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Methods SMMC-7721 cell apoptosis was induced by HCPT and the mitochondria were isolated with a mitochondria isolation kit. Mitochondrial proteins labeled with a cleavable isotope-coded affinity tag were identified and quantified using two-dimensional liquid chromatography/tandem mass spectrometry.

用羟基喜树碱诱导肝癌细胞SMMC-7721凋亡，提取线粒体并鉴定其纯度；用可裂解的同位素标记的亲和标签技术标记线粒体蛋白质，利用二维液相色谱-串联质谱分析鉴定蛋白质。

Firstly, MiCy, Citrine, mKO were inser into expression vectors of pRSETB, and then MiCy, Citrine,mKO were expressed in E.coli and purified by using Ni-NTA collum chromatography.

首先，构建了含有六个组氨酸标签的三种荧光蛋白MiCy、Citrine和mKO，而后在大肠杆菌中表达了MiCy、mKO和Citrine三种蛋白，并用金属鳌合亲和层析进一步纯化，纯化产物使用特异性酶去除His-tag。

Results The tag was used in cosmically practice satisfactorily.

结果：在大规模演练中使用，效果满意。

The paper is based on the studerate cump managemf Basn system and the basic theory and method about system develop,The whole paper can be divided into six chapters iem, Tag and so on.

摘要本论文以西电基础教学实验中心学生上机管理系统为背景，论述了管理信息系统的概念，结构及系统开发的基本原理和方法。

In this thesis, we did modifications of alkyl groups upon benzyl fluorophosphonate. In order to study the influence of alkyl length and steric effect on activity and selectivity of probes, we synthesized benzyl fluorophosphonate recognition heads with modifications including ethyl, butyl, octyl and cyclohexyl. By way of connecting different kinds of tag like azido group, biotin and rhodamine with recognition heads separately through a higher hydrophilic ethylene glycol derived linker, a series of benzyl fluorophosphonate probes were made up. And they will apply to various biological testing strategies.

在本论文中，我们以苯甲基氟化磷酸酯为基础，於其上进行一系列烷基之修饰，为了探讨烷基长度与空间立体性质对於探针活性及选择性的影响，我们合成了包含乙烷基、丁烷基、辛烷基以及环己烷基修饰的苯甲基氟化磷酸酯辨识端，并藉由具较高亲水性的聚乙二醇衍生连接桥结合三种不同的发报端，分别有叠氮基团、生物素及萤光基团来构成不同的化学探针组合，这一系列苯甲基氟化磷酸酯类化学探针将可被应用於不同的生物测试策略。

If tag at the back of 28 guests,be:"Foot 3 ", 300, small static, other place, child, like filar socks.

如28号客人后面标注为：&脚三&，300次，小静，外地，小孩，喜欢丝袜。

At the beginning of April 2008, when Yangzhou netizen Xiaoli is playing computer game, a window jumps out suddenly on screen, see only above tag say to click download after this program, can increase game traversal speed, see this, xiaoli is hesitant without filar a person of extraordinary powers, pressed mouse.

2008年4月初，扬州网民小李在玩电脑游戏时，屏幕上突然跳出一个窗口，只见上面标注称点击下载该程序后，可以提高游戏运行速度，见此，小李没有丝豪犹豫，便按下了鼠标。

She and Dark Magician are a formable tag-team, able to combine their powers for stronger attacks.

她和黑魔术师，是一个成型的标签-团队，能够结合自己的效果为强大的攻击。

The content includes six parts: Part one is a summary of ASP,this part has done the detailed introduction to the correlate knowledge of ASP;Part two is a summary of datebase,this part introduces how to interview datebase mostly; Part three is the analyse in demand,this part makes frondose analyse about function,performance and environmental demand for the system;Part four is a collectivity design,this part gives holistic design plan to every modules;Part five is a particular design,this part gives detailed introduction to each module's realization and design;Part six is a tag.

内容包括6个部分：第1部分是ASP概述，该部分对ASP技术的相关知识做了详细的介绍；第2部分是数据库概述，该部分主要是介绍ASP对数据库的访问；第3部分是需求分析，该部分对系统的功能，性能和环境做了具体的分析；第4部分是总体设计，该部分对系统的各个模块给出了整体的设计方案；第5部分是详细设计，该部分分别对各个功能模块的实现与设计做的详细的介绍；第6部分是结束语。

The content includes six parts: Part one is a summary of ASP,this part has done the detailed introduction to the correlate knowledge of ASP;Part two is a summary of datebase,this part introduces how to interview datebase mostly; Part three is the analyse in demand,this part makes frondose analyse about function,performance and environmental demand for the system;Part four is a collectivity design,this part gives holistic design plan to every modules;Part five is a particular design,this part gives detailed introduction to each module's realization and design;Part six is a tag.

内容包括6个部分：第一部分是ASP概述，该部分对ASP技术的相关知识做了详细的介绍；第二部分是数据库概述，该部分主要是介绍ASP对数据库的访问；第三部分是需求分析，该部分对系统的功能，性能和环境做了具体的分析；第四部分是总体设计，该部分对系统的各个模块给出了整体的设计方案；第五部分是详细设计，该部分分别对各个功能模块的实现与设计做的详细的介绍；第六部分是结束语。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新