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subcultures相关的网络例句

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Plantlet regeneration from cotyledon of Citrullus lanatus cv. Zhangkang No. 4 was studied. The results showed that aseptic seedlings should be cultured in dark for 3d, then exposed to a photoperiod of 16/8h/d for 3d. The callus induction rate was higher in cotyledons placed facing downwards to the medium than in those facing upwards. The highest induction rate occurred in MS medium containing 6-BA (2.0mg/L), kinetin (1.0mg/L)and GA3 (1.0mg/L). The induction initiated in dark and took 7 days, while callus growth and differentiation proceeding for 7 days under 16/8h light-dark cycles. A highest rate of embryogenic callus was obtained after 3 successive subcultures.

以无子西瓜郑抗4号无菌苗子叶为外植体,进行了体细胞胚发生及植株再生的研究,结果表明,无菌苗应先进行3d暗培养,然后采取光照16h/d和黑暗8h/d培养3d;将子叶的叶面朝下放置于培养基上的愈伤组织诱导率高于叶面朝上的培养方式;子叶诱导胚性愈伤组织的最适培养基配方为MS+6-BA2.0mg/L+KT1.0mg/L+GA31.0mg/L;诱导需要在黑暗条件下启动,进行7d暗培养,而生长分化于光照16h/d和黑暗8h/d条件下培养7d;继代3次得到的胚性愈伤组织最多;最适生根培养基配方为1/2MS+IBA0.3mg/L。

Results The latent phase of the primary culture cells was 5-9 days, followed by 11-16 days of logarithmical proliferation; the plateau began at day 18-20. The subcultures showed 12-24 hours of latent phase, followed by 7-10 days of logarithmical proliferation, then entered the plateau (11-13 days). The hMSC expressed positive CD44 unanimously, but don′t expressed CD45. Low concentration of bFGF (10 μg·L-1) could stimulate the proliferation of hMSC significantly (P.05); while 25-100 μg·L-1 of bFGF could inhibit the ALP activity.

结果:原代培养细胞生长潜伏期5~9 d,对数增殖期11~16 d,生长平台期18~20 d;传代培养细胞生长潜伏期12~24 h,对数增殖期为7~10 d,11~13 d进入细胞生长平台期。hMSC均一表达CD44,不表达CD45.bFGF在较低浓度(10 μg·L-1)时即有显著的促进hMSC增殖的作用(P.05), bFGF在25~100 μg·L-1对hMSC的ALP活性有较显著的抑制作用。

All of the hygromycin B-resistant transformants tested were mitotically stable after several subcultures onto complete medium without hygromycin.

用大麦叶片离体接种的方法快速测定部分稻瘟病菌转化子的致病性,发现一个致病缺陷突变体:Al-412。

Grisea. All of the hygromycin B-resistant transformants tested were mitotically stable after several subcultures onto complete medium without hygromycin.

该突变体不能侵入水稻叶片及擦伤的大麦或水稻叶片,说明Al-412突变体在寄主组织中扩展进程被阻断。

The friable embryogenic callus with small grains and without proembryo formation was screened in the process of continuous subcultures for 4~6 times on the medium with low content of sucrose from embryogenic cultures induced from immature embryos in litchi (Litchi chinensis Soon.). The well-scattered embryogenic cell suspensions could be rapidly established from the friable embryogenic callus in 2 liquid initial MS media respectively supplemented with 2,4-D 2 mg/L or 2,4-D 2 mg/L, KT1 mg/L and AgNO3 5 mg/L shaking at 100~120 r/min within 10-14 days.

荔枝幼胚诱导的胚性培养物在低糖条件下连续继代4~6次左右,可筛选到颗粒细小、不含原胚的松散型胚性愈伤组织;以这种松散的胚性愈伤组织作为起始材料,在附加2,4-D 2mg/L或2,4-D 2mg/L、KT1 mg/L、AgNO3 5mg/L的MS液体启动培养基上振荡培养(100~120 r/min)10~14 d,即可建立起分散性良好的胚性悬浮细胞系。

In the "golden '70s" both were part of cradle-to-grave subcultures whose members worked, holidayed and voted together.

在&黄金70年代&,两者都是&从摇篮到坟墓&的一部分一群人,他们一起工作,一起度假,一起投票。

In the "golden '70s" both were part of cradle-to-grave subcultures whose members worked, holidayed and voted together.

在&黄金70年代&时,两党的党员都身处于生命周期理论亚文化群中,他们一起度假,一起参加选举。

The cells were passaged into subcultures. They grew to confluence 6 days later again.

细胞大多呈六角形,较稀疏的地方细胞呈长梭形。

She then exposes the influence of lesbian drag king cultures upon hetero-male comic films, such as Austin Powers and The Full Monty, and, finally, points to dyke subcultures as one site for the development of queer counterpublics and queer temporalities.

然后,她公开呼吁异男漫画电影,如奥斯汀的权力,全部蒙蒂,以及最后的女同志拖到国王文化的影响,作为一个点,堤防为酷儿counterpublics奇怪时间性和亚文化发展的网站。

By putting forward his Trading Zone theory, Peter Galison argues that the disunity of scientific structure, which is demonstrated by it's subcultures and the local coordination among them occuring within the area of the trading zone, undergirds the strength and stability of science.

关于科学的基本性质和内部结构问题,盖里森在分析和批判逻辑实证主义和反实证主义传统观点的基础上,通过借鉴人类学的研究方法,提出了一种互嵌式的科学结构模式和存在于科学亚文化层面上的&交易区&理论,进而阐述了科学的分立性。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?