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The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Expansive soil is a special dauby soil which will intenerate and expand after sopping, but will shrink and craze after losing water.

膨胀土是一种吸水膨胀软化、失水收缩开裂的特种粘性土。

Expand the secondhand goods that debus comes to is torn open to wear after storeroom, make continue to use after maintenance entirely.

扩展库房后拆卸下来的旧货架，全部都进行维修后继续使用。

The map hr before decannulation and after 1min ,3min , 5min.3 .the morbidity rate of laryngopharyngitis within 24 hours after operation.

观察记录各组：①术后病人清醒时的耐管效果。②拔管前、拔管即刻、拔管后1、3、5min的平均动脉压、心率。

The MAP HR before decannulation and after 1min ,3min , 5min.3 .the morbidity rate of laryngopharyngitis within 24 hours after operation.

对照组病例在吸痰拔管后即刻出现MAP升高、HR加快，并延续至拔管后3min ，与拔管前相比差异有显著性(P.05)， 5min时恢复正常。

The MAP HR before decannulation and after 1min ,3min , 5min.3 .the morbidity rate of laryngopharyngitis within 24 hours after operation. Result: All the operation were finished in 2 hours.

对照组在麻醉诱导成功后用生理盐水3ml对咽喉部、气管内表面喷雾，面罩给氧3min后插入气管导管。

The average tilt of IOL in PMMA group is 2.57±1.34° in 8 weeks after surgery, and the average decentration of IOL is 0.41±0.18mm; the average tilt of IOL in silicon group is 4.25±2.37° in 8 weeks after surgery, and the average decentration of IOL is 0.72±1.04mm.

PMMA人工水晶体组在术后八周时平均倾斜度为2.57±1.34°，平均中心移位0.41±0.18mm；Silicone组则平约倾斜度为4.25±2.37°，平均中心移位为0.72±1.04mm。

Compared with stress relaxation of treated wet wood, it was found that the plasticity of wood increased greatly after decrystallization treatment, but it obviously decreased again after drying.

对比分析非晶化湿材的应力松弛，发现非晶化处理后，木材的塑性大大增加，但是干燥后，塑性却又大大减小。

After treatment the therapeutic effect, the defervescent time, and the changes of ALT,BUN before and after treatment and adverse reactions were observed for both groups.

观察2组用药后临床疗效、退热时间、用药前后血丙氨酸氨基转移酶、尿素氮的变化及不良反应发生情况。

Biomass in the rain forest was far more than that in the plantation of 12 years,especially for the shrub layer.But the biomass of litterfall collected in the plantation was greater than that of the rain forest for the reason that Betula alnoidis defoliated in dry seasons.9 Four years observation found that soil bulk density tended to increase first, then decrease after planting trees;total soil porosity and non-capillary porosity tended to decrease and after few years they recovered to different degrees;the soil cation exchange capacity for all forest types increased;in the soil of 0-30cm depth,soil salt saturation tended to decrease;nutrient content in the soil of 0-60cm depth in the young stand tended to decline;soil effective P,effective K and N contents all tended to decrease.

山地雨林的总生物量远远大于10年生的西南桦人工林，其中灌木层的相对差别尤其巨大。94次的观测发现，人工林栽植后，土壤容重一般呈现一个先上升后下降的特点；而土壤非毛管孔隙度、土壤总孔隙度大多呈下降趋势，几年后有不同程度的恢复。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。