方法包括:使用一套核苷酸对所说的样品进行扩增反应,其中至少一个核苷酸是被荧光标记的,在探针与目标序列杂交的条件下使扩增产物与探针混合,所说的探针包括一种活性分子,该活性分子能够从荧光标记核苷酸吸收荧光或向其贡献荧光能量,及检测样品的荧光。

It is adopt a flexible and innovative pattern,exert the superiority of target language,interpret the idiomatic expression in accordance with target language on the basis of original brand meaning to left consumers a deep impression.

2意译法。意译法是商标翻译的另一种常见的方法,它是根据原商标的含义,采用灵活创新的方式,发挥目的语的优势,翻译符合目的语的表达习惯,给消费者留下深刻的印象。

Deals 429 to 497 Fire damage to your target and an additional 107 to 125 Fire damage if the target is affected by an Immolate spell.

对你的目标造成429至497点火焰伤害,如果目标带有献祭效果,则额外造成107至125点火焰伤害。

This paper took the high temperature rotation scanning target of the high-current oxygen ion implanter which was designed by the Forty-eighth Institute of CETC as the research object, present modeling and simulation of this target, by utilizing parameter feature modeling and virtual prototype technology.

本文以中国科技集团公司研制的大束流专用强流氧离子注入机的高温旋转扫描靶为研究对象,结合参数化特征建模技术和虚拟样机技术,对高温旋转扫描靶的建模和仿真做了探索性的研究。

From theory and simulations,it is found that,under some conditions,adjusting the included angle between the sensors and target can improve the target tracking accuracy.

通过理论证明和仿真得出,在一定的条件下,通过调整两传感器和目标所成的夹角可以大大地提高对目标的跟踪精度。

Firstly, This paper processes geometry filtration based on character of orbit's apogee and perigee after orbit model is analyzed and based. Secondly Space Debris is separated into two kinds, coplanar and not coplanar according as location interrelation of target and debris. In Dynamic filtration's processing, considering prediction time limit and orbit perturbation, secular effects which have increaser effect is considered so that some need catching danger time point is appeased, some law about time when target and debris pass intersectant line each other is inferred, when debris and object are in different plane and some similar law also is inferred otherwise. The time list is arithmetical progression. These laws can improve calculation efficiency remarkably; Lastly orbit predict is a course approaching least distance. Danger time point in the last step become beginning time point from which debris and object's position and velocity are calculated by SXP4 Model which is an analytic model.By result, determine that relative movement trend is closer or apart, farther calculate the interval apart from time when predictive least relative distance occurs. Least relative distance and time when least distance occurs is calculated finally. It is a fast approach course adopting varied interval.

本文在分析、建立轨道摄动计算模型的基础上,首先采用基于轨道远、近地点特征对大量编目在册的空间碎片进行初步几何静态筛选,然后根据碎片与目标轨道面的相对位置特点把碎片分为异面与准共面两种情况以进行动态筛选,在筛选过程中考虑到预报时限长度和轨道摄动影响,引入了轨道摄动中具有积累效应的长期项以满足危险时间点的捕捉要求,利用了长期项摄动变化线性的特点导出了碎片与目标异面情况下各自过交线的时间序列成准等差数列的规律性和准共面情况下类似的规律性,从而显著地提高了前期轨道筛选的计算效率;最后对剩余危险碎片和目标飞行器利用SXP4轨道预报模型在前期轨道筛选的基础上以各个危险探测时间点为预报的时间起点,并根据预报计算结果,分析目标和碎片在该时间点的运动趋势是相互远离还是相互接近,推出预计最小相对距离发生时刻相对此刻的时间长度,得到其随时间变化的局部最小相对距离以及危险距离发生的时刻和相对速度,这是一个采用变时间步长、快速逼近最小相对距离的过程。

The Chinese People are winning their way towards the great target of building an overall well-to-do society. And energy sources are an important indemnitor to actualize the target.

中国正在向全面建设小康社会的伟大目标而奋进,能源是实现这一目标的重要保障,但是我国在多年的发展过程中还存在许多能源问题,这些问题主要包括能源缺口问题、能源结构问题、能源分布问题、能源人均问题、能源效率问题以及能源消耗比重问题。

The difficult part of target tracking lies in the indetermination of target, including the maneuverability of targets and the character of multiple targets tracking.

目标跟踪的主要难点在于目标的不确定性,包括目标的机动性和多目标的问题。

If they give up their own culture in order to meet special requirement of target culture, they will be at the risk of being assimilated by target culture and thus becoming indistinctive.

如果他们在商品包装上遵从目标国的文化要求,弱化自己的文化属性,其自身包装文化必然有被目标国文化所同化的风险,从而变得毫无特点。

The removal pathways are: fist, high-energy electrons undergo an inelastic collision with target molecule, and transfers all or part of its kinetic energy to molecule. Inelastic collisions result in a charge to the target molecule such as ionization, dissociation or excitation.

其可能的途经是:首先,高能电子经过与目标分子发生非弹性碰撞,将能量全部或部分传递给分子,使其裂解,激化等;接着,上述被高能电子裂解、激化的分子在臭氧和氧等离子的作用下,被氧化成CO2、CO和H2O。

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推荐网络例句

"Yes, now you can give yourself airs," she said, you have got what you wanted.

"对了,您现在高兴了,"她说道,这是您所期待的。

Then the LORD said to me: Rebel Israel is inwardly more just than traitorous Judah.

上主于是对我说:"失节的以色列比失信的犹大,更显得正义。

I do my best to guide my congregation,ok?

我尽了全力引导会众明白吗?