查询词典 supernatant

Methods: The total polysaccharide was extracted with distilled water from mycelia of paecilomyces cicadae. The colorimetric MTT was used to analyze the proliferative activity of PBMC, U937 and K562 which were treated with PCPS of 7 concentrations respectively in vitro. ELISA was used to detect the content of hTNF-α, hIFN-γ from supernatant of culturing PBMC that was treated with PCPS for 44 h.

用水提取蝉拟青霉菌丝体总多糖，以MTT法测定不同浓度总多糖对人外周血单个核细胞及白血病细胞株U937、K562增殖的影响，ELISA法测定多糖作用44h后，外周血单个核细胞培养上清液中hTNF-α、hIFN-γ的含量。

Strains could inhibit conidium germination, 5 strains made the pathogen grow vegetably and 9 strains inhibited its tube growth.M.grisea produce several swollen cells on the sites of the appressorium differentation and lost the ability to infect onion epidermis treated with the culture supernatant of h23 strain.

结果如下：获得21株对稻瘟病菌附着胞形成具有拮抗作用细菌菌株，其中3株诱导孢子在附着胞分化位点产生囊状物；其中4株抑制孢子萌发；5株使孢子呈营养生长；9株抑制芽管生长。

It is reported in this study that (1) two high enzyme-yeilding strains TK54 and T12 have been screened from four choleterol-secrecting experimental strains, he supernatant of which have been tested for insecticidal activity with the results showing that cholosterol oxidase can strongly inhibit the vigor of cotton boll worm.

本研究从4个分泌胆固醇氧化酶的实验菌株中，筛选出两个高产酶菌株TK54和T12，用其上清进行杀虫实验发现，胆固醇氧化酶具有较强的抑制棉铃虫生长的活性。

Objective\ This study was to investigate the effect of crocidolite fibe rs on IL-8 protein and mRNA levels in A549 cells cultured supernatant and the r o le of tyrosine kinase inhibitor PD98059 on IL-8 expression.

目的 研究青石棉纤维作用于A5 4 9细胞后IL 8蛋白和基因表达水平及酪氨酸激酶抑制剂PD980 5 9对IL 8表达的影响。

Decant the supernatant liquid from the solid as far as possible.

尽可能快地把固体上层的清夜倾滗掉。

You centrifuge the solution, decant the supernatant, and discard the pellet .

你然后对溶液离心，慢慢倒出上清，弃沉淀。

After adding extraneous H〓O〓 at the concentration of 100μM, the culture supernatant with Lip activity could decolor the dyes rapidly.

加入100μM的外源H〓O〓，含过氧化物酶活力的酶液可以使染料迅速脱色。

A recombinant protein of Bla g 2 has been obtained, which is soluble in the supernatant and therefore can avoid a process of denaturalization and renaturation of the recombinant.

获得了真核表达的德国小蠊主要变应原重组蛋白Bla g 2，该蛋白以可溶性蛋白的形式分泌到培养液中，避免了原核表达的变复性过程。

PartⅡTransform the yeast, screen the excellent expression yeast strain, induce the yeast to express the fusion protein and depurate the target protein. The recombination plasmid was transformed into the yeast GS115 successfully by means of electrotransformation. The excellent yeast strain was got by G418 screening. We achived the yeast supernatant including target protein induced by methanol.

第二部分：酵母的转化、蛋白高表达菌株的筛选、重组蛋白的诱导表达及纯化实验利用电转化的方法，成功地将重组质粒pPIC9k-PTD-hA20导入毕赤酵母GS115，并通过G418浓度梯度筛选得到了蛋白高表达菌株，并对蛋白高表达菌株进行甲醇诱导表达，得到了含有目的蛋白的酵母上清液。

Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly. Between the 60th and 72th hour, many oocysts emerged. Inoculated by the C. parvum -infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.

在感染后72 h内，隐孢子虫出现连续发育阶段，包括脱囊、子孢子、裂殖子、裂殖体、滋养体、配子体、合子、薄壁卵囊和厚壁卵囊，在60～72 h内形成卵囊；用感染48 h的细胞培养上清接种于免疫抑制小鼠， 10 d后有隐孢子虫卵囊排出。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。