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They have used Franko Lin and Wilkins's judgment, and supplemented: The phosphoric acid radical constitutes more than two nucleotide chain's skeleton in the screw flank, the direction is opposite; Basic group in spiral inside, 22 correspondences.

他们采用了富兰克琳和威尔金斯的判断，并加以补充：磷酸根在螺旋的外侧构成两条多核苷酸链的骨架，方向相反；碱基在螺旋内侧，两两对应。

"Despite the extensive use of prebiotic-supplemented formulas in Japan and their recent addition to formulas in Europe, few safety studies have been published," explained lead author Frances Boyle.

第一作者Frances Boyle解释：无论是在日本的这类益生菌补充食谱还是最近在欧洲的其它食谱中，这类果寡糖虽然广泛应用，但是却没有相关的安全研究。

The asymbiotic germination indicated that the medium Hyponex No.1 was optimum for PLB formation when supplemented with potato juice, and optimum for PLB differentiation when supplemented with banana juice. Fresh sprouts were induced from the explant of the shoot tip cultured on the MS medium in the presence of BA 3.0 mg/L and NAA 0.2 mg/L.

结果表明：在花宝1号附加土豆的培养基上萌芽效果最好，原球茎发生率较高；分化阶段以花宝1号附加香蕉的培养基分化效果最理想，幼嫩茎尖在MS基本培养基上附加BA 3.0 mg/L与NAA 0.2 mg/L上可诱导出新芽。

Results When the culture medium was supplemented with 0 .05μg/ml sodium selenite,the proliferation of transformed cells was significant ly promoted and the transformation did not change remarkably,when supplemented w ith 0.25μg/ml,the proliferation was promoted but the transformation was inh ibited,and when with 0.75μg/ml and 1.50μg/ml,the proliferation was not pro moted and the transformation was strongly inhibited.Big dose of (1.50μg/ml) sodium selenite m ade the nuclei of the untransformed lymphocytes gather together like a few grape s doing,and even caused them to fuse.

结果 在培养物中添加0.05μg/ml亚硒酸钠能显著促进转化细胞增殖；添加0.25μg/ml可促进转化细胞增殖，但显著抑制细胞转化；添加0.75μg/ml和1.50μg/ml，不仅不能促进细胞增殖，而且强烈抑制细胞转化。1.50μg/ml剂量的亚硒酸钠使非转化淋巴细胞发生葡萄状聚集甚至核融合。

The optimal way for inducing floral buds is to preculture the plantets on 1/2MS medium supplemented with 0.5mg/L ABA and 0.5mg/L PP333 for 35 days, then transferred them onto 1/2MS medium supplemented with 1.0mg/L PP333 and 0.1mg/L TDZ. The frequency of floral buds induction increased sharply reaching 62.2% during a period of 150 days. All the mediums were added to 30g/L sucrose and 9g/Lagar.

通过筛选，诱导金钗石斛花芽形成效果最好的培养方法是：以1／2MS附加蔗糖30g／L，琼脂9g／L，活性炭0.5g／L为基本培养基(pH5.6～5.8)，将组培苗在加有PP_(333)0.5mg／L+ABA0.5mg／L的培养基上预培养35d后，再转入到附加TDZ0.1mg／L+PP_(333)1.0mg／L的培养基中。150d内统计的花芽形成率高达62.2％。

Supplemented with 5% coconut milk or 0.2% tryptone in the medium markedly promoted the hypocotyl callus growth, while 0.2% yeast extract had no important effect. Supplemented with 10% coconut milk, 2% tryptone or 2% yeast extract all inhibited the hypocotyl callus growth.

添加5％椰子乳和0.2％胰蛋白胨显著地促进生长，添加0.2％酵母提取液则未表现出显著的促进作用，而10％椰子乳、2％胰蛋白胨和2％酵母提取液均抑制愈伤组织的生长。

Methods Neomycin was used to destroy guinea pig utricular hair cells in vitro.The culture in experimental groups was supplemented with bFGF.The culture in control group was not supplemented with bFGF.All utricles were observed by scanning electronic microscope,and the number of survival utricular hair cells was accounted.

采用离体的豚鼠椭圆囊培养法，对照组为普通前庭培养液培养，实验组按培养液中含和不含bFGF分组，用新霉素造成各实验组培养的椭圆囊毛细胞损伤，各组椭圆囊均行扫描电镜检查并行毛细胞计数。

The results showed that: a great quantity of compacted callus were induced on the MS medium supplemented with 6-BA and a large amounts of corms and young seedlings were differentiated on the differentiation medium, but plenty of loosened callus on the MS medium supplemented with 2,4-D and limited young seedlings on the differentiation medium.

结果表明：在附加6-BA的MS培养基上，外植体可诱导出大量的致密愈伤组织，而且致密愈伤组织在分化培养基中能分化产生大量圆球茎和幼苗；而在附加2，4-D的MS培养基中，外植体被诱导出疏松愈伤组织，在随后的分化培养基上只能产生数量有限的幼苗。

The research on the tissue culture and cell suspension culture showed that thesuitable culture medium for inducing bud was MS supplemented with 1.5mg/L 6-BAand 0.1mg/L NAA, and for the generation-continuing multiplication was MSsupplemented with 1.5mg/L 6-BA and 0.2mg/L NAA. The rooting medium was1/2MS supplemented with 0.5mg/L IBA and the rooting rate was 45.0%. Plantletsurvival after transfer to sand was 52.5%.The induction rate of calli was66.7%～86.6% and the optimum medium was MS medium with 0.5mg/L 6-BA and2.0mg/L 2,4-D. The calli became smallest partical size, friable and had gooddispersion ability after 3 times successive transfer culture, the optimum medium wasMS medium with 0.2mg/L 6-BA and 2.0mg/L 2,4-D. Culturing these particles on sixkinds of MS liquid media with different hormone contents, the optimum medium wasselected basing on he change of the density of single-cell, the density of cellaggrefate and the mass of cell.

对蒜头果进行的组织培养与细胞悬浮培养研究结果表明：MS+6-BA1.5mg／L+NAA0.1mg／L+蔗糖3％激素组合能够较好地诱导芽的初始分化和增殖，适宜的芽苗继代增殖培养基为MS+6-BA1.5mg／L+NAA0.2mg／L+蔗糖3％；采用1／2MS+IBA0.5mg／L+蔗糖3％为生根培养基，生根率为45.0％；移栽到河沙的生根苗成活率为52.5％；愈伤组织诱导率为66.7％～86.6％，其中以MS+6-BA0.5mg／L+2，4-D2.0mg／L+蔗糖3％的培养基最佳，其诱导出的愈伤组织具有较强的增殖能力和较好的脆散结构，最佳继代培养基为MS+6-BA0.2mg／L+2，4-D2.0mg／L+蔗糖3％，且培养基中的6-BA与2，4-D浓度的比值越小，愈伤组织生长越快，结构越脆散，增殖率越高；将继代后的愈伤组织转入6种含不同激素浓度组合的MS液体培养基中进行振荡培养，在综合分析各培养基的单细胞密度，细胞团块密度，细胞生物量增长率等指标后，初步筛选出MS+6-BA0.2mg／L+2，4-D2.0mg／L培养基为较好的液体培养基。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。