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The eluate was prepared by dissolving am- monium formate 6.3 g and octanen sulfoacid sodium 1.0 g in 700 mL water(pH 3.8 adjusted with formic acid)and subsequently diluted with water to 1000 mL.

结果：在本色谱条件下腺苷蛋氨酸与辅料、溶剂峰及其有关物质的分离度符合要求，在200～600μg·mL~(-1)范围内线性良好(r=0.9998)。回收率为99.61%～99.98%，RSD为0.23%～1.3%。

Subsequently, We studied the Duursma-Lee algorithm and the modified Duursma-Lee algorithm that are algorithms of computing Tate pairing of supersingular elliptic curve over finite fields with characteristic three.

其次，本文对计算特征为3的有限域上超奇异椭圆曲线Tate Pairing的Duursma-Lee算法和Modifled Duursma-Lee算法进行了研究。

The tea extract which has been treated with tannase is subsequently subjected to an ultra-filtration treatment.

随后，用丹宁酶处理过的茶叶提取物要经过超过滤处理。

Subsequently, the police confirmed that the Taxodiaceae Ningbo, China Light and sung in Tianjin, such as grain and oil companies have de facto control of the same people - businessmen in Zhejiang Province Wang Wei.

随后，警方证实，宁波杉科，中国轻，在天津，宋如粮油公司的实际控制人同在浙江省汪炜-商人。

Subsequently, a l kb fragment of HCCR-2 encoding region was amplified by RT-PCR and cloned into Promega TEasy vector.

测序证实无误后，以HCCR-2／T载体为模板，再通过PCR克隆出HCCR-2的编码区序列，将其连接到pIRES2-EGFP真核表达载体，并通过测序获得证实。

Methods: total rna was isolated from hepatocellular carcinoma cell line hepg2. subsequently, a 1kb fragment of hccr 2 encoding region was amplified by rt pcr and cloned into promega teasy vector. after confirmed by dna sequencing, the full length encoding region of hccr 2 was amplified by pcr and cloned into pires2 egfp. the recombinant eukaryotic expression vector was confimed by dna sequencing.

从人肝癌细胞株hepg2提取rna，利用rt pcr方法，先克隆出一包括hccr 2编码区的长约1 kb的片段，并将其与t载体连接并转化，测序证实无误后，以hccr 2/t载体为模板，再通过pcr克隆出hccr 2的编码区序列，将其连接到pires2 egfp真核表达载体，并通过测序获得证实。

METHODS: Total RNA was isolated from hepatocellular carcinoma cell line HepG2. Subsequently, a 1kb fragment of HCCR2 encoding region was amplified by RTPCR and cloned into Promega TEasy vector. After confirmed by DNA sequencing, the fulllength encoding region of HCCR2 was amplified by PCR and cloned into pIRES2EGFP. The recombinant eukaryotic expression vector was confimed by DNA sequencing.

从人肝癌细胞株HepG2提取RNA，利用RTPCR方法，先克隆出一包括HCCR2编码区的长约1 kb的片段，并将其与T载体连接并转化，测序证实无误后，以HCCR2/T载体为模板，再通过PCR克隆出HCCR2的编码区序列，将其连接到pIRES2EGFP真核表达载体，并通过测序获得证实。

Neptune was subsequently found within a degree of its predicted position, and its largest moon, Triton, was discovered shortly thereafter, though none of the planet's remaining 12 moons was located telescopically until the 20th century.

海王星后来在其预测的位置度被发现了，其最大的卫星海卫，不久也被发现，而其余12个卫星直到20世纪才被确切的定位。

A wind- and motor-powered land vehicle with a fore-and-aft longitudinal axis comprising a body supported by wheels, at least one of said wheels fixed to rotate in a plane parallel to the fore-and-aft axis of said vehicle, an airfoil system comprised of two vertically extending airfoils rigidly mounted on said body in an arrangement having bilateral symmetry about a vertical plane through said fore-and-aft axis, said airfoil system providing a forward propelling force of said vehicle when the airfoil system is subjected to certain cross wind conditions, and self-contained motor propulsion means on said vehicle for providing acceleration from a stationary position and for propelling the vehicle either independently or in combination with propelling forces generated by wind action on said airfoil system, each airfoil having a plurality of airfoil sections telescopically fitted together, the lower airfoil section being rigidly mounted in fixed relationship to said body, said airfoil system including a retracting mechanism for lowering upper airfoil sections of said airfoil system when desired and for subsequently extending said airfoil system, said retracting mechanism including a plurality of linkages interconnecting said sections and an actuator means operatively connected between said body and to said linkages for lowering and extending each airfoil, and an intermediate airfoil extending between the upper sections of each of said airfoils so as to provide an inverted U-shaped airfoil.

风力和电动机驱动的陆地车辆与前列和尾部长轴组成的一个机构的支持，车轮，至少有一说，车轮固定在一个旋转平面平行的前列和前后轴的说，车辆，翼型系统组成的两个垂直扩展翼型硬性安装在说，机构在安排后，双边对称约一垂直面说，通过国内外和前后轴，表示翼型系统提供了一个向前推进的力量说，车辆时，翼型系统是受到某些横风条件下，和自我载电机推进的手段就表示，车辆提供加速从一个固定的立场和为推进车辆单独或结合，与推进力量所产生的风的行动表示翼型系统，每个机翼有一个多元化翼型第telescopically装在一起，较低的翼型节被硬性安装在固定的关系，说身体，说翼型系统包括一个撤消的机制，降低上翼型章节说，翼型系统时，理想的和其后再将说，翼型制度，说收回机制包括多元化的联系互连说，部分和一个致动器的手段手术之间的连接说，身体和说，联系降低，并延长每翼型，及一个中间翼型延长之间的路段上的每说翼型，以便提供一个倒U -形翼型。

Subsequently, the feasibility of the processes of the catalytic reduced dechlorination were affirmed in a pilot scale by the treatment of the industrial wastewater containing chloroform and tetrachloromethane from the fluorine industrial factory of Zhejiang Juhua Corporation.

在此基础上，以浙江衢化氟化学有限公司排放的有机氯工业废水为处理对象，研究了实际工业废水中氯仿和四氯化碳的脱氯效果和废水处理的可行性。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷