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It was purified by immobilized metal ion affinity chromatography under native conditions because there was a 6 histidines tag at its amino acid terminus.

在间接ELISA和免疫印迹试验中，重组的基质蛋白可与马传贫阳性血清样品发生反应，而与健康马血清无任何反应。

Coli. The protein was expressed in a soluble form and as a fusion protein. There was a 6 histidines tag at its amino acid terminus. It was purified by immobilized metal ion affinity chromatography under native conditions.

所表达的蛋白是一种可溶性的融合蛋白，其氨基端带有6个组氨酸的标签，因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化。

The HμOR overexpressed in this study, with a tag of six consecutive histidines at its carboxyl terminus, will facilitate the future studies on exploration of reconstitution and structural basis of ligand recognition of HμOR.

我们在HμOR的C-端锚定六个连续的组氨酸（6His），将有利于HμOR的分离纯化，研究HμOR的重组和配体识别的结构基础。

The cDNA of human μOR with a six consecutive histidines tag at its carboxyl terminus had been introduced in the baculovirus genome under the control of the polyhedrin promoter.

含目的基因HμOR-6His cDNA的基因片段被整合在转录多角体蛋白基因的强启动子控制下的杆状病毒基因组中。

The codons encoding a tag of six consecutive histidines was added to 3'-end of human μOR cDNA by polymerase chain reaction with primer pair 5'-AATACG ACTCACTATAG-3' and 5'-AAAGGTCGACTAATTATCATTAGTGATGGTGATGG TGATGGGGCAACGGAGCAGTTTCTGC-3'.

本研究通过PCR方法在人μ阿片受体cDNA的3'端锚定编码六个连续组氨酸的密码子，PCR产物亚克隆人质粒载体pBluescriptⅡKS+／-的Hind Ⅲ／Sal Ⅰ位点。

Objective: Construct a lentiviral expression vector containning a scavenger receptor that binds uniquely phosphatidylsedne and oxidized lipoprotein with the six histidines tag in order to investigate the function of SR-PSOX in atherosclerosis.

目的：通过构建结合磷脂酰丝氨酸和氧化脂蛋白的清道夫受体与6个组氨酸融合基因慢病毒表达载体，诱导其表达，为研究SR-PSOX在动脉粥样硬化中的作用提供技术平台，探讨该基因是否通过TNF-α而参与AS的形成过程。

The full hBD2 cDNA was inserted into the multi clone site of a eukaryotic expressive plasmid pcDNA3.1/Myc His and located closely at the upstream of two tag gene (myc and 6 Poly histidines ) so as to construct another recombinant eukaryotic expressive vector of hBD 2 gene: rpcDNA3.1/Myc Hi...

将 h BD- 2的全长 c DNA片段插入编码双重报告基因 Myc和 6个多聚组氨酸的真核表达质粒 pc DNA3.1/Myc- His ，构建出 C端带 Myc和 6× His的 rpc DNA3.1/Myc-His /h BD- 2。

I wanted to explore long-lasting love and its possible steep price tag, homophobic antipathy and denial.

我想深层次挖掘持续长久的爱情故事，基于同性的，同情的，不被人谅解的。

I want to open an account in your bank, do I need to take the identification tag?

我想在你们银行开户，我要先取号码牌吗？

I hope to open an account in your bank, do I need to take the identification tag?

我想在你们银行开户，我要先取号码牌吗？

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。