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Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

The results showed that the four treatments of cytochrome c supplemented with ATP all suppressed the pollen germination and pollen tube growth, and induced nuclear DNA degradation, different treatments with different ratios of double nuclei, single nucleus, and no nuclei. Treatment with heavy concentration of ATP also restrained pollen germination and pollen tube growth, but can not induced nuclear DNA degration.

结果表明，细胞色素c和ATP混合处理的四个组合都能抑制离体花粉萌发和花粉管生长，并且引起了花粉管核DNA的降解，不同处理中双核、单核及无核花粉管所占比率存在明显差异；高浓度ATP虽能抑制花粉萌发和花粉管生长，但不能引起花粉管核DNA的降解。

This kind of food is not very nutritious so it has to be supplemented with vitamins and minerals.

这种食物不太有营养的，所以它要加以补充维生素和矿物质。

The producers of optical disk formatblu-ray was supplemented by new representative.

制作光盘格式的蓝光进行了补充新的代表。

Legally each year - supplemented by an additional 300,000 who enter illegally or overstay their visas every year.

此外，每年会有30万非法入境和签证过期后仍逗留在美国的非法移民。

The percentage of root biomass allocated to cluster roots increased in the soil layer supplemented with P in a split-root system.

在分根系统中，白羽扇豆的排根占根系干重的比例在局部供磷的土层中大量增加。

It is supplemented by an extensive Pahlavi literature, consisting in part of translations from the sacred canon and in part of original matter.

这是辅之以广泛巴列维文学，组成部分的翻译从神圣佳能和部分原始问题。

Paulinus at York were Benedictine pioneers, and their labours were afterwards supplemented by other monks who, though not strictly Benedictine, were at least assisted by the black monks in establishing the Faith.

圣奥古斯丁和圣劳伦斯在坎特伯雷，圣贾斯特斯在罗切斯特，圣糖尿病在伦敦，和圣Paulinus在纽约被本笃先锋，他们的辛勤劳动是事后辅之以其他僧侣谁，但没有严格笃，上协助最不发达国家的黑色僧侣在建立信仰。

The correlation of economics and pedagogics to educational economics is discussed in this part. The educational economics" methods are mainly determined by economics, which are restricted and supplemented by pedagogics at the same time. It is especially pointed out that how pedagogics" notion, ethic, concept system and so on are embodied internally in educational economics research.

分析论述了经济学和教育学的研究方法及理论观念与教育经济学研究的相互关系，提出经济学方法对教育经济学方法的决定性，以及教育学对于教育经济学方法的制约和补充，特别是教育学的观念、伦理、概念体系等应该如何在教育经济学的研究中内在地体现出来。

Based on the established optimal tissure culture system for pelargonium citrosum Vanleenii,the different concentrations of Ca~(2+) were supplemented into the media to test their effects on prolifertion and rooting of pelargonium citrosum Vanleenii.

在已经建立的驱蚊香草试管苗快速繁殖体系基础上，在已经筛选出的最佳增殖和生根培养基中添加不同浓度的Ca2+进行增殖和生根试验。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。