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In this study, we have also designed three primers to amplify Jiaozhou Bay dinoflagellate ribosomal RNA small subunit gene fragment.

另外，我们还设计了甲藻核糖体小亚基基因(ribosomal RNA small subunit gene，ssu rDNA)特异引物，对胶州湾表层海水甲藻遗传多样性进行了初步研究。

Immunohistochemical localization of inhibin α-subunit in normal mature rats was carried out by using Strept Avidin-Biotin-Peroxidase Complex .The results showed that there was positive immunoreactivity of the McAb to inhibinα-subunit in heart ,kidney , spleen , lymph ,adrenal gland and pancreas cells. It indicated that inhibinα-subunit existed in the above tissues , But no positive sites were seen in hypothalamus, pituitary, lung and liver, that means they have no inhibinα-subunit. Compared with the ovariectomized rats, the hypothalamus and pituitary of the rats injected with inhibin-α fragment（1～32）showed an positive reaction, suggesting that inhibin α-subunit could pass through the blood-brain barrier .

用链霉亲和素-生物素-过氧化物酶(Strept Avidin-Biotin-Peroxidase Complex， SABC)免疫组织化学方法，以抑制素α亚基单克隆抗体对正常雌性性成熟大鼠各组织的抑制素α亚基进行定位观察，结果显示：⑴大鼠心脏、肾、脾、淋巴、肾上腺、胰腺均呈阳性反应，表明存在抑制素α亚基；下丘脑、垂体、肝脏和肺组织呈阴性反应，表明其不存在抑制素α亚基；⑵与去卵巢大鼠相比，外源注射抑制素α（1～32）片段的大鼠其下丘脑和垂体出现阳性反应，说明抑制素α亚基能通过血脑屏障。

The troponin I subunit was used as a molecular marker to explore the relationship between the resting intracellular Ca2+ concentration and myofibril degradation in muscle fibers.

本文以肌钙蛋白抑制亚基(troponin I subunit， TnI)作为肌节蛋白降解的分子标记，探讨舒张期肌纤维内Ca2+浓度升高与肌原纤维降解的关系。

At least three clear bands were obtained by sodium dodecyl sulfate polyacry- lamide gel electrophoresis, a subunit and 13 subunit of phycoerythrin, y subunit of phycoerythrin and one subunit of R-phycocyanin.

SDS-PAGE电泳鉴定至少有三条明显的带，为PE的α和β的重叠带，γ亚基和RPC的亚基。

Cloning and sequencing of the genes coding a and 3 subunit of phycoerythrin of a red alga-Corallina officinalis are reported firstly(GenBankaccession number: AF510986). The sequence contain 1,140 nucleotide, including 10 nucleotide that locate in the upstream of the initiation codon ofP subunit, 534 coding for P subunit(coding 177 amino acid), 101 nucleotide for gene spacer and 495 nucleotide that draw near the 5 terminal of a subunit(coding 164 amino acid).

首次测定了珊瑚藻藻红蛋白α和β亚基基因的全序列(GenBank登录号：AF510986)，测定的基因序列长为1，140个碱基，包括β亚基起始密码子上游的10个碱基，6亚基编码区的534个碱基(编码177个氨基酸)，101个碱基的间隔区以及α亚基编码区近5'端的495个碱基(编码164个氨基酸)。

RT-PCR and Real-time PCR analysis revealed that mRNA levels of the FK506-binding protein 12 (FKBP12) and two unknown genes were higher in the diapausing pupae, whereas the ecdysteroid-regulated 16kDa protein (esr16), NADH dehydrogenase 1 alpha subcomplex subunit 6, and two unknown genes were higher in the pupae of diapause termination.

通过RT-PCR和Real-time PCR的方法，鉴定了滞育解除过程中有3个基因表达量在下调，有4个基因表达量在上调。这7个差异表达基因中有3个和已知的基因有较高的同源性：FKBP12，esr16和NADH dehydrogenase 1 alpha subcomplex subunit 6。

Therefore, in the present study, a retrograde and anterograde experiments were made in the rat to address this issue. After injection of cholera toxin B subunit, a retrograde tracer, into Barrington's nucleus or D-region, some retrogradely labeled neurons were found in lamina I, sacral parasympathetic nucleus and dorsal commissural nucleus of the lumbosacral spinal cord segments. Injection of biotinylated dextran amine into lamina I, sacral parasympathetic nucleus and dorsal commissural nucleus, respectively, a large number of BDA-labeled axon terminals were seen in Barrington's nucleus and D-region.

发现，将逆行示踪剂霍乱毒素 B 亚单位（ Cholera toxin B subunit； CTb ）注射到 Barrington's 核或 D 区后，在大鼠腰骶髓 I 层、骶髓副交感核和后连合核内分布有一定数量的 CTb 逆标神经元；将顺行示踪剂结合生物素的葡聚糖胺(Biotinylated dextran amine； BDA)分别注射到腰骶髓 I 层、骶髓副交感核和后连合核内后， Barrington's 核和 D 区内出现大量顺行标记的轴突终末。

Therefore it is interesting to clone genes related to lemma and palea. In this thesis, six genes from the lemma/palea related gene pool which is established by Liu (2003) were further studied. Those genes are salT gene (salt-induced protein), GA-SPY gene (gibberellin action negative regulator SPY-related protein), U2AF gene (U2 snRNP auxiliary factor, small subunit-related protein), kinesin-like gene, DnaJ-like gene, and EF hand gene. At first, the gene expression in leaves and 1~4 cm inflorescences of normal lemma/palea, smaller lemma/palea, and stunted lemma/palea was compared by Real Time RT-PCR.

从刘(2003)所建立水稻颖花发育相关候选基因库中，挑选6个基因：salT基因(salt-induced protein)、GA-SPY基因(gibberellin action negative regulator SPY-related protein)、U2AF基因(U2 snRNP auxiliary factor，small subunit-related protein)、kinesin-like基因、DnaJ-like基因、及EF hand基因，以水稻正常型颖花、小颖花突变体及内外颖退化突变体之叶片及1~4公分幼花序为材料，利用Real-Time RT-PCR分析，发现salT基因在突变体幼花序中大量表现。

The prestent invention discloses the cDNA sequnce of a new human reduced NADH: ubiquinone oxidoreductase 14 KDa subunit (NADH-CoQ14KDa subunit). The protein coded with the sequence is the homolog of ox's NADH-CoQB14.5b subunit.

本发明提供了一种新的人还原型烟酰胺腺嘌呤二核苷酸－辅酶Q氧化还原酶14千道尔顿亚基（NADH：ubiquinone oxidoreductase 14 kDa subunit，简称为&NADH－CoQ 14 kDa亚基&的cDNA序列，该序列编码的蛋白是牛NADH－CoQB14.5b亚基的同系物。

These results suggested that in chloroplast ATP synthase, both the N-terminus and C-terminus of the ε subunit show importance in regulation of the ATPase activity. Furthermore, the N-terminus of the ε subunit is more important for its interaction with γ and some CF〓 subunits, and crucial for the blocking of proton leakage. Compared with the ε subunit from E. coli, the chloroplast ε subunit is more sensible to N-terminal or Cterminal truncations.

从这些实验结果中可以看出：（1）叶绿体ATP合酶ε-亚基的N端和C端对于其功能的发挥都很重要；（2）ε亚基N端和C端氨基酸在抑制ATP水解和堵塞质子泄漏两个功能上起着不同的作用；（3）与细菌ATP合酶相比，叶绿体ATP合酶ε亚基N端和C端的缺失对其功能的影响更为显著。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。