查询词典 sulphur chloride

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Objective To evaluate the safety and efficacy of strontium-89-chloride for management of bone metastases in patients without bone pain.

目的 评价89SrCl2治疗无骨痛的多发性骨转移瘤的疗效和安全性。

This functioned as a selective bridge, permitting the passage of positively charged sodium ions, but not negatively charged chloride ions.

它可以作为有选择的桥梁让带正电的钠离子通过，同时阻止了带负电的氯离子。

Next, Dr LaVan and his colleagues installed a protein called alpha-hemolysin into the protocell membrane. This functioned as a selective bridge, permitting the passage of positively charged sodium ions, but not negatively charged chloride ions.

接下来，拉文和他的同事们在原细胞的细胞膜中加入了一种叫阿尔法溶血素的蛋白质，这样就建立了一个选择性的通道——允许正极的钠离子通过，却阻止负极的氯离子。

The instrument can be used for the determination of total sulfur, total chrorine, trace water, bromine number and bromine index, mercaptan and hydrogen sulfide, chloride ion, salt content and chemical oxygen demand.

仪器可广泛用于各种样品中总硫、总氯、微量水分、溴价和溴指数、硫醇和硫化氢、氯离子和盐含量以及化学需氧量等的测定。

The main contents and innovation points of this thesis are as follows: First, based on the reaction of using vitriol and potassium chloride to get calcium sulphate, we studied the influence of different fields, especially microwave, on the crystallization of CaSO_4. Through special designed device, the same temperature rising has been achieved in microwave heating and traditional water bath.

本文的主要研究内容和创新点如下：第一，本文基于硫酸与氯化钙反应生成硫酸钙反应，研究了不同物理场对硫酸钙结晶的影响，重点探讨了微波对硫酸钙结晶的影响，在相同温升速率下，从相同初温加热到相同末温，微波加热使硫酸钙晶体形貌发生了很大改变，晶体粒度明显变小。

This Technology adopts green chemical product---Dimethyl Carbonate to substitute carbonyl chloride and exchange with Phenolic Esters to produce Diphenyl Carbonate.

本技术用绿色化工产品碳酸二甲酯代替光气与苯酚酯交换生产碳酸二苯酯，是一条清洁生产工艺。

The invention is characterized in low check limit, high sensitivity, repeat application, and wide check air range, and the invention can be used to check various volatile harmful gas, more particularly check the discharge and leaked harmful gas as NO2, SO2, NO, CO, H2S and carbonyl chloride.

由于本发明传感器具有检测限低，灵敏度高，可重复使用，检测气体范围广等特点，故本发明可广泛用于检测人类生产生活环境中的各种挥发性有毒气体，特别适用于工业生产中NO 2 、SO 2 、NO、CO、H 2 S，及光气等有害气体排放合格性的检测及泄漏的检测。

Meso-Tetrakis(2,6-dichlorophenyl)porphyrinmanganese chloride and carbonyl ruthenium meso-tetrakis(2,6-dichlorophenyl)porphyrin were prepared and used to catalyze the diastereoselective epoxidation and amidation of 3β-substituted Δ 5-steroid derivatives.

研究了 5 ，10 ，15 ，2 0 (2 ，6 二氯苯基)卟啉锰及其钌配合物这类非手性的催化剂催化3β取代的Δ5 甾体衍生物的非对映选择性环氧化及酰胺化反应。

Castor seeds were degreased with 3 kinds of solvents including petroleum ether, aether and methylene chloride, and ricins were extracted from the defatted castor cake with acetic acid solution. And the rat-killing activities of 3 kinds of ricins were compared in the rat-killing activity test.

方法］用石油醚、乙醚、二氯甲烷3种溶剂分别对蓖麻粗籽进行脱脂后，用醋酸水溶液从脱脂的蓖麻饼中提取蓖麻毒蛋白，通过杀鼠活性试验比较3种蓖麻粗毒蛋白的杀鼠活性。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。