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Results Same conservative sites and key catalytic sites existed among SjLDH and LDHs from other species. Similarity of SjLDH compared to CsLDH, TvLDH and HsLDH was 75%, 17%, 58%-60% respectively. Phylogenetic analysis demonstrated that the evolution relation between SjLDH and DmLDH was closer than the relation between SjLDH and CeLDH, the relationship between SjLDH and HsLDH-B,-C was closer than HsLDH-A. Three trans-membrane regions were found, the region of 98aa-106aa in three hydrophilia regions located outside of membrane was inferred as the major antigen epitope. This antigen epitope had significant difference with LDHs from protozoon （ Pf ., Tg ., Tv .） and had 1-3 amino acid residue difference with MmLDH, HsLDH-A,-B,-C, and was the same with CsLDH. One of the key catalytic residues and substrate binding loop were located in this region. Tertiary structure demonstrated that 98aa-106aa was on the surface of the protein and formed a substrate binding loop, other two key catalytic sites were at the position near the loop.

结果 SjLDH与其他物种的同源序列含相同的保守位点及催化活性位点，与华支睾吸虫LDH同源性最高为75％，与阴道毛滴虫LDH同源性最低为17%，与人LDH（HsLDH-A，-B，-C）的同源性为58%~60％； SjLDH与果蝇的进化距离较秀丽隐杆线虫为近，3种人LDH中与HsLDH-B、-C的进化距离较HsLDH-A为近；该蛋白具有3个跨膜区域，3个高亲水性区域，主要的线性表位98aa~106aa位于膜外，与原虫LDH相同区域差异显著，而与其他LDH有1~3个氨基酸的差异，关键催化位点之一及底物丙酮酸结合区域位于该区域，蛋白质同源模建分析表明该区域位于蛋白表面形成环状结构，3个关键催化位点位于该区域或在其附近。

The optimum hydrolytic conditions of razor clam for neutral protease were : enzyme concentration 2.4%, hydrolysis temperature 50癈, substrate concentration 1:3, pH 7 and reaction time 6 hrs with the degree of hydrolysis and extraction rate of total nitrogen 42.46% and 83.04% respectively; and foracid protease they were: en/yme concentration 6.0%, hydrolysis temperature 50癈, substrate concentration 1:4, pH3.5 and reaction time 5 hrs with the degree of hydrolysis and extraction rate of total nitrogen 46.37% and 87.94% respectively, the hydrolysate being clear light yellow. For the combination of two kinds of proteases they were: first hydrolyzing with 2.6% f 1 avouryme for 3 hrs, then hydrolyzing with 2.4% neutral protease for 3 hrs with the degree of hydrolysis and extraction rtiie of total nitrogen 4-1.57% and 86.95% respectively.

最后综合考虑了水解率、总氮回收率和水解液的色泽、澄清度等指标，得出了较适宜的酶种为单酶以枯草杆菌中性蛋白酶和酸性蛋白酶效果较好，双酶以风味蛋白酶与枯草杆菌中性蛋白酶双酶组合水解效果较好，其适宜的水解工艺条件分别为：枯草杆菌中性蛋白酶加酶量为2.4%，水解温度50℃，料水比1：3，pH值中性条件下水解6h，绕蛙蛋白质的水解率和总氮回收率分别为42.46%和83.04%；酸性蛋白酶水解编蛙肉的适宜的工艺条件为加酶量6.0%、水解温度50℃，料水比l：4，pH值3.5条件下水解sh，水解率为46.37%，总氮回收率为87.94%，水解液呈较清澈的淡黄色；双酶采用先用2.6%风味酶水解3h后，再加入2.4%中性蛋白酶水解3h，水解率达44.57%，总氮回收率为86.95%。

The relative permittivity of the Langmuir monolayer on a substrate surface is calculated by the orientation order S with consideration of the length of the permanent dipoles within domain and the distance between the dipole and the substrate.

计算结果表明：对于成份一定的稳定构型单分子层膜，其相对电容率大小除了依赖于分子密度和基底物质的相对电容率外，对分子负电荷中心离基底的距离，以及考虑电偶极子长度的分子区域大小也有不同程度的依赖

Epitaxial growth of GaN on sapphire and novel substrate LiAlO〓 1 The growth mechanism of GaN on sapphire substrate was investigated using AFM, X-ray diffraction, X-ray double crystal diffraction, Rutherford back scattering etc.

阐明了衬底表面的氮化处理、缓冲层的生长对外延膜结晶形貌、结晶质量、以及外延匹配关系的影响规律。

The results showed that all components had low substrate affinity for CMC, and V〓 can be reached only at higher substrate concentration. The K〓 value of βglucosidase to salicin is 0.2%.

通过对各酶组分动力学参数的测定，五种内切酶对底物CMC-Na的Km值分别为（从EGⅡ-1到EGⅣ）；6.8％、4.6％、6.8％、8.7％和5.9％，说明这五种内切酶对底物的亲和力较小，需要在较高的底物浓度下才能达到Vmax；β-葡萄糖苷酶对底物水杨素的Km值为0.2％。

In this research, the activities of CWDE were measured by saccharifying according to the theory that red-brown amino compounds were produced when 3, 5-dinitro salicylic acid and aldehyde were heated together. And the testing conditions were made clear. The activity of Cx should be tested with optimal temperature of 50℃, optimal pH of 4.8, incubation time of 30 min, and optimal substrate of 0.6% CMC-Na. Due to the heat-libality, Cx should be sterilized more than 10 min. The testing conditions of the activity ofβ-G were almost the same with Cx except the substrate of 0.5% salicin and the sterilization time of more than 15 min.

主要取得了一下成果：(1)明确了条锈菌细胞壁降解酶活性测定的条件：羧甲基纤维素酶活性的测定条件为50℃，pH4.8，反应时间30min，底物浓度为0.6%羧甲基纤维素钠，以DNS法测定，且失活酶液的灭活时间应大于10min；β-葡萄糖苷酶活性的测定条件为：50℃，pH4.8，反应时间30min，底物浓度为0.5%水杨苷，以DNS法测定，且失活酶液的灭活时间应大于15min；果胶酶活性的测定条件为：50℃，pH4.8，反应时间1h，底物浓度为0.25%果胶，以DNS法测定，且失活酶液的灭活时间应大于10min。

Study on the optimal conditions of cellulase from termites The optimal pH when Cx from Reticulitermes flaviceps reacted with substrate sodium carboxymethylcellulose was between 5.0-7.4 and 5.0-6.2 when BG enzyme was reacted with substrate salicin, the relation between activities of enzymes and relevant pH values accorded with Quadratic models.

一、白蚁纤维素酶最适反应条件研究黄胸散白蚁 Reticulitermes flaviceps C_N酶与羧甲基纤维素钠(Carboxymethylcellulose sodium，CMC-Na)反应的最适pH值范围在5.0-7.4之间，BG酶与Salicin反应的最适pH值为5.0-6.2，2种酶的活性均与pH值符合二次项曲线模型。

The results indicated that substrate sandblast contributed to enhance the adhesion between the titanium substrate and the MMO coating, while increasing in the coating mass led to increased durability of the anode, and the coating consumed rate of the coated anode was almost zero.

所研制的RuIrTiSnCo涂层钛阳极具有良好的电化学稳定性和较长的寿命，强化寿命达到96h，约为次氯酸钠发生器标准的5倍，是海水介质外加电流阴极保护中比较理想的辅助阳极。

Design of beam delivery system for RF CO_2 laser ceramic substrate scriber;2. The characteristic of RF CO 2 laser and its ceramic substrate scribing are pre sented.

介绍了RFCO2激光器及其陶瓷基板划片的特点；分析了RFCO2激光陶瓷基板划片的机理，给出了整机总体设计方案。

The indexes of refraction of the material of the fibers in the scrim and of the adhesive are similar so that when the layer of adhesive is pressed against a substrate through the backing layer, the adhesive will more totally wet the fibers of the reinforcing scrim, causing them to become significantly less visible in the reinforced tape adhered to the substrate.

稀松无纺织物纤维材料和粘合剂的折射率是相似的，当粘合剂层通过背衬层被按压在基片上时，粘合剂会更完全地润湿增强的稀松无纺织物纤维，使其在粘贴于基片上的增强胶带中变得明显更不可见。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。