查询词典 subculture

The fungi was isolated and purified from polluted anthurium subculture medium, identified to be Deuteromycotina Hyphpmycetes Hyphomycetales Dematiaceae and trichoderma. The inhibition effect of the three kinds of chitosan on the fungi was conducted.

在污染的红掌继代培养基中提取污染菌并进行了分离及纯化，初步鉴定为为半知菌亚门，丝孢纲，丝孢目，淡色孢科，木霉属。

Many embryogenic calli were produced through subculture by calli induced from mature embryo of rice.

从水稻成熟胚诱导出的愈伤组织经继代培养后可以产生大量的胚性愈伤组织。

Mscs were cultured in a conditional medium in subculture including: dexamethasone, vitamin c, and β-glycerophosphate.

sd大鼠12只，在全麻及无菌条件下取其左侧股骨的骨髓基质细胞，并用矿化液诱导培养。

MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs，G-418筛选阳性细胞克隆，将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上，体外培养7天后，植入原大鼠肌肉内。

Objective: To optimize the culture system of hEGcs in vitro, select the hEGcs from subculture to transplant directly to the rat infarcted myocardium and observe the therapeutic mechanism of their heterotransplantation in the treatment for the rat myocardial infarction.

目的：优化人胚胎生殖细胞的体外培养体系，并将生长状态良好的传代的人EG细胞移植用于大鼠心肌梗死模型的治疗，探讨人胚胎生殖细胞进行异种移植对大鼠心肌梗死的治疗机制。

It was shown that the resultant cybrid HL-R cells incorporated with reticulocyte cytoplasts became differentiated 〓 bytalterationcin growth parameters, which might be defined as decancerization:(1) loss of growth ability in soft agar medium;(2) nontumorigenic under heterotransplantation in their filial cellular generation to mude mice;(3) decrease of growth rate, cellular mitoxic index and DNA synthesis rate;(4) decrease or inhibition of c-myc gene expression;(5) expression of human globin gene products in 〓 subculture.

利用C-myc癌基因探针的分子杂交技术，未检测到胞质杂交细胞存在相当于C-myc基因特异的同源序列mRNA，提示杂交细胞癌基因表达严重受抑。

Methods The tissues from human ovarian carcinoma biopsies were cultured in RPMI1640 media. After growing to 90% confluence, the cells were indigested for subculture, whose characteristics, including morphology, growth kinetic and tumorigenecity in nude mice, were studied.

取手术切除的卵巢癌组织，进行组织块培养法，待长满90%汇合面后消化传代培养，并进行形态学、生长动力学及致瘤性等生物学特性分析。

Kinds of collections of fermented foods;②subculture technics of collections of fermented foods;③disposal and fermentable preparation of fermented materials;④metabolism and metabolic control of microbic fermentation;⑤technics and detections of kinds of fermented foods;⑥separations and distillations of fermented foods;⑦typic clarifications of kinds of fermented foods fully.

主要内容有：①各类发酵食品的菌种；②发酵食品菌种的扩大培养技术；③发酵原料的处理与制备工艺；④微生物发酵的代谢及调控；⑤发酵类食品的生产工艺及产品的检测；⑥发酵食品的分离提取等；⑦对各类发酵食品的典型实例作全面的阐述

Firstly, callus induction and subculture for Mikania micrantha were studied systematically. The research on callus induction showed that: Shoot tip has higher induction rate than other explants ; At 25℃ and dark regime, the callus was better cultivated on Murashige and Skoog medium (pH5.8) containing 2.0mg/L 2,4-D and 1.0mg/L KT.

首先，对微甘菊愈伤组织诱导和继代增殖培养进行系统研究，愈伤组织诱导研究表明：外植体中芽尖诱导率最高，在含2，4-D2.0mg／L、KT1.0mg／L、pH5.8的MS培养基上25℃黑暗条件下愈伤组织诱导效果较好。

In general, the deviant subculture has played a role in the moralization and collectivity on crimes.

总体而言，越轨亚文化对犯罪有着教化和群体化作用。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。