查询词典 tag-end

Ken also revealed to him that he had learnt many years after he had retired that both he and Mac apart from a dozen other CIA staff had also been unsuspectingly subject to the Kalpa Vigraha experiment before being allotted their watering team assignment. Both men shed tears following this disclosure. It was deeply disturbing now despite the loyalty with which they had served the agency.

其实，对全世界所有那些被喂过水的受试者，CIA一直都保持着极其严格的跟踪和联系（CIA就称他们为&劫号&，即'Kalpa-tag'），并秘密跟踪他们的生活状况。

A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物，自接种H5N1亚型病毒的鸡胚组织中提取RNA，反转录后采用高可信度DNA聚合酶经PCR扩增M1基因，采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达，纯化获得N末端携带多聚组氨酸标签的重组蛋白，分子量约29.8 kDa。

XIelwou're awesome! And by the way, TAG, you're it. As amazing and awesome as you already are, you can be even more so. Beautiful young people are the whimsey of nature, but beautiful old people are true works of art. But you don't become "beautiful" just by virtue of the aging process.

你 卓越的！没错就你你已经了不起的卓越的你还可以更卓越更了不起美丽的年轻人大自然的奇想而美丽的老人却艺术的杰作但你不会因为年龄的渐长就自然而然地变得&美丽&

The supervisor is required to sign the reject tag if he/she concurs with the reject status.

对所有的检验员和主管进行新的作业指导书的培训。

And, last month, the Government announced plans to tag cigarette packs to allow customs officials to determine whether duty has been paid.

和，上个月，政府宣布，计划在卷烟烟盒上标记，让海关官员，以确定是否有责任已支付。

And, last month, the Government announced plans to tag cigarette packs to allow customs officials to determine whether duty has been paid.

和，上个月，政府宣布，计划在卷菸烟盒上标记，让海关官员，以确定是否有责任已支付。

Use filename as a localisation tag for information.

使用文件名作为地方化标记对于信息。

The results showed that the optimum concentration of five important components i.e. Tag DNA polymerase, Mg2+, primer, template DNA and dNTPs in 25μL RAPD reaction system were 1.0u, 2.5mM, 20ng, 40ng and 0.2mM, respectively. The RAPD fingerprinting of five Japanese plum cultivars were established by using optimum reaction system with ten random primers.

结果表明：1、改良陈大明法是提取中国李叶片基因组DNA的适宜方法。2、以5′-GACCGCTTGT-3′为随机引物，建立了优化的中国李RAPD反应分析体系：25μL反应体系中，Taq酶、Mg~(2+)、引物、模板DNA和dNTPs 5种主要成分的适宜浓度或用量分别是：1.0u、2.5mmol·L~(-1)、20ng、40ng和0.2mmol·L~(-1)。

The ISSR fingerprinting of 18 Japanese plum cultivars and 12 plum species or variety were established by using optimum system with primer 5"-9T-3"and 5"-9C-3", respectively. The identification rate of plum cultivars and species or variety was 100%. The effects of five important components on reaction of SSR were studied with Prunus salicina cv. Meilili. The results showed that the optimum concentration of five important components i.e. Tag DNA polymerase, Mg +, single primer, template DNA and dNTPs in 25 μL reaction system of SSR were 1.5u, 2.0mM, 0.8 μ M, 30~40ng and 0.16~0.24mM, respectively. The dendrograms of these cultivars was contructed according the Jaccard coefficient of similarity.

利用该优化体系，以5′-_9T-3′为引物，构建了中国李18个品种的ISSR指纹图谱，该引物可将这些品种完全区别开来；以5′-_9C-3′为引物，构建了李属6类种质资源的ISSR指纹图谱，该引物区分率为100％。4、中国李品种适宜的SSR反应分析体系是：25μL SSR反应体系中，Taq DNA聚合酶、Mg~(2+)、每个引物、模板DNA和dNTPs等5种成分的适宜浓度分别是：1.5u、2.0mmol·L~(-1)、0.8μmol·L~(-1)、30～40ng和0.16～0.24mmol·L~(-1)。5、采用RAPD、ISSR和SSR等三种分子标记技术对中国李品种资源54份和2个欧洲李品种进行了遗传多样性分析，并以品种间的遗传相似系数构建了Jaccard聚类树状图。

Such rarified company comes with a big price tag.

由于粥少人多，免费获得邀请是少之又少。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新