查询词典 tap-water

The results showed that RSAP used two primers of 18 nucleotides in which starting at the 3' end of each primer were restriction site sequences (4-6 bases),followed 12-14 bases of arbitary sequence, the differences between two primers were restriction sites and filler sequence; PCR am-plification was run for the first 5 cycles with an annealing temperature of 35℃, followed by 35 cycles with an annealing temperature of 48℃; the PCR reaction of 25 μL included 20ng DNA templates, 2.5mmol/L of Mg(superscript 2+), 0.2 mmol/L of dNTP, 1.5U of Taq DNA polymerase, 600nmol/L of each primers. RSAP is a new maker technique with simplicity, moderate throughput ratio and reliability. RSAP is of good reprodicibity and broad application.

结果显示，RSAP技术的引物为2条长度均为18bp的引物，引物的3'端为4~6个碱基的限制性酶切位点序列，接着是12~14个碱基的随机序列，2条引物的限制性位点和随机序列不同：PCR扩增的前5个循环采用35℃的退火温度，随后的35个循环采用48℃的退火温度；在25μL反应体系中，模板DNA用量为20ng，Mg(上标 2+)浓度为2.5mmol/L dNIPs浓度为0.2 mmol/L，Tap DNA聚合酶用碳为1.5U，2条引物浓度均为600mmol/L；RSAP技术重复性好，适用性广泛，是一种操作简便、产率中等、稳定可靠的DNA标记技术。

American British apartment flat bathroom toilet, W.C., or loo candy sweets checkers draughts closet cupboard corn maize cracker biscuit diaper nappy drugstore chemist's faucet tap gas, gasoline petrol hood bonnet line queue napkin serviette oven cooker round-trip ticket return ticket suspenders braces truck lorry trunk boot underpass subway undershirt vest vacation holiday Compounding spelling and word choices, there are also punctuation differences between American and British English.

美国英国地公寓单位地浴室厕所，卫生间，或乃糖果甜食跳棋吃水衣柜橱柜玉米玉米饼干饼干尿布尿布药店药房水龙头水龙头天然气，汽油发动机罩线队列餐巾餐巾纸烤箱锅往返机票回程机票，吊带汽油发动机罩括号卡车货车干线启动隧道，地铁汗衫背心休假假期复合单词拼写跟选择，也有美国跟英国之间地英语标点符号地差异句号。

This led to the discovery of Shamanism and the urge to tap into my basic instincts.

这引出了对萨满教的发现和探究我内在本能的强烈欲望。

Actually, yes, people do kick their horses to get them moving, but it is more like a gentle tap of the heel against the horse's side than a true kick.

其实，是的，人都踢他们的马匹，让他们感动的，但更喜欢温柔塔的鞋跟对马的一侧比一个真正的踢。

Silva UltraMind teaches you to tap into your intuition to understand what your purpose in life is.

Silva UltraMind 教你进入你的直觉来理解你的目的是在生活中。

On inquiring further about him I learned that he was a universal favorite in the village, and the oracle of the tap- room; where he delighted the rustics with his songs(1), and, like Sindbad(2), astonished them with his stories of strange lands, and shipwrecks, and sea-fights.

经过进一步打听，我了解到他在村子里深受众人喜爱。他在酒店里是一位博古通今，未卜先知的智者。在那里，他的歌声使得乡里们笑逐颜开，他象《天方夜谭》里的辛巴德，用那些异乡仙境，沉船和海战的故事使他们听得目瞪口呆。

Brad Paisley -- Letter To Me 写给自己的信 If I could write a letter to me And send it back in time to myself at 17 First I'd prove it's me by saying look under your bed There's a Skoal can and a Playboy no one else would know you hid And then I'd say I know it's tough When you break up after seven months And yeah I know you really liked her and it just don't seem fair All I can say is pain like that is fast and it's rare And oh you got so much going for you going right But I know at 17 it's hard to see past Friday night She wasn't right for you And still you feel like there's a knife sticking out of your back And you're wondering if you'll survive You'll make it through this and you'll see You're still around to write this letter to me At the stop sign at Tomlinson and Eighth Always stop completely don't just tap your breaks And when you get a date with Bridgett make sure the tank is full On second thought forget it that one turns out kinda cool Each and every time you have a fight Just assume you're wrong and dad is right And you really should thank Mrs.

如果我能写封信，寄回给十七岁时的自己。那里有一个啤酒罐和一本"花花公子"，没人知道你把它们藏在那儿。然后我要说那很令你难受，当你交往了七个月的女孩儿与你分手，是啊我知道你好喜欢她，那似乎不很公平，但我只能说那是短暂的痛苦而且也极少发生。哦，你有着美好的未来，但我知道十七岁时很少有谁人去想周五晚上以后的事儿。她不适合你，你却依然心如刀绞，不知能否活下去。但最终你能熬过这一切而且你会看到你仍然好好的并写这封信给我。在Tomlinson与第八街的交口处，停车信号时一定要将车停住而不仅仅是点一脚刹车。当你和Bridgett约会要确信油箱是加满的，再仔细想想，别太在意了，那个女孩儿并不很酷。每一次的争执，就只当你是错了而爸爸是对的。而且你真要好好感谢Brinkman老师，她为你花费了那么多额外的时间，好像她看到了地下的钻石，她不停地打磨直到你闪闪发光。

The current key to be pressed will be highlighted and a warning sound will play if you tap the wrong key.

当前重点要压将得到突出，并发出警报声，将起到如果你踏错了钥匙。

A great search. With Solr, the leading open source search server, you'll tap into a host of features from highlighting search results to spell-checking to faceting.

利用Solr这个领先的开源搜索服务器，你将深入了解一套拥有突出显示搜索结果、语法检查以及分面搜索的系统。

And now Bear, you can tap the spigot into place.

现在该你了，小熊，你可以把龙头敲进这里。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。