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Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Take advantage of skin after washing face not parching , use salt to scribble just the right amount on the nose since enclosing massage , will be clean feeling that salt melts slowly , continue massage , will be OK to use clear water scrubbing after 10 minutes or so.

洗脸后趁皮肤没有干透，用盐适量涂在鼻子上打圈圈按摩，会感觉盐慢慢融化，继续按摩，过10分钟左右即可用清水洗干净。

After some passionate Expressions of the old Acquaintance, I enquir'd, you may be sure, after my Plantation and my Partner: The old Man told me he had not been in the Brasils for about nine Years; but that he could assure me, that when he came away, my Partner was living, but the Trustees, who I had join'd with him to take Cognizance of my Part, were both dead; that however, he believ'd that I would have a very good Account of the Improvement of the Plantation; for that upon the general Belief of my being cast away, and drown'd, my Trustees had given in the Account of the Produce of my Part of the Plantation, to the Procurator Fiscal, who had appropriated it, in Case I never came to claim it; one Third to the King, and two Thirds to the Monastery of St.

老友重逢，交谈之际，言词热切。不用说，我接着就询问了我的种植园和合股人的情况。老人家告诉我，他已有九年没有去巴西了。但他可以向我保证，当他离开那里的时候，我的合股人还在人世。我曾委托他和另外两位代理人照管我的产业。尽管那两位代理人已经过世，但他相信，关于我那种植园的收益，我还是不难收到一份种植园这几十年来发展的详细报告。

After comparing the examination result with the common biological indicators, we notice that the organic substances in the samples collected from the mud volcanoes mainly come from land higher plants and the result of the redeposit after washing out the upstream's deposit, this conforms the geographic location in the mountains and the luxuriant plants in the region, hoping this research result can provide reference values for the future land developers, in order to take advantage of the land as much as possible, aw well as a perfect planning and conservation of the mud volcano.

将检测结果与常见的生物指标比对后，发现所取泥火山样品中所含的有机物质主要来自於陆生高等植物及上游沉积物经冲刷后再沉积的结果，这和泥火山的地理位置位於山区且附近植被茂盛是相符合，希望这样的研究结果可提供将来土地开发者参考，对这块土地做最好的利用，并对泥火山做完善的规划与保留。

After the posters should pay attention to the lunch time, during this period were more traffic, the best restaurant in the export of leaflets, this time we will not worry because the bowl and no time to attend our publicity posters take the focus-bombing Law paste, concentrated in a row for a space paste posters, even if such coverage was still a few left behind, brother of the club to share this experience after all, this is a good problem-solving approach.

以后贴海报注意把握应该在午饭时间，这段时间人流量比较多，最好在餐厅出口发传单，这时大家都不会因为着急抢饭而无暇顾及我们的宣传，海报采取集中轰炸式贴法，集中在一段空间贴一排海报，这样即使被覆盖了几张还是有留下来的，兄弟俱乐部分享的这一经验这也不失为解决问题的一个好办法。

After the intermediate stage in the Medieval Europe, the deed theories of Hebrew culture was not only enormously promoted the economic development , with contracting freedom inherited from the ancient Rome culture, Moreover, it had expedited the people sovereignty theories. After the combination of the neoteric commodity economy with the theories of Christianity\'s original sin, belief doctrine, and the wealth view, which gave birth to the supreme status of constitution, and the conception of equality, freedom, and so on. Christianity\'s wealth view (especially the Protestantism ethics) enormously promoted the capitalism expansion and finally forms the conception that private property are sacrosanctity. The ideology of successive authority restriction from Christianity, and the Christianity\'s exercise to partial legislative power and jurisdiction power, combined with autonomous cities based on commodity economy, and the political phenomenon that merchants exerted legislative power and jurisdiction power. Those had made the mundane ruler such as king, and so on, could only take the exertion of tax revenue power as the main symbol administrative authorities. It provided historical and psychological foundation for the birth of modern decentralization theories .

希伯来文化中的"约"和古罗马的契约自由不仅极大地促进中世纪中期以后欧洲商品经济的发展，而且催生了人民主权理念；基督教的"原罪说"、信仰主义和财富观等与近代商品经济的结合，生成了宪法至上及平等、自由等权利观念；基督教的财富观极大地促进了资本主义扩大再生产的实现并最终形成私有财产神圣不可侵犯的财产权观念；基督教会传承的权力制约思想及基督教会对部分立法权和司法权的行使，与建立在商品经济基础上的自治城市及商人行使立法权、司法权等政治现象相耦合，使得国王等世俗的统治者习惯于只行使以税收权为主要标志的行政权，为近代分权学说的产生和分权制度的建立提供了历史和心理的基础。

After the intermediate stage in the Medieval Europe, the deed theories of Hebrew culture was not only enormously promoted the economic development , with contracting freedom inherited from the ancient Rome culture, Moreover, it had expedited the people sovereignty theories. After the combination of the neoteric commodity economy with the theories of Christianity"s original sin, belief doctrine, and the wealth view, which gave birth to the supreme status of constitution, and the conception of equality, freedom, and so on. Christianity"s wealth view (especially the Protestantism ethics) enormously promoted the capitalism expansion and finally forms the conception that private property are sacrosanctity. The ideology of successive authority restriction from Christianity, and the Christianitys exercise to partial legislative power and jurisdiction power, combined with autonomous cities based on commodity economy, and the political phenomenon that merchants exerted legislative power and jurisdiction power. Those had made the mundane ruler such as king, and so on, could only take the exertion of tax revenue power as the main symbol administrative authorities. It provided historical and psychological foundation for the birth of modern decentralization theories .

希伯来文化中的"约"和古罗马的契约自由不仅极大地促进中世纪中期以后欧洲商品经济的发展，而且催生了人民主权理念；基督教的"原罪说"、信仰主义和财富观等与近代商品经济的结合，生成了宪法至上及平等、自由等权利观念；基督教的财富观极大地促进了资本主义扩大再生产的实现并最终形成私有财产神圣不可侵犯的财产权观念；基督教会传承的权力制约思想及基督教会对部分立法权和司法权的行使，与建立在商品经济基础上的自治城市及商人行使立法权、司法权等政治现象相耦合，使得国王等世俗的统治者习惯于只行使以税收权为主要标志的行政权，为近代分权学说的产生和分权制度的建立提供了历史和心理的基础。

For example, housing loans will be a day late to do the implementation of the new interest rate increase; bought the bonds, after raising interest rates to one count, savings bond cost-effective than the …… So, as a prudent investor should not be in or income after the deal or remorse incessantly, but should take precautions before the rate hike, do a good job in a variety of preparations.

例如，住房贷款将每天晚做执行新的加息；购买债券，加息后一项，储蓄债券成本效益比……因此，作为一个精明的投资者不应该或收入后的交易或悔恨不断，但应采取预防措施，加息前，做好了各种准备工作。

In the process ofnavigation of INS/Scene Matching the aerocraft will save the land information of undersurface offly orbit in the memorizer which has been scouted in advance. While the aerocraft fly thescheduled position the sensor schlep will take the real time image like as RAR image. After thenthe real time image will match the reference image which has been stored beforehand and hasgeographic relation with real time image. Thus the position or horizontal and longitudinal distanceerror of the aerocraft can been fixed on. After that the navigation and control and guidance iscomplete.

在INS／景象匹配组合导航系统的导航过程中，飞行器首先将预定飞行轨道下面事先侦察到的一系列地面图像信息存到飞行器的存储器中，然后，当携带相应传感器的飞行器飞行到预定的位置时，便获取实时图，然后将实时图和预存的已知地理信息关系的基准图进行匹配比较，这样就可以确定出飞行器的当前位置或者偏离预定位置的纵向和横向偏差，从而完成导航和制导功能。

Silk gland cell, by the Grace nutrient fluid in vitro culture, then after 150d for primary culture, passage was taken, later each approximately 15d take a passage, after that, the time of passage cut to 10 day.

经210d左右的原代培养后进行传代培养，以后每隔约15d传代一次，后传代时间缩短到10天，当传代时间稳定在7天时细胞开始趋于稳定，此时鉴定细胞系的生物学特性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。