查询词典 tag up

Shandong sinder technology limited company,qingdao 266061,shandong,chinaabstract:to provide the eukaryotic expression vector carrying 6x histidine-tag and human growth hormone in expremental animal and cell culture,according to the published nucleotide sequence of the lactofericin b and human growth hormone,synthesis sequence was ligated with pmt-18 vector.

以牛乳素为目的基因，构建含有组氨酸标签、人生长激素信号肽的真核表达载体，为利用牛乳素在活体动物及培养细胞中的表达提供基因材料。

In a real-world application, instead of running the Save to Tag code from a command button, you would probably want to run it from some appropriate event, such as moving to another section of a form, perhaps when entering a subform.

注意：在实际的应用中，你可能想在某个合适的事件中运行代码，而不是用 Save 来运行代码。比如，在转到窗体的其它区段时，也可能是在进入子窗体时。

The first template parameter gives the subrule an identification tag.

第一个模板参数给了子规则一个身份标签。

You have to tag the person who tagged you.

你必须回tag那个tag你的人。

The company is committed to the production of food / drink / candy / Household Cleaning Products / Chemicals, such as packaging materials, the main products include jellies and beverages tectorial membrane sealing / self-reliance with Tsui BAG / BAG beverages / frozen food packaging bag / vacuum bag / pull chain bag / sack / candy and small food (extruded, etc.) packaging bags / film, various uses, such as tag and bag shaped.

本公司主要致力于生产食品/飲料/糖果/日用洗涤用品/化工等的包装材料，主要产品包括果冻和飲料封口盖膜/带咀自立袋/飲料袋/速冻食品包装袋/真空袋/拉鏈袋/米袋/糖果及小食品包装袋/膜，各类用途的标签和異型袋子等。

This is used in Tiberian Sun to give two Super Weapons to the Missile Silo, however one of those Super Weapon has this tag set for the Waste Facility which means you lose the weapon if you lose the Waste Facility even though the Missile Silo actually has the weapon.

此句在Tiberian Sun中用于给导弹井添加2个超武。不管如何，其中的一个超武关联到了废物工厂，意味着一旦你丧失废品厂，你的导弹井就无法发射。

Tortula ruralis is the most studied desiccation-tolerant species in established expressed sequence tag databases and will be an important experimental model in investigating vegetative desiccation tolerance.

山墙藓是耐旱研究较多的一个种，已建立了表达序列文库，将会成为耐旱研究的重要模式植物。

CHINA BENEFICIARY: GUANGZHOU SHARMICO TRADING CO LTD ACCOUNT NUMBER: OSA 11007495403401 SHENZHEN DEVELOPMENT BANK CO LTD SWIFT BIC: SZDBCNBS IS NOMINATED AS TRANSFERRER BANK THE ARTICLES 41 OF UCP500 IS NOT AVAILABLE PARTIAL SHIPMENTS ALLOWED ONLY PER FULL CONTAINERS EACH ITEM MUST HAVE HANG TAG OR LABEL WITH BARCODE NUMBER, ITEM TYPE, IMPORTATO DA GRANDE

中国受益人：广州SHARMICO 贸易的有限公司CO 帐号： OSA 11007495403401 深圳开发银行有限公司CO 快速BIC： SZDBCNBS 被提名当文章41 UCP500 不是可利用的部份发货允许只每充分的容器各个项目必须有吊标记或标签以BARCODE 数字的发件人银行，项目类型， IMPORTATO DA GRANDE

Methods: the contents of amino acids are determined by hplc; tryp is determined by pico.tag; vita, vitd, vite, vitb1, vitb2, vitb6, vitb12, vpp, folic acid of formica rufa l. are determined by hplc. results: the amino acids of formica rufa l.

采用高压液相色谱仪测定蛋白质中17种氨基酸成分与含量，使用pico.tag柱前衍生法测定色氨酸成分与含量；使用高压液相色谱法测定维生素a、e、d、b1、b2、b6、b12、烟酸、叶酸等含量。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。