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Objective: The first aim of this study was to investigate bcr-abl associated T cell receptorVαand Vβgene repertoire by analyzing the restricted usage and clonal expansion of TCRVαand Vβsubfamily T cells from the normal individual peripheral blood induced bybcr-abl peptideso The second aim was to construct the idiotype TCR Vα-PIRES-TCR Vβeukaryotic vector and investigate its expression in vitro. The data will be expected toprovided the methods in development of the leukemia antigen specific cytotoxic Tlymphocyte modified by the specific TCR.
目的:通过分析bcr-abl多肽诱导正常人T细胞克隆性增殖和TCR Vα和TCR Vβ基因选用情况,获得识别bcr-abl多肽的特异性TCR基因,并构建TCR Vα和TCR Vβ双基因表达载体及分析其体外转染细胞和表达特点,从而为研制TCR转基因获得白血病抗原特异T细胞的研究提供实验依据。
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The results showed that biomass of Eucalyptus exserta stands were increscent with the increasing of stand age.It was 2.77 t/hm2 in the stand of 0.7 year and was 70.932 t/hm2 in the stand of 7.7 years.
结果表明,窿缘桉林分的生物量是随着林分年龄的增长而增加,即由造林初期(0.7 a生)的2.77 t/hm2增加到主伐期(7.7 a生)的70.932 t/hm2。
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Extant jawless vertebrates, represented by lampreys and hagfishes, have innate immune receptors with variable domains structurally resembling T/B-cell receptors. However, they lack cardinal elements of adaptive immunity shared by all jawed vertebrates: T/B-cell receptors and major histocompatibility complex molecules.
在以七鳃鳗和盲鳗为代表的无颌类脊椎动物中,虽然发现了与有颌类脊椎动物T细胞受体(T-cell receptors, TLRs)、B细胞受体(B-cell receptors, BCRs)可变区具有相似结构的先天性免疫受体,却从未发现有颌类脊椎动物适应性免疫系统的核心组分:TCRs、BCRs、组织相容性复合体(Major histocompatibility complex, MHC)。
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It is clear that A restricted on ker T is : identity, which is compact, it must hold that ker T is finite-dimensional.
虽然ker T的有限维可以直接用反证法得出。利用ker T的有限维性质也应该是最简单的证法了吧多谢解答!
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Cell line analysis showed that the antibody notably reacted to three out of 4 cell lines (Raji, SMS-SB, Nalm-6 and Nall-1) with the positive rates of 98.78%, 98.61%, 94.93% respectively and weakly to one of them with 5.68% in B lineage cell lines and monoblastic cell line (U937, 67.78%) while it was only weakly positive or negative for other myeloid leukemia cell lines including Meg01 (33.40%), HL-60 (29.70%),K562 (28.19%), KG1a (16.23 %) and HEL92.1.7 (8.02%). Among 4 T lineage leukemia,5 neuroblastoma and 1 colon cancer cell lines tested, only Molt-3 was found weakly positive (31.40%) for 2B8a, while the remaining 3 T cell lines (Molt4, JM and CCRF-CEM), 5 neuroblastoma cell lines (LA-N1, KCNR, BE, SK-N-SH, SK-N-AS) and the colon cancer cell line (HR8348) tested were negative.
结果表明: 2B8a抗原在外周血B细胞上表达(3/3例,平均阳性细胞数为26.29 %),而在T淋巴细胞和NK细胞上不表达(0/3例);在粒细胞和单核细胞上阳性表达均为2/3例,平均阳性细胞数分别是23.72 %和59.84 %;在DC细胞、红细胞和血小板上均不表达(0/3例)。2B8a抗原在骨髓CD34+细胞上的阳性表达是3/3例,平均阳性细胞数39.33 %,而在G-CSF动员的外周血CD34 细胞上的阳性表达仅1/3例,平均阳性细胞数为1.25 %。2B8a抗原在B系细胞系Raji、SMS-SB、Nalm-6和Nall-1上的平均阳性细胞数分别为98.78 %、98.61 %、94.93 %和5.68 %;在T系细胞系Molt-3上的平均阳性细胞数为31.40 %,而在Molt-4、JM和CCRF-CEM 细胞上不表达;在髓系细胞系U937、Meg-01、HL-60、K562、KG1a和HEL92.1.7上的平均阳性细胞数分别为67.78 %、33.40 %、29.70 %、28.19 %、16.23 %和8.02 %;在神经母细胞瘤细胞系SK-N-SH、KCNR、BE、LAN-1和SK-N-AS细胞以及结肠癌细胞系HR8348细胞上均不表达,而在羊膜细胞系FL细胞上呈一定的阳性表达,平均阳性细胞数为45.03%。
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They all contain 13 protein coding gene,2 rRNA gene,22 tRNA gene and 1 D-Loop.The base composition for the four nucleotides is A-32.0%,C-27.6%,G-14.7%,T-25.8%,And it is A-32.5%,C-26.9%,G-14.1%,T-26.5%for Yellow-throated Marten.But there are some definite differences in base composition,the using of Initiation codon and Stop codon,and the mode of repeat sequences in control region.The codon usages of Manes have bias,and the ttiird locations of codon of protein-coding genes have the higher frequency in using A and C.There may be some relativity with the content of A and C in D-loop,namely,it has relation with the mode of repeat sequences.The complete mitochondrial genome of the Sable and Yellow-throated Marten were submitted to GenBank,and the accession number are FJ429093 and FJ719367 respectively.3、The complete mitochondrial genome of 6 other species of Mustelidae from GenBank and some sequences of D-loop from the 6 species were aligned.
分析紫貂大兴安岭亚种、长白山亚种、阿尔泰亚种和北欧亚种间的基因流及进化历史得知:大兴安岭种群与新疆种群和长白山种群间的基因流水平最高(Nm=0.1260和0.1427),新疆与长白山种群间最低Nm=0.0053紫貂种群在进化过程中可能发生过种群膨胀,经历过种群增长过程。2、对紫貂和黄喉貂的线粒体全基因组结构进行分析发现:全长分别为16 523bp和16549bp,均包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码序列区(D-Loop区,紫貂全序列中碱基组成为A-32.0%,C-27.6%,G-14.7%,T-25.8%,黄喉貂为A-32.5%,C-26.9%,G-14.1%,T-26.5%;基因排列顺序与日本貂和貂熊的一致,但碱基组成、起始密码子和终止密码子的使用及控制区中串联重复序列模式等均存在一定差异。
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Western Blot indicated that AIF was selectively released before cytochrome C from the intermembrane space of mitochondria to cytosol, and that release needed Bax relocation to mitochondria. Results above suggest that caspase-independent cell death maybe occurred earlier than caspase-dependent cell death in staurosporine induced T lymphocytes apoptosis. Caspase-independent cell death is an alternative pathway of cell death which could guarantee the safe and noninflammatory removal of corpses when caspase was mutented or blocked in the cell, it plays an important role in maintaining the number of T lymphocytes and ensuring the proper function of immune system.
细胞caspase非依赖死亡方式可能是Caspase依赖性死亡方式的替代途径,它可以保证在Caspase发生突变或通路受阻时,T细胞仍然能够正常死亡,这对于维持T细胞的数量和保证免疫系统正常功能的发挥具有十分重要的意义。
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In addition, it also leads to the increase of T〓 and FT〓 content, the decrease of T〓 and FT〓 content, the increase of TSH content in the plasma, which seriously influences the whole body's growth and metabolism of nutrimental materials and is one of the mechnism of nutritional anemia that is caused by lack of matter of making blood.
首次揭示雏鸡硒缺乏不但对甲状腺造成损伤,而且还特异性地降低肝脏、肾脏等组织中ID-1活性,使血浆3-5-3'-5'-四碘甲状腺原氨酸、游离T〓含量升高,3-5-3'-三碘甲状腺原氨酸、游离T〓含量下降,促甲状腺激素含量升高,严重影响整个机体的生长发育及营养物质代谢,并是导致造血物质缺乏所致的营养性贫血的机理之一。
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Methods Olfactory test was performed by odor threshold and identification test (T&T olfactometry).
方法选择上呼吸道感染后嗅觉减退或丧失患者10例,用T&T嗅觉测试法测试患者的嗅觉功能。
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Results:There were no abdominal pain and fever,jaundice faded away gradually postoperation.Biliary drainage was 300ml-1000ml/d more or less.Colour of bile changed into normal by degrees.Intra peritoneal drainage was open and the colour was light bloody.Amount of drainage dropped off day after day.Whole blood cell test, serum glutamic pyruvic transaminase,serum glutamic oxalacetic transarninase,total serum protein,serum albumin and serum globulin returned to normal in first week postoperation.Serum bilirubin,serum glutamyltranspetidase and serum alkaline phosphatase declined gradually in first week postoperation.T-tube was clipped at twelfth day postoperation.T-tube Cholangiography was clear at 24~ day postoperation.
结果:术后无上腹部绞痛、高热,黄疸逐渐减退,每日引流胆汁300ml-1000ml不等,由淡黄色混浊、内有胆泥过渡到金黄色清亮的正常胆汁,腹腔引流通畅,引流液为淡红色,量从最初的200ml迅速过渡到10ml,血常规、血清谷丙转氨酶、谷草转氨酶、总蛋白、白蛋白、球蛋白等1周内均恢复正常,血清总胆红素、直接胆红素、间接胆红素、谷氨酰转肽酶、碱性磷酸酶逐渐下降,术后12d夹闭T管,术后24d T管造影提示左、右肝管及胆总管下端通畅,无狭窄。
- 相关中文对照歌词
- I Can't Change The Way You Don't Feel
- T.N.T.
- It Ain't A Party - If You Can't Come 'Round
- Don't Mind If I Don't
- I Don't Want To Be On T.V.
- If It Ain't Dixie (It Won't Do)
- It Isn't, It Wasn't, It Ain't Never Gonna Be
- V.T.T.L.O.T.F.D.G.F.
- K.O.T.T.O.N.M.O.U.T.H. Song
- H.T.H.D.T.G.T. (How The Hell Did They Get There)
- 推荐网络例句
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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.
索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。
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The relationship between communicative competence and grammar teaching should be that of the ends and the means.
交际能力和语法的关系应该是目标与途径的关系。
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This is not paper type of business,it's people business,with such huge money involved.
这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。