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A pair of primers for amplification of σC gene was designed and synthesized according to the published Muscovy duck reovirus S4 gene sequence. The specific RT-PCR product was success-fully obtained and cloned from the Muscovy duck reovirus ZJ99 strain.

参考已发表的番鸭呼肠孤病毒的S4序列设计了1对扩增σC蛋白基因的引物，对mDRV ZJ99株的RNA抽提物进行RT-PCR扩增，获得了特异性的扩增片段。

In order to develop a safe and effective immunoadjuvant to enhance the immunity and resistance of animals against infection, a novel CpG Oligodeoxynucleotides containing 11 CpG motifs was synthesized and inserted into the VR1012 plasmid, designated as VR1C. Then the recombinant VR1C was entrapped with Chitosan nanoparticles prepared by the method of ionic cross linkage, and employed to inject muscularly 3-weeks old Kunming mice; the blank VR1012 packed with CNP and saline were used to inoculate mice as the control groups. 28 days after inoculation, all mice were orally fed with 0.4ml 2x108CFU/ per mouse virulent hemorrhagic enteritis E. coli to challenge the resistance against infection.

为研制安全高效免疫调节剂增强动物免疫抗病能力，本实验设计合成含11个C pG基序的寡聚核苷酸，重组构建含CpG的VR1012质粒(VR1C)；制备壳聚糖纳米颗粒包裹重组质粒(CNP-VR1C)，肌注接种3周龄昆明小白鼠，设壳聚糖包裹空质粒和生理盐水对照组；接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力，Sandwich ELISA测定血清免疫球蛋白和白细胞介素含量。

LIN Hailin,CUI Yingde,DU Lin,CHEN JianpingInstitute of Applied Chemistry,Zhongkai University of Agriculture and TechnologySuper water absorbent resins P,SA/NaAA,SA/NaAA/PVA and SA/NaAA/AM were synthesized from sodium alginate,acrylic acid,polyvinyl alcoholand acrylamide,with N,N–methylenebisacrylamide as crosslinking agent,(NH4)2S2O8 - NaHSO3 as initiator,by means of aqueous solution polymerization.

以醚化海藻酸钠、聚乙烯醇、丙烯酰胺、丙烯酸为单体，N， N–亚甲基双丙烯酰胺为交联剂，过硫酸铵-亚硫酸氢钠为引发剂，采用水溶液法制备聚丙烯酸钠p吸水树脂和海藻酸钠/丙烯酸钠、海藻酸钠/丙烯酸钠/聚乙烯醇（SA/NaAA/PVA）及海藻酸钠/丙烯酸钠/丙烯酰胺吸水性复合树脂。

Three kinds of naphthalene containing poly-amino-compounds with different chain length have been synthesized in this work.

合成了三种含萘但链长不同的多胺类化合物。

A series of multi-pedal amino-compound with naphthol groups have been synthesized in this work. The photo-physical behaviors of these compounds in different solvents and different pH conditions have also been studied in details.

本工作合成了一系列带萘酚基的多足胺类化合物，对它们在不同溶剂及不同pH条件下的光物理行为进行了详细研究。

Naphthyl aceate was synthesized with acetic anhydride and naphthol using aminosulfonic acid as catalyst.

以氨基磺酸为催化剂，用乙酸酐和β-萘酚合成了乙酸β萘酯。

A series of 3-naphthyl-2--1,3-thiazolidin-4-ones were synthesized by the three-component one-pot cyclocondensation of naphthylamine, aromatic aldehyde and 2-mercaptoacetic acid using a microwave-assisted method in the presence of DMAP/DCC.

利用微波促进的多组分一锅法，在封管条件下，以萘胺、芳醛和巯基乙酸为原料，4-二甲氨基吡啶/N，N'-二环已基碳二亚胺为催化剂，合成了系列新的噻唑烷酮衍生物，该反应具有时间短、操作简便等优点。

METHODS: Three different MBP peptides(MBP6886, 8799, and the nonencephalitogenic peptide 110128) were synthesized and administrated nasally to Lewis rat on day11, 10, 9, 8 and 7 prior to immunization with the guinea pig MBP CFA, which was used to induce EAE.

合成3条不同的碱性髓鞘蛋白多肽（MBP6886、 8799和非致脑炎性肽段110128），在用豚鼠MBP加弗氏完全佐剂免疫Lewis大鼠前的11、 10、 9、 8和7 d，经鼻黏膜分别给予MBP多肽，观察其对EAE的保护作用。

METHODS: Three different MBP peptides(MBP6886, 8799, and the nonencephalitogenic peptide 110128) were synthesized and administrated nasally to Lewis rat on day11, 10, 9, 8 and 7 prior to immunization with the guinea pig MBP + CFA, which was used to induce EAE. The protective effect on Lewis rat from EAE by the MBP peptides was evaluated.

合成3条不同的碱性髓鞘蛋白多肽（MBP6886、 8799和非致脑炎性肽段110128），在用豚鼠MBP加弗氏完全佐剂免疫Lewis大鼠前的11、 10、 9、 8和7 d，经鼻黏膜分别给予MBP多肽，观察其对EAE的保护作用。

METHODS: Three different MBP peptides(MBP68 86, 87 99, and the non encephalitogenic peptide 110 128) were synthesized and administrated nasally to Lewis rat on day 11, 10, 9, 8 and 7 prior to immunization with the guinea pig MBP + CFA, which was used to induce EAE.

实验性自身免疫性脑脊髓炎（experimental autoimmune encephalomyelitis， EAE）是一种可致中枢神经系统瘫痪的自身免疫性疾病，为人类多发性硬化（multiple sclerosis， MS）的实验动物模型。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失