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The invention discloses a preparing method of bacillus alcaligenes strain Alcaligenes sp., CCTCC No.M206121 of degraded o-nitrophenol, which comprises the following steps: allocating inorganic salt culture medium with dibasic sodium phosphate, monobasic potassium phosphate, manganese sulfate, copperas, addex-magnesium, calcii chloridum, blue copperas, white copperas and hydrogen dioxide solution; adjusting pH value; sterilizing under high temperature; enriching bacterial; sampling from sludge; allocating suspending liquid with soil sample and inorganic salt; seeding into ortho-nitrophenol fresh organic salt culture medium with finite volume seeding quantity; keeping the temperature; culturing on shaker; getting enriched culture; separating and purifying enriched culture in LB solid culture medium.

本发明公开了一种降解邻硝基酚的产碱杆菌菌株及制备方法，产碱杆菌为Alcaligenes sp。，CCTCC No.M206121。其制备步骤是：首先是配制无机盐培养基，成分为磷酸氢二钠、磷酸二氢钾、硫酸锰、硫酸亚铁、硫酸镁、氯化钙、硫酸铜、硫酸锌和双蒸水，调pH值高压灭菌；其次是菌种的富集，从污泥中取样，将土样与无机盐培养基配制成悬浮液，将悬浮液以一定体积接种量接种到含邻硝基酚的新鲜无机盐培养基中，恒温，摇床培养，获富集培养物；第三是将富集培养物在LB固体培养基中分离和纯化。

The former mainly have collagen, coralline, aminoglucasan, chondro-itin-sulfate and fibrin glue.

天然材料主要有胶原、珊瑚、氨基葡聚糖、硫酸软骨素、纤维蛋白凝胶等。

The steps for preparing insecticide include: first shaking flask for fermentation, adding amidon, soya bean cake powder, cotton cake powder, protease leather, yeast powder, maize milk, magnesium sulfate heptahydrate, monobasic potassium phosphate, dibasic potassium phosphate, calcium carbonate alpha-amylase and make-up water by ratio in culture medium with 100 unit volumes; secondarily filling 100ml culture mediums in triangular bottle, then seeding BTH-13 after disinfection and culturing them until the brood-cell pulls off under finite temperature; third adopting the selected culture medium and fermenting them in full-automatic fermentation tank, acidifying the fermentation liquor after the fermentation, adding the emulsifier, and directly performing spray-drying after the compression of the fermentation liquor to prepare the wettable powder.

制备杀虫剂的步骤是：首先是摇瓶发酵，在100单位体积的培养基中，按比例加入淀粉、豆饼粉、棉饼子粉、蛋白胨、酵母粉、玉米浆、七水硫酸镁、磷酸二氢钾、磷酸氢二钾、碳酸钙、及α-淀粉酶，补充水；其次是在三角瓶中装100ml培养基，灭菌后接种BTH-13，在一定温度培养至芽孢脱落；第三是采用筛选出来的培养基，在全自动发酵罐中进行发酵，发酵结束后发酵液酸化，加入乳化剂，酵液浓缩后直接喷雾干燥制备可湿性粉剂。

Preservatives in common use include arsenic, copper sulfate and creosote etc.

常用防腐剂包括砷、硫酸铜和木馏油。

The color reaction of the cationic dye crystal violet, with anionic surfactant, sodium dodecyl sulfate was studied in this paper.

本文研究了碱性染料与阴离子表面活性剂十二烷基硫酸钠的显色反应。

Application of the material characteristics of the absorption spectra were studied under different conditions of crystal violet and the anionic surfactant sodium dodecyl sulfate to form ion-association complex of the purple of the optical properties.

应用物质的吸收光谱特性，研究了不同的条件下结晶紫与阴离子表面活性剂十二烷基磺酸钠形成的紫色离子缔合物的光学特性。

It was shown that upon addition of sodium dodecyl sulfate to solutions of crystal violet in the same concentration, making a series of solutions with graduation of concentration of SDS, a gradual decrease in absorbance at the abs.

于浓度相同的结晶紫溶液中，加入不同浓度的十二烷基硫酸钠组成一系列含有相同浓度CV和不同浓度SDS的混合溶液。

The influence of urea on the crystallization of calcium sulfate was studied in a MSMPR crystallizer.

以试剂磷酸二氢钙、硫酸和尿素为原料，模拟磷矿-硫酸脲反应中的硫酸钙结晶过程，在MSMPR结晶器中研究了尿素对硫酸钙结晶的影响。

The mechanism of made it by vacuum evaporation using DTB crystallizer is studied, and the detail of the technical flow is discussed. The factors which influence the granularity of anhydrous sodium sulfate are studied .

本论文主要讨论了用DTB型结晶器、采用真空蒸发结晶法制备较大颗粒硫酸钠的机理、具体的工艺流程以及各种因素对结晶粒度的影响。

Through the operation of this technology,not only the qualified cupric sulfate and copper oxide can be obtained,but also the recovery rates of gold and silver can...

通过该工艺的操作，不仅能制得合格的硫酸铜和氧化铜，并且能回收其中所含的金、银，回收率分别为961％和832％。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。