查询词典 supernatant

The analysis of the samples collected from the wastewater treatment plant showed that the recycled supernatant had a pH of 7.16~8.21, a conductivity of 371.1~769.1 μmho/cm, a total dissolved solid of 193.3~399.9 mg/L, and a turbidity of 0.901~54.3 NTU. The suspended solids of the recycled supernatant was found to be 0.4~45.6 mg/L, lower than the standard value in the Effluent Standards (50 mg/L). The ammonia nitrogen (NH3-N) and the Total Organic Carbon of the recycled supernatant were found to be 0.06~1.5 mg/L and 1.533~17.437 mg/L, respectively. The analysis of the Chemical Oxygen Demand of the wastewater treatment plant showed a concentration of 12.1~128.5 mg/L in the sample of the recycled supernatant.

在废水处理单元采样分析结果方面，回收之上澄液之pH范围为7.168.21间、导电度值范围在371.1769.1 μmho/cm间、总溶解固体量范围在193.3399.9 mg/L间、浊度范围则在0.90154.3 NTU间；在悬浮固体方面，其回收上澄液之范围在0.445.6 mg/L间，且回收水之悬浮固体均小於放流水水质法规标准值50 mg/L；在氨氮（NH3-N）方面，回收上澄液之范围在0.061.5 mg/L间；在总有机碳部份，回收上澄液之浓度范围在1.53317.437 mg/L间；而在净水场废水处理单元化学需氧量分析结果上，可发现净水场在回收上澄液之样本部份其浓度在12.1128.5 mg/L间。

Results (1) the laminin synthesis was significantly improved by PBMC from nontreatment group (P＜0.05);(2) the 3 H-prolin incorporation was significantl decrease in the GEC treated by PBMC supernatant from nontreatment group(P＜0.05);(3) the supernatant of PBMC from nontreatment group stimulated the collagen Ⅲ synthesis(P＜0.05), while, there was no significant difference of collagen Ⅳ synthesis between 4 groups;(4) the activity of PBMC supernatant have no close relation with urinary protein excretion, pathological type and clinical type.

结果 (1)肾病极期未经激素治疗组PBMC上清明显促进了GEC合成层粘连蛋白；(2)未治PBMC上清抑制了GEC合成总胶原；(3)未治组PBMC上清促进了Ⅲ型前胶原的合成，而对Ⅳ型胶原的合成无明显影响；(4)肾病患儿PBMC的上述作用与是否足量激素治疗有关，而与尿蛋白能否阴转、肾组织病理类型、肾病临床类型等无直线相关关系。

Methods Sputum sample of 146 patients with tuberculosis are examined by smear, Bactec detection and culture on acidic Rose Medium, with the supernatant, the middle-supernatant, the supernatant of self-sedimentation and centrifugal precipitate in sample tubes after treatment of 2%NaOH.

对146例结核病人的痰标本分别作涂片、Bactec 法检测和 2%NaOH 碱处理后，吸取标本管内上清液体、中部液体、自沉淀液体和离心沉淀法后的标本接种于酸罗氏培养基。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

The invention discloses an InP quantum point preparing method, comprising the steps of:(1) mixing InCl3 with trioctylphosphine oxide, and preserving heat at 90-110DEG C to prepare a solution whose In content is 0.1-0.3mol/L;(2) raising temperature to 130-180DEG C, and charging argon gas;(3) injecting PSi(CH333 into InCl3-TOPO compound in the molar ratio of 1 to 1-1 to 2;(4) when the solution color turns into transparent red or orange, raising temperature to 260-270DEG C and preserving heat;(5) lowering temperature to 90-110DEG C and injecting dodecyl amine, decyl amine or mercaptan;(6) dissolving reacting mixture in nonpolar solvent to form transparent colloidal solution, then adding in polar solution until the colloidal solution is muddy, and centrifugally separating and obtaining deposits and supernatant; and (7) centrifugally separating the supernatant, where the above steps (1)-(5) are performed.

本发明公开了一种InP量子点的制备方法，其步骤为：①将InCl 3 与三辛基氧膦混合，在90～110℃保温制得In含量为0.1～0.3mol/L的溶液；②升温到130～180℃，通入氩气；③将PSi(CH 3 3 3 注射入InCl 3 -TOPO复合物中，其摩尔比为1∶1～1∶2；④待溶液颜色变为透明的红色或橙红色，将升温260～270℃保温；⑤降温到90～110℃，注入十二胺、癸胺或硫醇；⑥将反应混合物溶于非极性溶剂，形成透明的胶体溶液，然后加入极性溶剂，直至胶体溶液出现混浊状，然后离心分离获得沉积物和上层清液；⑦取上层清液进行离心分离。上述步骤①～⑤在保护气氛下进行。

Furfur. At the same time, selected the culture supernatant. Used the whole-fat-cow"s-milk plate method, the Folin-phenol method and the QuantiCleave proteinase Assay Kit to test the proteinase activity of mycelial phase and yeast phase of M. furfur. The effect of temperature and pH on this assay was also evaluated.Result:①The whole-fat-cow"s-milk plate method: Seven days after inoculation, the proteinase activity of the mycelial phase supernatant (12.5 ± 1.2mm) is higher than that of the yeast phase (9.25±0.71mm)(p.05); With the passage of the inoculation time, the proteinase activity of the mycelial phase is rising; the temperature has no obvious effect on the proteinase

结果：①全脂牛奶平板法：培养第7天菌丝相上清液蛋白酶活性(12.5±1.2mm)高于酵母相(9.25±0.71mm)(p＜0.05)；菌丝相上清液蛋白酶活性随培养天数延长而增大；25℃和37℃两种温度对上清液蛋白酶活性无明显影响(P＞0.05)；②Folin-酚法：上清液蛋白酶活性随培养天数、菌落计数、菌丝诱导率增加而增大，培养液pH值呈下降趋势；同一天的菌丝相上清液蛋白酶活性明显高于酵母相(p＜0.05)；三种温度(25℃、37℃、49℃)及三种pH值(3.8、5.6、7.4)对上清液蛋白酶活性无明显影响；温度37℃、pH5.6时菌丝相上清液蛋白酶活性最高；③QuantiCleave蛋白酶试剂盒：菌丝相及酵母相上清液中均含有蛋白酶且

Results: The homogeneous enzyme was obtained on a single step by affinity chromatography from the supernatant of transformed cells, and its reductive activity of menadione was 18.6-fold of the supernatant of the empty-transformed cells and 7.3-fold of transformed cells.

结果：诱导表达后的细菌裂解上清液经一步亲和层析后得到单一酶蛋白，其对K3的比活性分别是原空白菌裂解上清液和经诱导表达后的菌裂解上清液的比活性的18.6和7.3倍。

Results Multidrug resistance could be detected in the tumor tissue after the injection of retrovirus around and into it.After filtration and centrifugalization,the concentrated supernatant was 2 times efficient than the original supernatant and P-GP expression was detected in 60% of the tumor cells.mdr1 mRNA expression in the tumor tissue was significantly increased after gene transfer as detected by RT-PCR.

结果 携带mdr1基因的逆转录病毒上清液注射后肿瘤模型产生多药耐药性，经过滤和离心浓缩的病毒上清液60%细胞表达P-GP，是病毒原液基因转移率的2倍；RT-PCR表明基因转移后，肿瘤组织内mdr1 mRNA的表达量明显增加。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

The culture filtrate of strain LN-176 was tested for light absorption at 260nm and 280nm, and the results showed that the protein content in the culture filtrate significantly increased. After ammonium sulphate precipitation, the supernatant and the precipitation were separated to testify their antagonistic activity, and it was found that the precipitation was inhibitory to Ustilago hordeum , but the supernatant not.

LN-176菌株的发酵液在260nm和280nm下进行吸光度测定，结果表明发酵液中蛋白质含量明显升高；发酵液经硫酸铵盐析后，取上清液和沉淀物分别做抗菌试验，发现沉淀物对青稞散黑穗病菌有拮抗活性而上清液无活性。

" I' d like to get some rough idea about music in the baroque ear, please."

请简要介绍一下巴罗克时期的音乐，好吗？

The results showed that the peak latency and pattern of SEPs elicited by electrical needling in LI-l1 and MP were similar. The amplitude of SEPs elicited by electrical needling in LI-l1 was higher than that of MP. There was no obvious SEPs generation when MM was electrical needling.

结果显示，电针刺激曲池穴和其邻近桡侧伸腕长肌运动点诱发性脑电波的波形和波峰时间是类似的，只是穴位的诱发性脑电波波幅较大，而电针刺激非穴位点并没有明显的诱发性脑电位。