查询词典 tag end

So one time I got your creel case open the night before you went on one a your little trips -- price tag still on it after five years -- and I tied a note on the end of the line.

于是，在你又要出去钓鱼的前一天晚上，我打开了你的鱼篮子。五年前的价格签还在那儿挂着呢。

He tag ged a request for more money to the end of his speech.

他在讲话结束前附带提出要求更多的钱。

If you want an image on your web page use the html tag below and place a at the end.

如果你想对你的形象网页使用的HTML标签以下、在结束了。

If you make a statement and add a question on the end of it, you're making a tag question.

反意疑问句就是前一部分是一个陈述句，后面再跟一个问题。

The hand painted ceilings, the horizonless pool, the master suite, the 10,000 square feet, the 14-million-dollar price 4 tag on this home is no where near the top end.

在有些城镇，你在特定时段里是不能发出噪音的——从十月到四月你不能发出任

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

End the practice of awarding business on the basis of price tag.

停止依价格为基础的决标方式。

The codons encoding a tag of six consecutive histidines was added to 3'-end of human μOR cDNA by polymerase chain reaction with primer pair 5'-AATACG ACTCACTATAG-3' and 5'-AAAGGTCGACTAATTATCATTAGTGATGGTGATGG TGATGGGGCAACGGAGCAGTTTCTGC-3'.

本研究通过PCR方法在人μ阿片受体cDNA的3'端锚定编码六个连续组氨酸的密码子，PCR产物亚克隆人质粒载体pBluescriptⅡKS+／-的Hind Ⅲ／Sal Ⅰ位点。

Runs at the end of the admin panel inside the body tag.

在标签内，管理面板的结尾，运行。

It then uses RegisterStartupScript to emit the script include tag at the end of the form.

然后，它使用RegisterStartupScript排放的脚本包括在窗体的结束标记。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。