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Methods The IPSE gene of S. man soni was synthesized by overlapping PCR, and confirmed by DNA sequencing. The recombinant plasmid IPSE-pET32a was constructed by inserting the gene of IPSE into expression vector pET32a at the downstream of thioredoxin coding sequence.

应用重叠PCR方法合成曼氏血吸虫IPSE基因，将其定向克隆至表达载体pET32a的硫氧还蛋白序列下游，构建重组表达质粒IPSE/pET32a。

The results indicate that CdS nanorods synthesized by soft template method are below 100 nm in diameter and above 3 nm in length, and low irradiative dosage and concentration of reactants can accelerate the formation of CdS nanorods with higher length-to-diameter factor about 30∶1 range.

研究表明，该方法在Cd2+浓度为0.015 mol·L-1、辐照剂量为15～20 kGy时，可获得直径在100 nm以下，长度在3 μm以上的CdS纳米棒；并且较低的辐照剂量和反应物浓度有利于获得较大长径比的CdS纳米棒。

Starting with the readily available isatin, two designated key cyclization precurors 25 and 26 were synthesized respectively by 5 steps and 6 steps reactions ,with Wittig reaction and Corey-Chaykorsky reaction as key steps.

以商品可得的吲哚醌为起始原料，分别经过5步和6步以Wittig烯烃化，Corey-Chaykorsky反应为关键步骤的合成路线，得到2个用于的环合反应的前体亚胺25和26。

Odoquinazolin-4(3H)-one,7-methyl-4(3H)-quinazolinone,7-bromoquinazolin-4(3H)-one were synthesized by using anthranilic acid、2-amino-5-nitrobenzoic acid、2-amino-4-nitrobenzoic acid,6-nitro--4(3H)-quinazoline-one、o-amino-terephthalic acid, 2-amino-4-hydroxy benzoic acid、2-amino-5-bromo-benzoic acid、2-amino-5-iodine acid,1、4-butynediol、L-glutamine、isatin anhydride、formamide as starting materials and utilizing microwave-assisted synthetic approach.

本文利用微波辅助合成的方法，以邻氨基苯甲酸、2-氨基-5-硝基苯甲酸、2-氨基-4-硝基苯甲酸、邻氨基对苯二甲酸、2-氨基-4-羟基苯甲酸、2-氨基-5-溴苯甲酸、2-氨基-5-碘苯甲酸、5-甲基-2-氨基苯甲酸等为原料与甲酰胺反应，以及以1，4-丁炔二醇、L-谷氨酰胺与靛红酸酐反应，共合成了13个喹唑啉酮衍生。。。

Antioxidant 2,2′-methylene-bis(4-ethyl-6-tert-butylphenol) is synthesized by condensation of formalin and 4-ethyl-2-tert-butylphenol which is prepared by alkylation of p-ethylphenol with isobutylene in the presence of phosphoric acid or ion-exchange resin as catalysts.

研究了以对乙基苯酚、叔丁醇为原料，在酸性条件下，进行烷基化反应，合成2 叔丁基 4 乙基苯酚，再进一步缩合成抗氧剂425的工艺条件。

Complexes of Gd with alanine, Sm with isoleucine and Yb, Zn with leucine were synthesized and their crystal structures were determined.

合成了Gd与丙氨酸、Sm与异亮氨以及Yb、Zn与亮氨酸配合物单晶，并测定其晶体结构。

Studies on the characterization of molecular sieves showed that Co and Mn incorporated into the framework by isomorphous substitution of Al and both of them had the same valence (+2) in the as-synthesized and calcined samples.

它们在分子筛焙烧去除模板剂过程中价态不发生变化，均为+2价。

Simultaneously the model of bipolar junction transistor and CMOS transistor and the basic circuit units such as current mirrors are briefly introduced; with the topology and model circuit ,the principle and feature of the CC are analyzed. The applications of the CC in the fields of the active network and signal process are synthesized.

首先介绍了模拟集成电路在当代社会中的重要性和集成电路研究的必要性，并简要地介绍了双极型晶体管和CMOS晶体管的模型结构以及几种电流镜电路的原理及性能；从电流传送器的拓扑结构入手，在给出其模型电路的基础上，介绍了电流传送器的工作原理。

In this paper, a cyclic ketene acetal of 2-methylene-1,3-dioxepane was prepared. And then biodegradable SAP with C-O-C in the backbone was synthesized by Ring-Opening copolymerization of MDO with acrylic acid.

目前市场上使用的高吸水性树脂几乎都是聚丙烯酸类，但其生物分解性差，易造成地下水及土壤环境污染，可生物降解高吸水性树脂的制备引起了学术界极大的关注。

Two novel"black"dyes KRu〓(ttp-COOH= 4'-p-COOH-phenyl-2, 2', 6', 2-tpy) and trans-Ru (4, 4'-dcbpy)〓〓 were synthesized, their photophysical properties in solutions and photo-electric properties adsorbed on the surface of nanocrystalline TiO〓 were studied.

研究了两个"黑色"染料trans-Ru(4，4'-dcbpy)〓〓和KRu〓(ttp-COOH＝4'-p-COOH-phenyl-2，2'，6'，2-tpy)的光物理性质及其在纳晶TiO〓薄膜电极上的光电转换性质，二者在红光区的光谱响应范围均比cis-Ru(4，4'-dcbpy)〓〓的宽，能吸收大部分的太阳光，确实在长波区增加了光谱的敏感性。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失