查询词典 synchronized

Study on the total RNA content change and cell growth of Chlamydomonas reinhardtii after synchronized culture.

为了探索莱茵衣藻经光暗同步化培养后的细胞生长和总RNA的变化规律。

Methods and steps: With a Mega speed 25k high-speed cinecamera (100Hz), a AMTI force platform (1000Hz) and Biovision EMG (1000Hz) to capture methods were synchronized by the action to come to a complete stop.

方法与步骤：以一部Mega speed 25k 高速摄影机（100Hz）、一台AMTI测力板（1000Hz）与Biovision肌电仪（1000Hz）以同步方法撷取受试者由坐到站之完整动作。

This is a form of group skating that is less structured than synchronized skating and allows the use of props and theatrical costuming.

这种团队冰上项目与冰上列队相比，对队形的要求较低，并且允许运用小道具和话剧服装。

Result The parasites synchronized with sorbitol in vitro was treated by daphnetin and daphnetin-Fe complex.

经瑞香素（100μmol／L）作用1、2、3和4h后RNR活性分别被抑制7％、51％、69％和75％；在瑞香素浓度为0.1、1、100和1000μmol／L，作用6h后，RNR活性分别被抑制3％、31％、58％和93％；而瑞香素-Fe复合物作用6h后RNR活性分别被抑制8％、6％、11％和9％。

Objective To understand synchronized relation between rhythms at different function state and states shifted during epileptiform discharges.

目的 了解痫样放电不同阶段脑电信号各节律的同步关系及其在不同脑功能状态间转换时的变化。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Firstly, G. lamblia cells was synchronized. Then, using EM, Western bloting lamina and lamin were studied during the nuclear division.

首先进行了贾第虫的同步化，然后利用电镜、电泳和Western blot 技术对其核纤层在核分裂过程中的行为表现进行了研究。

Is ready to launch its campaign goal of 99 400 people, will be present in nearby areas experiencing outbreaks of meningococcal disease in Uganda synchronized movement.

正在准备开展以99 400人为目标的运动，将与目前在临近地区正经历脑膜炎球菌病暴发的乌干达同步开展运动。

The working principle of the deployment control mechanism of appendages is investigated in detail, the feedback control model is established. And the effect of parameters to synchronized motion is discussed, in addition the nonsynchronous motion is investigated through the analysis of the principle for deployment control mechanism and driving mechanism and simulation.

针对在工程实际中发现的帆板展开运动不同步现象，本文详细分析了最常见的附件展开控制机构—CCL的工作机理，建立了CCL的反馈控制模型，并给出其具体的数学表达式，讨论了机构参数对帆板运动同步性的影响，进而通过对展开控制机构和展开驱动机构的机理分析和数值模拟讨论了帆板的不同步现象，并提出了减小不同步量的策略。

Moreover,the mechanismunderlying the transition to nonlocal synchronization has been made clear,i.e.,that the oscillators between the nonlocal synchronized sites can transferthe synchronous components,although they stay in nonsynchronous status.

针对这一奇怪的时空动力学行为，我们详细研究了其机制，发现处于非局域混沌相同步中间位置的振子虽然没被锁相，但它们的运动中包含有相同步的元素起到了传递相同步信号的作用。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失