查询词典 sulfur grundum
- 与 sulfur grundum 相关的网络例句 [注:此内容来源于网络,仅供参考]
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Keywords Palladium;Titanium oxide;Barium oxide;Aluminum oxide;Nitrogen oxide;Storage-reduction catalyst;Sulfur resistance;Lean burn engine emission
钯; 氧化钛;氧化钡;氧化铝;氮氧化物;储存-还原催化剂;抗硫性能;贫燃发动机尾气
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Base plate shall be produced by a low sulfur and low phosphorus refining process.
底盘的生产要经过低硫、低磷化处理。
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The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.
(1)本研究用RT-PCR方法,从人组织细胞总RNA中扩增可溶性TWEAK胞外区(sTWEAK1和sTWEAK2)的cDNA序列及全长编码序列,用琼脂糖凝胶电泳分析PCR产物,胶回收目的基因片段,连接到pMD18-T克隆载体中,转化大肠杆菌K802,PCR和酶切筛选阳性克隆,全自动DNA测序验证序列;(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中,分别转化大肠杆菌BL21和TB1,PCR筛选和酶切鉴定,阳性克隆用IPTG诱导表达,表达产物用SDS-PAGE分析和Western blot验证融合蛋白;(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白;(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测,贴壁细胞用结晶紫染色法,悬浮细胞用磺酰罗丹明B染色法,酶标仪检测OD值;(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量;(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况;(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响;(8)用双荧光素酶报告基因检测法,测定表达产物对敏感细胞NF-κB的影响;(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上,用脂质体转染法转染HEK293细胞,PCR鉴定重组质粒。
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This design is mainly on sintering flue gas emissions of sulfur dioxide total control of the main purpose is to design suitable to China's national conditions of the technologically advanced and economically reasonable sintering and other industrial boiler flue gas desulphurization new technology and new equipment.
摘要本设计主要是对烧结烟气排放二氧化硫总量控制方法的研究,主要目的是设计出适合我国国情的技术上先进、经济上合理的烧结和其他工业锅炉烟气脱硫新工艺和新设备。
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Fresh edible fungi and seaweeds, vegetables in brine sauce and steamed bread were main contributions of dietary exposure of sulfur dioxide for the populations in countryside.
但是城市和农村略有差别,城市人群主要以干制食用菌和坚果与籽类为主,农村人群则以新鲜的食用菌、盐渍的蔬菜和馒头等为主。
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The instrument can be used for the determination of total sulfur, total chrorine, trace water, bromine number and bromine index, mercaptan and hydrogen sulfide, chloride ion, salt content and chemical oxygen demand.
仪器可广泛用于各种样品中总硫、总氯、微量水分、溴价和溴指数、硫醇和硫化氢、氯离子和盐含量以及化学需氧量等的测定。
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Hot Springs has a very rich mineral resources of temperature, water temperature as high as 76 ° C, spring water is rich in iron, sulfur, radon, calcium, magnesium and other 48 kinds of minerals wholesome for nerve pain, rheumatism , lumbar muscle strain, muscle atrophy, gastroenterology and many other diseases with significant efficacy;泡浴can often skin beauty, beauty fitness.
龙门温泉有十分丰富的温矿泉水资源,水温高达76°C,泉水富含铁、硫、氡、钙、镁等48种有益身心的矿物质元素,对神经性骨痛、风湿病、腰肌劳损、肌肉萎缩、肠胃病等多种疾病有显著疗效;经常泡浴能护肤美容、养颜健身。
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The Company to sell the ancient Malone, stearic acid, zinc oxide, antioxidant, accelerator, sulfur, carbon black, flame retardants and other accessories mainly.
本公司以销售古马龙,硬脂酸,氧化锌,防老剂,促进剂,硫磺,碳黑,阻燃剂等辅料为主。
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With regard to desulfuration inside chain conveyer type furnace, a production line of sulfur capture briquette has been designed which has met the requ of desulfuration through test, production and practice to saves energy.
针对链式炉炉内脱硫,设计了固硫型煤生产线,通过测试、验、产和实际使用,使固硫型煤满足脱硫要求,同时,节能效果也较明显。
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Its original purpose is fuel, because of flammable charcoal, fire, ash less, non-sulfur content, etc., so has always been a high-quality fuel.
它的原始用途是燃料,由于木炭易燃、耐燃、灰分少、不含硫等特点,所以一直是一种优质的燃料。
- 相关中文对照歌词
- Sulfur
- Only Ashes
- A Mind Insane
- Splash, Turn, Twist
- Heartbreak Warfare
- Ghost Of Frankenstein
- Homunculus
- Rat Race
- Spring Can Really Hang You Up The Most
- Lazy Flies
- 推荐网络例句
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He paid $5,000 for this cupcake.
他我这个纸杯蛋糕花了5000美元。
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I mean, if you want to, that is
我的意思是,如果你们想吃的话。
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In addition to his fee, he would be awarded an unaltered clone of himself.
除了费用,他将获得一个不作任何改造的克隆体。