查询词典 tag question

Tortula ruralis is the most studied desiccation-tolerant species in established expressed sequence tag databases and will be an important experimental model in investigating vegetative desiccation tolerance.

山墙藓是耐旱研究较多的一个种，已建立了表达序列文库，将会成为耐旱研究的重要模式植物。

CHINA BENEFICIARY: GUANGZHOU SHARMICO TRADING CO LTD ACCOUNT NUMBER: OSA 11007495403401 SHENZHEN DEVELOPMENT BANK CO LTD SWIFT BIC: SZDBCNBS IS NOMINATED AS TRANSFERRER BANK THE ARTICLES 41 OF UCP500 IS NOT AVAILABLE PARTIAL SHIPMENTS ALLOWED ONLY PER FULL CONTAINERS EACH ITEM MUST HAVE HANG TAG OR LABEL WITH BARCODE NUMBER, ITEM TYPE, IMPORTATO DA GRANDE

中国受益人：广州SHARMICO 贸易的有限公司CO 帐号： OSA 11007495403401 深圳开发银行有限公司CO 快速BIC： SZDBCNBS 被提名当文章41 UCP500 不是可利用的部份发货允许只每充分的容器各个项目必须有吊标记或标签以BARCODE 数字的发件人银行，项目类型， IMPORTATO DA GRANDE

Methods: the contents of amino acids are determined by hplc; tryp is determined by pico.tag; vita, vitd, vite, vitb1, vitb2, vitb6, vitb12, vpp, folic acid of formica rufa l. are determined by hplc. results: the amino acids of formica rufa l.

采用高压液相色谱仪测定蛋白质中17种氨基酸成分与含量，使用pico.tag柱前衍生法测定色氨酸成分与含量；使用高压液相色谱法测定维生素a、e、d、b1、b2、b6、b12、烟酸、叶酸等含量。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Methods: Two tag SNPs (rs2439322 and rs16879809) of NRG-1 in 254 schizophrenia trios were genotyped by using PCR-RFLP, and LD, TDT and HHRR were analyzed by using EMLD, UNPHASED and TRANSMIT softwares.

目的 ：探索位于 8 号染色体短臂（ 8p ）的神经胶质细胞生子因子 1 （ NRG-1 ）基因与精神分裂症易感性之间的关系；探索同样位于 8p 的 NRG-1 基因与 ASAH1 基因之间的相互作用。

Methods : Two tag SNPs (rs2439322 and rs16879809) of NRG-1 in 254 schizophrenia trios were genotyped by using PCR-RFLP, and LD, TDT and HHRR were analyzed by using EMLD, UNPHASED and TRANSMIT softwares.

应用 PCR-RFLP 检测 254 个精神分裂症核心家系 NRG-1 基因上的 2 个标签 SNPs （ rs2439322 和 rs16879809 ），进行 LD 、 TDT 、 HHRR 及单体型 TDT 分析。

A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物，自接种H5N1亚型病毒的鸡胚组织中提取RNA，反转录后采用高可信度DNA聚合酶经PCR扩增M1基因，采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达，纯化获得N末端携带多聚组氨酸标签的重组蛋白，分子量约29.8 kDa。

XIelwou're awesome! And by the way, TAG, you're it. As amazing and awesome as you already are, you can be even more so. Beautiful young people are the whimsey of nature, but beautiful old people are true works of art. But you don't become "beautiful" just by virtue of the aging process.

你 卓越的！没错就你你已经了不起的卓越的你还可以更卓越更了不起美丽的年轻人大自然的奇想而美丽的老人却艺术的杰作但你不会因为年龄的渐长就自然而然地变得&美丽&

The supervisor is required to sign the reject tag if he/she concurs with the reject status.

对所有的检验员和主管进行新的作业指导书的培训。

And, last month, the Government announced plans to tag cigarette packs to allow customs officials to determine whether duty has been paid.

和，上个月，政府宣布，计划在卷烟烟盒上标记，让海关官员，以确定是否有责任已支付。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。