查询词典 submitted

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

Following this decision, a neighborhood newsletter article was submitted by a property owner opposing the removal of native Sonoran desert plant species.

依照这个决定，一位房主投稿给街区新闻通讯登了一篇文章，反对除掉本地的北美沙漠植物物种。

Those committees researched the comments submitted by the public and passed their summaries to Stallman for a decision on what the license would do.

这些委员会研究提出的评论，公众，并通过他们的摘要，以Stallman的一个决定，对什麼许可做。

The nearly 160 poems submitted in the Pittsburgh contest include hopeful messages, congenial welcomes, Pittsburgh Steeler cheers and a mix of political messages and pleas for peace.

一些作品似乎完全忽视了G20这个主题，比如一位曾在Allegheny County监狱服刑的犯人创作的

The software submitted for 1-dimentional simulation can simulate the controlling on the stratigraphic pattern of different factors.

建立的一维地层形成样式模拟可分析不同因素对地层叠加样式的影响，为二维和三维地层模拟奠定了基础。

Due to frequent misunderstandings, STUT submitted a successful proposal to the Executive Yuan 3 years ago asking that TUT change its name.

由於误会实在太频繁，南台科大3年前向行政院提出诉愿成功，要求台南科大改名。

Available space for symposia is limited and submitted requests will be reviewed on a firstcome, first-served basis.

可用空间专题讨论会是有限的，并提交要求将检讨有关firstcome ，先到先得。

While sneering at the childish attempts of his talentless mail-order "pupils", he falls in love with the artistic beauty of a religious painting submitted to him by his sole pupil of promise: an ageless, faceless nun.

虽然幼稚嘲讽他的企图talentless邮购&学生&，他爱上了艺术美的宗教绘画提交给他，他唯一的学生的承诺：一个永恒，不露面的尼姑。

Leverkusen, Germany-based regional co Temin's German subsidiary have 4 to bankruptcy, the company has four subsidiaries located in the Administrative Court submitted an application to enter bankruptcy procedures.

总部位于德国Leverkusen地区的泰明顿公司旗下有4家德国子公司将破产，公司已向四家子公司所在的行政法院提交了进入破产程序的申请。

However, a power of attorney signed by the legal representative of each consortium member shall be submitted to the tenderee.

但是，需要向招标人提交由所有联合体成员法定代表人签署的授权委托书。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。