查询词典 subline

Fold resistance to the antimetabolites. the subline, however, was not resistant to alkylating agents,platinum compounds, and other naturally-derived antineoplastics.

该细胞株对抗代谢药产生6.70倍的耐药，但对多种其他天然来源的抗肿瘤药物、烷化剂以及铂类化合物则缺乏交叉耐药性。

The same procedure was repeated 5 times until a new cell subline, designated as SW480/M5, was established, whose biological behaviors was investigated both in vivo and in vitro.

采用外科原位接种、组织块细胞培养法从裸鼠体内获得SW480细胞系中具有肝脏转移倾向的亚克隆SW480/M5；体内体外实验验证它与母系细胞在生物学特性上的差异。

Methods Using recombinant adeno viruses,we expressed the B7.1 gene in murine breast tumor cell line EMF6 and a subline previously transfected with retrovirus vector XdF containing IL-2-TNFα fusion gene.

在小鼠乳腺癌细胞系EMF6和一个已被含有IL-2和TNFα融合基因的逆转录病毒载体XdF转染的EMF6亚系(T2-EMF6)中表达B7.1基因。

MethodsA subline of human pancreatic cancer cell line MIAPaCa2 (MIAPaCa23) with high liver metastasis potential was selected and injected through the tail vein to establish a pancreatic cancer model in nude mice with high liver metastasis. The capability of invasion in vitro was evaluated by a transwell chamber system.

方法筛选出高肝转移的胰腺癌细胞亚型MIAPaCa23，用Transwell小室模型观察PESV对MIAPaCa23细胞体外侵袭能力的影响；尾静脉注射MIAPaCa23细胞建立高肝转移率的裸鼠胰腺癌模型，对比PESV和化疗药物吉西他滨以及抗血管生成剂TNP470对裸鼠胰腺癌肝转移的抑制作用。

METHODS: CNE-2 human nasopharyngeal carcinoma cells were exposed repeatedly to γ-rays and then screened for a new radioresistant subline named CNE-2R. Double times of CNE-2 and CNE-2R cells were determined by MTT assays. The relative radiosensitivities of tumor cells were assessed by standard colony formation assays. Survival rates after exposed to irradiation were measured by MTT assays. The time courses of cell cycle distribution before and after irradiation were investigated by flow cytometry.

用γ射线反复照射CNE-2细胞，筛选出放射抗拒细胞株CNE-2R；检测细胞生长的倍增时间；应用细胞克隆形成实验检测细胞的放射敏感性；MTT法检测照射后不同时间的存活分数；用流式细胞术检测细胞的周期分布特征。

A marked accumulation of cells in G2/M phase was observed in CNE-2R cells during the period from 12 to 36 h after irradiation A little increase of cells in G2/M phase was observed in CNE-2 cells from 12 to 24 h after irradiation. CONCLUSION: The new subline CNE-2R is more radioresistant comparable to its parental CNE-2R cell line, and has a different cell cycle distribution.

人鼻咽癌细胞株CNE-2经间歇性大剂量γ射线多次照射后得到的CNE-2R具有稳定放射抗拒性，并且显示出与亲代不同的细胞周期特征。

See by Chloe is Chloe's Women's subline series. See by Chloe caters for young girls taste, which is a little Rock'n'Roll rebellious, and the grace of Chloe.

See by Chloe 是 Chloe 旗下的女装副线系列， See by Chloe 的女性是年轻潮流的女孩，有一点 Rock'n'Roll 的调皮叛逆，但又不失珂洛艾伊的高级品味。

The dance of the Tang Dgnasty developed as prosperously as the social dolitoped as prosperosusly as the social politices if possessed the qualify of Subline and magnificence, which is the example of the classical qualify, they regard the harmony as the highest beauty.

唐朝时期舞蹈与唐朝兴盛发达的社会政治一样具有泱泱大国之风、中正、博大、庄严、高雅，在艺术中求人生之至善，以隽淡的情怀，为创作的心性，以内省的哲理为舞蹈的意境，以敬化天下。

Methods］ MTT assay was employed to test the lethal concentration 50 (IC50) of sensitive germline (SK-OV-3) and ovarian cancer cell subline induced by ADR (SK-OV-3/adr) treated by 4 drugs, resistance index and reverse multiple were calculated. The changes of drug content were observed by fluorescence microscope, the intracellular accumulation of ADR was evaluated by fluorospectrophotometry, the changes of mdr 1/P-gp resistance were observed respectively by RT-PCR and Western blot.

方法］MTT法检测药物对敏感细胞株（SK-OV-3）和诱导的耐药细胞株（SK-OV-3/adr）的半数致死浓度（IC50），计算耐药指数和加逆转剂后的逆转倍数，荧光显微镜观察不同时间细胞内的药物含量变化，荧光分光光度法测定细胞内ADR的含量，RT-PCR和Western blot法检测细胞耐药前后mdr1/P-gp的变化。

Methods Hela cells of human cervical cancer were cultured with mitomycin C in gradually increased dosage in order to obtain a drug-resistant Hela/MMC cell subline. The biological properties of Hela and Hela/MMC cells were compared and the mechanism of drug-resistance of Hela/MMC cells studied.

以人宫颈癌Hela细胞为亲本，采用丝裂霉素连续性药物作用，间隙性增加药量，逐步提高药物浓度的方法，分离出MMC耐药亚系，并比较两种细胞生物学特性差异。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新