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Recently several years,the company is engaged in development of Halon substitute,and since year 1997 has successively succeeded in development of 5 kinds substitute,HFC227ea heptafluorpropane,HFC23 fluoroform,HFC236fa hexafluoropropane ,HCFC/A,HCFC/C.

近年来，我公司致力于哈龙替代品的开发，97年以来先后开发成功七氟丙烷、三氟甲烷、六氟丙烷、HCFC/A、HCFC/C五种替代品，形成千吨级生产规模，并已大批量出口海外。

A store Peking Man site locates the southwest building in Peking City mountain area a store main beam of boat mountain north, ising a material to enrich most in the world, most the human site of the earlier period in system, year in age.1921 In stone in old in and in worthy in most in the to 1927, the archeologist discovers outside" Peking Man" cave site three times successively three human tooths fossil,1929, discovers again Peking Man skull fossil, and artificial creation of tool with use the fire historic relic, the important cist that to become to frighten the world detection.A year a store site still discovers to be apart from to invite now summit of hill hole person's fossil a thousand yearseses ago with culture keepsake.

周口店北京人遗址位于北京市西南房山区周口店镇龙骨山北部，是世界上材料最丰富、最系统、最有价值的旧石器时代早期的人类遗址。1921年至1927年，考古学家先后三次在"北京人"洞穴遗址外发现三枚人类牙齿化石，1929年，又发现了北京人头盖骨化石，以及人工制作的工具和用火遗迹，遂成为震惊世界的重大考古发现。1930年在周口店遗址还发现距今约2万年前的山顶洞人化石和文化遗物。

Four groups of oligonucleotides were successively cloned in pMD18-T vector to obtain four large fragments. Ligation of these four fragments resulted in a cloned DNA MgE717 coding for the 29～268 amino acid region of gE. Sequencing result showed that there is a mutation at 255 amino acid, Asn instead of Asp.

分四段分别合成，基因拼接得到了改造后的基因片段MgE717，经测序，除了在gE基因+763位核苷酸处存在一碱基置换，导致第255位的氨基酸残基由Asp突变为Asn，其余的序列与PrV鄂A株gE基因的氨基酸序列相符。

A 717 base pairs long artificial gene fragment of PrV gE has been synthesized by using preferred codons in the highly expressed Pichia genes. Four groups of oligonucleotides were successively cloned in pMD18-T vector to obtain four large fragments.

通过人工合成基因的方法对编码gE蛋白的第29～268位氨基酸残基的核苷酸序列（717bp）进行改造，用P.pastoris高表达蛋白偏爱的密码子替换原有的核苷酸序列，氨基酸序列保持不变。

The center was the first engaged in propolis vaccine research and development for livestock and poultry in China, owned the most fruitful results of research and development, undertaked more than ten items of national, provincial and ministerial projects, and received eight items of science and technology achievement awards in which the fowl cholera propolis inactivative vaccine won the national third award, and successively obtained The New National Certificate of Veterinary Drugs, National New Product Certification, and National Invention Patent Certificate.

该中心是我国最早从事畜禽用蜂胶疫苗研究开发的单位，也是研究开发成果最为丰硕的单位，先后承担国家级、省级、部级有关蜂胶疫苗的研究开发项目十余项，获国家、省、地、科技成果奖8项，其中，发明的禽霍乱蜂胶灭活疫苗获国家发明三等奖，并先后获得国家级新兽药证书、国家级新产品证书、中国发明专利证书。

The world-level fowl cholera inactivated propolis vaccine has been awarded the national invention third prize and successively obtained National Invention Patent Certificate, National New Product Certification and The New National Certificate of Veterinary Drugs.

居世界领先水平的禽霍乱蜂胶灭活疫苗荣获国家发明三等奖，并先后获得国家发明专利证书、国家级新产品证书和国家级新兽药证书。

In this more than one year's time, FTC has successively carried on a series of cooperations with many enterprises, many schools.

在这一年多的时间里，台塑网先后和多家企业、多所学校进行了一系列的合作。

Successively with dataflow picture, data dictionary, function structure chart various kinds

在系统的设计中，详细的展现了系统的各个功能模块。

The system analyse varieties of data successively with the function chart, the E-R graphic and tables of database.

在系统分析中先后用系统的功能结构图、数据库的E-R图、数据库表分析了系统所需的各种数据。

By the method of subordinate function, the sequence of resistance from strong to weak successively was determined.

根据综合指标隶属函数运算，确定了供试番茄砧木抗南方根结线虫的顺序。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失