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The upstream regulatory region of the storage protein was separated from Glycine max variety "Ji Nong T7018" genomic DNA, according to published glycinin 11S G7 subunit (Gy7) gene(GenBank AF319776) sequence, a pair of primers was desgeined, in order to amplify the array before Gy7 gene initiation codon.

我们以大豆品种&吉农T7018&的基因组DNA为模板，根据已发表11S球蛋白G7亚基(Gy7)基因(GenBankAF319776)序列，设计一对引物，通过PCR扩增得到Gy7基因的启动子序列。

Herbicidal activities of selected sulfonylureas and the crystallization of monosulfuron with Arabidopsis thaliana AHAS catalytic subunit were studied.

随着当今环境问题加剧和人类可持续发展的需要，超高效低毒环保农药将更加受到世人的重视。

Subunit homodimers were also found existing in heteromeric NMDA receptors formed between NR1 and NR2 subunits.

我们的结果显示，当NR1a，NR2A，NR2B亚单位单独在HEK293细胞中表达时，可以各自形成同二聚体，当与其它亚单位共表达形成NMDA受体时，这些同聚体仍然存在。

Our results suggested that the channel contains two of each subunit and the stoichiometry is apt to 2:2 if hetermeric ASIC channel is a tetramer,while the assembly of ASICs is random,the stoichiometry is not fixed(2:1 and 1:2 coexist)if hetermeric ASIC channel is a trimer.This study provided interesting new data supporting the occurrence of the homomeric and heteromeric assembly of ASICs.

另外，我们还对ASIC异聚体的分子比情况进行了初步的探讨，结果发现如果所形成的ASICs异聚体通道是四聚体，那么每种亚单位有两个，分子比倾向于2∶2；如果是三聚体，则提示ASICs的装配是随意的，分子比是不固定的，2∶1和1∶2这两种情况都存在。

The structure analysis showed that: The subunit structure of Irr-GAPDH is very similar to that found in Americanus Homarus lobster GAPDH. It consists of two domains, one is 〓 binding domain which contains typical Rossmann folds.

为0.024〓。结构分析表明：与美国龙虾GAPDH结构相似，四聚体分子中每一个亚基可分为二个结构域。

Further X-ray structure analysis at low resolution using molecular replacement technique with the Homarus Americanus lobster GAPDH structure as model structure shows that the subunit arragement within molecule is 222 symmentry, the molecular Q axis is parallel to crystallographic 2-fold axis b and molecular P and R axes are parallel to crystallographic a and c axes respe-ctively.

以Homarus Amercanus龙虾GAPDH结构为模型结构，应用分子置换技术进行了低分辨率Χ射线结构分析，结果表明：分子内亚基排列具有222对称性，分子Q轴平行于晶体学二重轴b，分子P和R轴分别平行于晶体学a和c轴。

The G α subunit will eventually hydrolize the attached GTP to GDP by its inherent enzymatic activity, allowing it to reassociate with G βγ and starting a new cycle.

G蛋白的作用是分子开关，α亚基结合GDP失活，结合GTP活化。α亚基具有GTP酶活性，可催化结合的 GTP水解，恢复到无活性状态。

Sequences analysis indicated 26 unique cDNA sequences that encoded putative proteins showing similarities to HL35 antigen U, hlim3, heat shock protein, NADH dehydrogenase subunit 1 and calreticulin of H. longicornis, disulfide isomerase of Ambiyomma variegatum and Ixodes scapularis.

结果表明：在长角血蜱雌蜱唾液腺cDNA表达文库中获得26个长角血蜱免疫相关cDNA序列，其编码蛋白与长角血蜱HL35、黏附素hlim3假定蛋白、热休克蛋白、NADH脱氢酶、钙网蛋白以及杂色花蜱和肩突硬蜱的二硫异构酶等具有同源性。

In this work, we isolated the three kind cDNAs of the vacuolar membrane proton-translocating inorganic pyrophosphatase and the B subunit of the vacuolar-type H~+-ATPase and betaine aldehyde dehydrogenase from Kalidium foliatum and Halostachys caspica by RT-PCR and race.

结果为Na~+和脯氨酸都是有效的渗透调节剂，处于低水势的植物细胞仍旧能从细胞外高浓度的盐溶液中吸收水分。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。