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In order to reduce the non-specific binding between oligonucleotides and bases mutation caused by the complicate secondary structure of DNA and excessive PCR amplification, a frequently phenomenon in one-step gene synthesis, we used a two-step method including assembly PCR and digestion-ligation step to synthesis Aspergillus niger lipase gene lipA.

本研究主要针对一步法长片段基因合成中复杂结构的非特异性配对和过多的PCR引入碱基错配等问题，采用二步法(组装PCR和酶切-酶连)合成了黑曲霉脂肪酶基因lipA。

Because transient increases in choroidal thickness are followed by a prolonged decrease in synthesis of extracellular matrix molecules and a slowing of ocular elongation, and attempts to decouple the choroidal and scleral changes have largely failed, it seems that the thickening of the choroid may be mechanistically linked to the scleral synthesis of macromolecules, and thus may play an important role in the homeostatic control of eye growth, and, consequently, in the etiology of myopia and hyperopia.

由于细胞外基质分子合成持续减少以及眼轴变长减缓，脉络膜厚度瞬间增加，对脉络膜和巩膜变化的缓慢调节彻底失败，脉络膜增厚可能与巩膜合成大分子机械性联系在一起，因此它可能在眼生长稳态的控制方面发挥了重要作用，而且，也可能是近视和远视的病因。

The factors affecting synthesis of micaceous iron oxide were discussed.Secondly, basing on the preparation of micaceous iron oxide, the caft and the condition of synthesis micaceous iron oxide pearlescent pigment is studied.

其次，在制备出云母氧化铁的基础上得出了包膜制备云母氧化铁珠光颜料的工艺路线与条件，讨论了影响云母氧化铁珠光颜料的因素，摸索出了金红色，金黄色，紫色，银白色云母氧化铁的合成控制条件。

Linear dimerization of isoprene to form myrcene ;2. Synthesis of Unsaturated Cyclothioethers byCycloaddition Reactions of Myrcene;3. The traditional synthesis is by the way of Diels-Alder reaction of myrcene with acrolein over Lewis acid catalysts in organic solvent.

二烷基咪唑或烷基吡啶卤化物与ZnCl2 构成一类新型的具有Lewis酸性的离子液体，以其为催化剂和反应介质，通过月桂烯与丙烯醛的Diels Alder反应高区域选择性地合成了对位柑菁醛。

The synthesis of the NiFe〓O〓 spinel matrix material, the utility of grain composition of the spinel ingredients when synthesizing the inert anode, and the synthesis of the inert anode was studied essentially in this thesis.

本文重点研究了镍铁尖晶石原料的合成，合成惰性阳极制品时原料的粒度配比和惰性阳极制品的合成。

This review on organobismuth chemistry is carried out as the following four aspects:(1) the molecular structure characteristics and synthesis of new organobismuth compounds;(2) as reagents for cross-coupling, oxidation, arylation, and other reactions;(3) as catalysts for organic synthesis;(4) as medicines for the treatment of ulcers, anti-tumor and radio-therapy, etc.

本文回顾了近10多年来有机铋化学的研究状况，主要从以下4个方面进行综述：(1)新型有机铋化合物的合成途径与结构特征；(2)有机铋化合物作为交联偶合、氧化、芳基化和其它反应的试剂；(3)作为催化剂；(4)作为生物医药用于溃疡、肿瘤和放射治疗等。

Products have good thermal stability anddecomposition temperature above 200℃; Selected lauryl amine as a case, then optimizedthe synthesis conditions by orthometric trial, and obtained the best synthesis technichs: themolar ratio of 1: 2.1, the reaction temperature was controlled at 80℃and the reaction timeof 16 h, reaction to the solvent n-propanol.

并对其热稳定性进行了分析，合成产物具有较好的热稳定性，分解温度都在200℃以上；以十二胺为例，用正交实验法优化合成条件，获得最佳合成工艺为：摩尔配比为1：2.1，反应温度控制在80℃，反应时间为16h，反应所用的溶剂为正丙醇。

the fibroblasts are activated in collagen synthesis in active keloid. The enhanced collagen synthesis by fibroblasts is a critical factor leading to the overabundant collagen accumulation in keloid.

结论在活跃增生的瘢痕疙瘩中，成纤维细胞胶原合成功能处于活化状态，胶原合成增加是瘢痕疙瘩中胶原过度积聚的重要原因。

Disclosed is a semi-aromatic polyamide containing oxazole ring and the related synthesis method, relating to a semi-aromatic nylon and the synthesis method.

一种含唑环半芳香聚酰胺及其合成方法，它涉及一种半芳香族尼龙及其合成方法。

Owing to the versatile application of pentaerythritol derivatives to organic synthesis, a lot of organic synthesis methods are able to be used in preparation of the new kind of glycoconjugate.

同时， 由于季戊四醇衍生物在有机合成领域应用广泛，很多有机合成方法都可以应用到这类糖缀合物的合成中。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失